Generation of a murine SWATH-MS spectral library to quantify more than 11,000 proteins

Targeted SWATH-MS data analysis is critically dependent on the spectral library. Comprehensive spectral libraries of human or several other organisms have been published, but the extensive spectral library for mouse, a widely used model organism is not available. Here, we present a large murine spec...

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Detalles Bibliográficos
Autores principales: Zhong, Chuan-Qi, Wu, Jianfeng, Qiu, Xingfeng, Chen, Xi, Xie, Changchuan, Han, Jiahuai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Data Descriptor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7099061/
https://www.ncbi.nlm.nih.gov/pubmed/32218446
http://dx.doi.org/10.1038/s41597-020-0449-z
Descripción
Sumario:Targeted SWATH-MS data analysis is critically dependent on the spectral library. Comprehensive spectral libraries of human or several other organisms have been published, but the extensive spectral library for mouse, a widely used model organism is not available. Here, we present a large murine spectral library covering more than 11,000 proteins and 240,000 proteotypic peptides, which included proteins derived from 9 murine tissue samples and one murine L929 cell line. This resource supports the quantification of 67% of all murine proteins annotated by UniProtKB/Swiss-Prot. Furthermore, we applied the spectral library to SWATH-MS data from murine tissue samples. Data are available via SWATHAtlas (PASS01441).

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