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Rapid and Real-time Detection of Human Viral Infections: Current Trends and Future Perspectives
The development of technologies with rapid and sensitive detection capabilities and increased throughput have become crucial for responding to greater number of threats posed by emerging and re-emerging viruses in the recent past. The conventional identification methods require time-consuming cultur...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7099323/ https://www.ncbi.nlm.nih.gov/pubmed/32226203 http://dx.doi.org/10.1007/s40011-011-0015-8 |
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author | Parida, Manmohan Shukla, Jyoti Sharma, Shashi Lakshmana Rao, P. V. |
author_facet | Parida, Manmohan Shukla, Jyoti Sharma, Shashi Lakshmana Rao, P. V. |
author_sort | Parida, Manmohan |
collection | PubMed |
description | The development of technologies with rapid and sensitive detection capabilities and increased throughput have become crucial for responding to greater number of threats posed by emerging and re-emerging viruses in the recent past. The conventional identification methods require time-consuming culturing, and/or detection of antibodies, which are not very sensitive and specific. The recent advances in molecular biology techniques in the field of genomics and proteomics greatly facilitate the rapid identification with more accuracy. The real-time assays viz; SYBR green I based real time RT-PCR and RT-LAMP have been developed for rapid detection as well as typing of some of the emerging arboviruses of biomedical importance viz; Dengue, Japanese Encephalitis, Chikungunya, West Nile, SARS and Swine Flu etc. Both these techniques are capable of detection and differentiation as well as quantifying viral load with higher sensitivity, rapidity and specificity. One of the most important advantages of RT-LAMP is its field applicability, without requirement of any sophisticated equipments. The establishment of these real time molecular assays will certainly facilitate the rapid detection of viruses with high degree of precision and accuracy in future. |
format | Online Article Text |
id | pubmed-7099323 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-70993232020-03-27 Rapid and Real-time Detection of Human Viral Infections: Current Trends and Future Perspectives Parida, Manmohan Shukla, Jyoti Sharma, Shashi Lakshmana Rao, P. V. Proc Natl Acad Sci India Sect B Biol Sci Review The development of technologies with rapid and sensitive detection capabilities and increased throughput have become crucial for responding to greater number of threats posed by emerging and re-emerging viruses in the recent past. The conventional identification methods require time-consuming culturing, and/or detection of antibodies, which are not very sensitive and specific. The recent advances in molecular biology techniques in the field of genomics and proteomics greatly facilitate the rapid identification with more accuracy. The real-time assays viz; SYBR green I based real time RT-PCR and RT-LAMP have been developed for rapid detection as well as typing of some of the emerging arboviruses of biomedical importance viz; Dengue, Japanese Encephalitis, Chikungunya, West Nile, SARS and Swine Flu etc. Both these techniques are capable of detection and differentiation as well as quantifying viral load with higher sensitivity, rapidity and specificity. One of the most important advantages of RT-LAMP is its field applicability, without requirement of any sophisticated equipments. The establishment of these real time molecular assays will certainly facilitate the rapid detection of viruses with high degree of precision and accuracy in future. Springer-Verlag 2012-01-18 2012 /pmc/articles/PMC7099323/ /pubmed/32226203 http://dx.doi.org/10.1007/s40011-011-0015-8 Text en © The National Academy of Sciences, India 2012 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Review Parida, Manmohan Shukla, Jyoti Sharma, Shashi Lakshmana Rao, P. V. Rapid and Real-time Detection of Human Viral Infections: Current Trends and Future Perspectives |
title | Rapid and Real-time Detection of Human Viral Infections: Current Trends and Future Perspectives |
title_full | Rapid and Real-time Detection of Human Viral Infections: Current Trends and Future Perspectives |
title_fullStr | Rapid and Real-time Detection of Human Viral Infections: Current Trends and Future Perspectives |
title_full_unstemmed | Rapid and Real-time Detection of Human Viral Infections: Current Trends and Future Perspectives |
title_short | Rapid and Real-time Detection of Human Viral Infections: Current Trends and Future Perspectives |
title_sort | rapid and real-time detection of human viral infections: current trends and future perspectives |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7099323/ https://www.ncbi.nlm.nih.gov/pubmed/32226203 http://dx.doi.org/10.1007/s40011-011-0015-8 |
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