Large-scale de novo DNA synthesis: technologies and applications

For over 60 years, the synthetic production of new DNA sequences has helped researchers understand and engineer biology. Here we summarize methods and caveats for the de novo synthesis of DNA, with particular emphasis on recent technologies that allow for large-scale and low-cost production. In addi...

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Detalles Bibliográficos
Autores principales: Kosuri, Sriram, Church, George M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7098426/
https://www.ncbi.nlm.nih.gov/pubmed/24781323
http://dx.doi.org/10.1038/nmeth.2918
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author Kosuri, Sriram
Church, George M
author_facet Kosuri, Sriram
Church, George M
author_sort Kosuri, Sriram
collection PubMed
description For over 60 years, the synthetic production of new DNA sequences has helped researchers understand and engineer biology. Here we summarize methods and caveats for the de novo synthesis of DNA, with particular emphasis on recent technologies that allow for large-scale and low-cost production. In addition, we discuss emerging applications enabled by large-scale de novo DNA constructs, as well as the challenges and opportunities that lie ahead.
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spelling pubmed-70984262020-03-26 Large-scale de novo DNA synthesis: technologies and applications Kosuri, Sriram Church, George M Nat Methods Article For over 60 years, the synthetic production of new DNA sequences has helped researchers understand and engineer biology. Here we summarize methods and caveats for the de novo synthesis of DNA, with particular emphasis on recent technologies that allow for large-scale and low-cost production. In addition, we discuss emerging applications enabled by large-scale de novo DNA constructs, as well as the challenges and opportunities that lie ahead. Nature Publishing Group US 2014-04-29 2014 /pmc/articles/PMC7098426/ /pubmed/24781323 http://dx.doi.org/10.1038/nmeth.2918 Text en © Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. 2014 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Kosuri, Sriram
Church, George M
Large-scale de novo DNA synthesis: technologies and applications
title Large-scale de novo DNA synthesis: technologies and applications
title_full Large-scale de novo DNA synthesis: technologies and applications
title_fullStr Large-scale de novo DNA synthesis: technologies and applications
title_full_unstemmed Large-scale de novo DNA synthesis: technologies and applications
title_short Large-scale de novo DNA synthesis: technologies and applications
title_sort large-scale de novo dna synthesis: technologies and applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7098426/
https://www.ncbi.nlm.nih.gov/pubmed/24781323
http://dx.doi.org/10.1038/nmeth.2918
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