Large-scale de novo DNA synthesis: technologies and applications

For over 60 years, the synthetic production of new DNA sequences has helped researchers understand and engineer biology. Here we summarize methods and caveats for the de novo synthesis of DNA, with particular emphasis on recent technologies that allow for large-scale and low-cost production. In addi...

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Detalles Bibliográficos
Autores principales: Kosuri, Sriram, Church, George M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7098426/
https://www.ncbi.nlm.nih.gov/pubmed/24781323
http://dx.doi.org/10.1038/nmeth.2918
Descripción
Sumario:For over 60 years, the synthetic production of new DNA sequences has helped researchers understand and engineer biology. Here we summarize methods and caveats for the de novo synthesis of DNA, with particular emphasis on recent technologies that allow for large-scale and low-cost production. In addition, we discuss emerging applications enabled by large-scale de novo DNA constructs, as well as the challenges and opportunities that lie ahead.

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