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Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance

BACKGROUND: Reverse transcriptase is an indispensable enzyme for real-time reverse transcriptase (RT)-PCR, a standard method in molecular diagnostics for detection and quantification of defined RNA molecules. The prevention of non-specific products due to elongation of misprimed oligonucleotides by...

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Detalles Bibliográficos
Autores principales:	Rutschke, Nils, Zimmermann, Jan, Möller, Ronny, Klöck, Gerd, Winterhalter, Mathias, Leune, Annika
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Korea Basic Science Institute 2015
Materias:	Short Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7099353/ https://www.ncbi.nlm.nih.gov/pubmed/32226638 http://dx.doi.org/10.1186/s40543-015-0063-4

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