Generation of a murine SWATH-MS spectral library to quantify more than 11,000 proteins

Targeted SWATH-MS data analysis is critically dependent on the spectral library. Comprehensive spectral libraries of human or several other organisms have been published, but the extensive spectral library for mouse, a widely used model organism is not available. Here, we present a large murine spec...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhong, Chuan-Qi, Wu, Jianfeng, Qiu, Xingfeng, Chen, Xi, Xie, Changchuan, Han, Jiahuai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Data Descriptor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7099061/
https://www.ncbi.nlm.nih.gov/pubmed/32218446
http://dx.doi.org/10.1038/s41597-020-0449-z
_version_ 1783511285496807424
author Zhong, Chuan-Qi
Wu, Jianfeng
Qiu, Xingfeng
Chen, Xi
Xie, Changchuan
Han, Jiahuai
author_facet Zhong, Chuan-Qi
Wu, Jianfeng
Qiu, Xingfeng
Chen, Xi
Xie, Changchuan
Han, Jiahuai
author_sort Zhong, Chuan-Qi
collection PubMed
description Targeted SWATH-MS data analysis is critically dependent on the spectral library. Comprehensive spectral libraries of human or several other organisms have been published, but the extensive spectral library for mouse, a widely used model organism is not available. Here, we present a large murine spectral library covering more than 11,000 proteins and 240,000 proteotypic peptides, which included proteins derived from 9 murine tissue samples and one murine L929 cell line. This resource supports the quantification of 67% of all murine proteins annotated by UniProtKB/Swiss-Prot. Furthermore, we applied the spectral library to SWATH-MS data from murine tissue samples. Data are available via SWATHAtlas (PASS01441).
format Online
Article
Text
id pubmed-7099061
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-70990612020-04-06 Generation of a murine SWATH-MS spectral library to quantify more than 11,000 proteins Zhong, Chuan-Qi Wu, Jianfeng Qiu, Xingfeng Chen, Xi Xie, Changchuan Han, Jiahuai Sci Data Data Descriptor Targeted SWATH-MS data analysis is critically dependent on the spectral library. Comprehensive spectral libraries of human or several other organisms have been published, but the extensive spectral library for mouse, a widely used model organism is not available. Here, we present a large murine spectral library covering more than 11,000 proteins and 240,000 proteotypic peptides, which included proteins derived from 9 murine tissue samples and one murine L929 cell line. This resource supports the quantification of 67% of all murine proteins annotated by UniProtKB/Swiss-Prot. Furthermore, we applied the spectral library to SWATH-MS data from murine tissue samples. Data are available via SWATHAtlas (PASS01441). Nature Publishing Group UK 2020-03-26 /pmc/articles/PMC7099061/ /pubmed/32218446 http://dx.doi.org/10.1038/s41597-020-0449-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files associated with this article.
spellingShingle Data Descriptor
Zhong, Chuan-Qi
Wu, Jianfeng
Qiu, Xingfeng
Chen, Xi
Xie, Changchuan
Han, Jiahuai
Generation of a murine SWATH-MS spectral library to quantify more than 11,000 proteins
title Generation of a murine SWATH-MS spectral library to quantify more than 11,000 proteins
title_full Generation of a murine SWATH-MS spectral library to quantify more than 11,000 proteins
title_fullStr Generation of a murine SWATH-MS spectral library to quantify more than 11,000 proteins
title_full_unstemmed Generation of a murine SWATH-MS spectral library to quantify more than 11,000 proteins
title_short Generation of a murine SWATH-MS spectral library to quantify more than 11,000 proteins
title_sort generation of a murine swath-ms spectral library to quantify more than 11,000 proteins
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7099061/
https://www.ncbi.nlm.nih.gov/pubmed/32218446
http://dx.doi.org/10.1038/s41597-020-0449-z
work_keys_str_mv AT zhongchuanqi generationofamurineswathmsspectrallibrarytoquantifymorethan11000proteins
AT wujianfeng generationofamurineswathmsspectrallibrarytoquantifymorethan11000proteins
AT qiuxingfeng generationofamurineswathmsspectrallibrarytoquantifymorethan11000proteins
AT chenxi generationofamurineswathmsspectrallibrarytoquantifymorethan11000proteins
AT xiechangchuan generationofamurineswathmsspectrallibrarytoquantifymorethan11000proteins
AT hanjiahuai generationofamurineswathmsspectrallibrarytoquantifymorethan11000proteins

Ejemplares similares