Cargando…

Comparative profiling of skeletal muscle models reveals heterogeneity of transcriptome and metabolism

Rat L6, mouse C2C12, and primary human skeletal muscle cells (HSMCs) are commonly used to study biological processes in skeletal muscle, and experimental data on these models are abundant. However, consistently matched experimental data are scarce, and comparisons between the different cell types an...

Descripción completa

Detalles Bibliográficos
Autores principales: Abdelmoez, Ahmed M., Sardón Puig, Laura, Smith, Jonathon A. B., Gabriel, Brendan M., Savikj, Mladen, Dollet, Lucile, Chibalin, Alexander V., Krook, Anna, Zierath, Juleen R., Pillon, Nicolas J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Physiological Society 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7099524/
https://www.ncbi.nlm.nih.gov/pubmed/31825657
http://dx.doi.org/10.1152/ajpcell.00540.2019
_version_ 1783511322788364288
author Abdelmoez, Ahmed M.
Sardón Puig, Laura
Smith, Jonathon A. B.
Gabriel, Brendan M.
Savikj, Mladen
Dollet, Lucile
Chibalin, Alexander V.
Krook, Anna
Zierath, Juleen R.
Pillon, Nicolas J.
author_facet Abdelmoez, Ahmed M.
Sardón Puig, Laura
Smith, Jonathon A. B.
Gabriel, Brendan M.
Savikj, Mladen
Dollet, Lucile
Chibalin, Alexander V.
Krook, Anna
Zierath, Juleen R.
Pillon, Nicolas J.
author_sort Abdelmoez, Ahmed M.
collection PubMed
description Rat L6, mouse C2C12, and primary human skeletal muscle cells (HSMCs) are commonly used to study biological processes in skeletal muscle, and experimental data on these models are abundant. However, consistently matched experimental data are scarce, and comparisons between the different cell types and adult tissue are problematic. We hypothesized that metabolic differences between these cellular models may be reflected at the mRNA level. Publicly available data sets were used to profile mRNA levels in myotubes and skeletal muscle tissues. L6, C2C12, and HSMC myotubes were assessed for proliferation, glucose uptake, glycogen synthesis, mitochondrial activity, and substrate oxidation, as well as the response to in vitro contraction. Transcriptomic profiling revealed that mRNA of genes coding for actin and myosin was enriched in C2C12, whereas L6 myotubes had the highest levels of genes encoding glucose transporters and the five complexes of the mitochondrial electron transport chain. Consistently, insulin-stimulated glucose uptake and oxidative capacity were greatest in L6 myotubes. Insulin-induced glycogen synthesis was highest in HSMCs, but C2C12 myotubes had higher baseline glucose oxidation. All models responded to electrical pulse stimulation-induced glucose uptake and gene expression but in a slightly different manner. Our analysis reveals a great degree of heterogeneity in the transcriptomic and metabolic profiles of L6, C2C12, or primary human myotubes. Based on these distinct signatures, we provide recommendations for the appropriate use of these models depending on scientific hypotheses and biological relevance.
format Online
Article
Text
id pubmed-7099524
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher American Physiological Society
record_format MEDLINE/PubMed
spelling pubmed-70995242020-04-02 Comparative profiling of skeletal muscle models reveals heterogeneity of transcriptome and metabolism Abdelmoez, Ahmed M. Sardón Puig, Laura Smith, Jonathon A. B. Gabriel, Brendan M. Savikj, Mladen Dollet, Lucile Chibalin, Alexander V. Krook, Anna Zierath, Juleen R. Pillon, Nicolas J. Am J Physiol Cell Physiol Research Article Rat L6, mouse C2C12, and primary human skeletal muscle cells (HSMCs) are commonly used to study biological processes in skeletal muscle, and experimental data on these models are abundant. However, consistently matched experimental data are scarce, and comparisons between the different cell types and adult tissue are problematic. We hypothesized that metabolic differences between these cellular models may be reflected at the mRNA level. Publicly available data sets were used to profile mRNA levels in myotubes and skeletal muscle tissues. L6, C2C12, and HSMC myotubes were assessed for proliferation, glucose uptake, glycogen synthesis, mitochondrial activity, and substrate oxidation, as well as the response to in vitro contraction. Transcriptomic profiling revealed that mRNA of genes coding for actin and myosin was enriched in C2C12, whereas L6 myotubes had the highest levels of genes encoding glucose transporters and the five complexes of the mitochondrial electron transport chain. Consistently, insulin-stimulated glucose uptake and oxidative capacity were greatest in L6 myotubes. Insulin-induced glycogen synthesis was highest in HSMCs, but C2C12 myotubes had higher baseline glucose oxidation. All models responded to electrical pulse stimulation-induced glucose uptake and gene expression but in a slightly different manner. Our analysis reveals a great degree of heterogeneity in the transcriptomic and metabolic profiles of L6, C2C12, or primary human myotubes. Based on these distinct signatures, we provide recommendations for the appropriate use of these models depending on scientific hypotheses and biological relevance. American Physiological Society 2020-03-01 2019-12-11 /pmc/articles/PMC7099524/ /pubmed/31825657 http://dx.doi.org/10.1152/ajpcell.00540.2019 Text en Copyright © 2020 the American Physiological Society http://creativecommons.org/licenses/by/4.0/deed.en_US Licensed under Creative Commons Attribution CC-BY 4.0 (http://creativecommons.org/licenses/by/4.0/deed.en_US) : © the American Physiological Society.
spellingShingle Research Article
Abdelmoez, Ahmed M.
Sardón Puig, Laura
Smith, Jonathon A. B.
Gabriel, Brendan M.
Savikj, Mladen
Dollet, Lucile
Chibalin, Alexander V.
Krook, Anna
Zierath, Juleen R.
Pillon, Nicolas J.
Comparative profiling of skeletal muscle models reveals heterogeneity of transcriptome and metabolism
title Comparative profiling of skeletal muscle models reveals heterogeneity of transcriptome and metabolism
title_full Comparative profiling of skeletal muscle models reveals heterogeneity of transcriptome and metabolism
title_fullStr Comparative profiling of skeletal muscle models reveals heterogeneity of transcriptome and metabolism
title_full_unstemmed Comparative profiling of skeletal muscle models reveals heterogeneity of transcriptome and metabolism
title_short Comparative profiling of skeletal muscle models reveals heterogeneity of transcriptome and metabolism
title_sort comparative profiling of skeletal muscle models reveals heterogeneity of transcriptome and metabolism
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7099524/
https://www.ncbi.nlm.nih.gov/pubmed/31825657
http://dx.doi.org/10.1152/ajpcell.00540.2019
work_keys_str_mv AT abdelmoezahmedm comparativeprofilingofskeletalmusclemodelsrevealsheterogeneityoftranscriptomeandmetabolism
AT sardonpuiglaura comparativeprofilingofskeletalmusclemodelsrevealsheterogeneityoftranscriptomeandmetabolism
AT smithjonathonab comparativeprofilingofskeletalmusclemodelsrevealsheterogeneityoftranscriptomeandmetabolism
AT gabrielbrendanm comparativeprofilingofskeletalmusclemodelsrevealsheterogeneityoftranscriptomeandmetabolism
AT savikjmladen comparativeprofilingofskeletalmusclemodelsrevealsheterogeneityoftranscriptomeandmetabolism
AT dolletlucile comparativeprofilingofskeletalmusclemodelsrevealsheterogeneityoftranscriptomeandmetabolism
AT chibalinalexanderv comparativeprofilingofskeletalmusclemodelsrevealsheterogeneityoftranscriptomeandmetabolism
AT krookanna comparativeprofilingofskeletalmusclemodelsrevealsheterogeneityoftranscriptomeandmetabolism
AT zierathjuleenr comparativeprofilingofskeletalmusclemodelsrevealsheterogeneityoftranscriptomeandmetabolism
AT pillonnicolasj comparativeprofilingofskeletalmusclemodelsrevealsheterogeneityoftranscriptomeandmetabolism