Cargando…
Chondrogenic differentiation and three dimensional chondrogenesis of human adipose-derived stem cells induced by engineered cartilage-derived conditional media
Due to lack of optimal inductive protocols, how to effectively improve chondrogenesis of adiposederived stem cells (ASCs) is still a great challenge. Our previous studies demonstrated that the culture media derived from chondrocyte-scaffold constructs (conditional media) contained various soluble ch...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7100146/ http://dx.doi.org/10.1007/s13770-013-1120-y |
_version_ | 1783511415614603264 |
---|---|
author | Sun, Hengyun Liu, Yu Jiang, Ting Liu, Xia He, Aijuan Li, Jie Zhang, Wenjie Liu, Wei Cao, Yilin Zhou, Guangdong |
author_facet | Sun, Hengyun Liu, Yu Jiang, Ting Liu, Xia He, Aijuan Li, Jie Zhang, Wenjie Liu, Wei Cao, Yilin Zhou, Guangdong |
author_sort | Sun, Hengyun |
collection | PubMed |
description | Due to lack of optimal inductive protocols, how to effectively improve chondrogenesis of adiposederived stem cells (ASCs) is still a great challenge. Our previous studies demonstrated that the culture media derived from chondrocyte-scaffold constructs (conditional media) contained various soluble chondrogenic factors and were effective for directing chondrogenic differentiation of bone marrow stem cells. Nevertheless, it remains unclear whether the conditional media can induce ASCs towards chondrogenic differentiation, especially for three-dimensional (3D) cartilage formation in a preshaped scaffold. In this study, it demonstrated that the conditional media derived from chondrocyte-scaffold constructs could promote ASCs to differentiate into chondrocyte-like cells, with similar expression of type II collagen to those induced by chondrogenic growth factors. Moreover, the expression level of chondrocyte-specific genes, such as SOX9, type II collagen, and COMP, was even higher in conditional medium group (CM) than that in optimized chondrogenic growth factor group (GF), indicating that the conditional media can serve as an effective inducer for chondrogenic differentiation of ASCs. Most importantly, the conditional media could also induce ASC-scaffold constructs to form 3D cartilage-like tissue with typical lacunae structures and positive expression of cartilage specific matrices, even higher contents of GAG and type II collagen were achieved in CM group compared to GF group. The current study establishes a simple, but stable, efficient, and economical method for directing 3D cartilage formation of ASCs, a strategy that may be more closely applicable for repairing cartilage defects. |
format | Online Article Text |
id | pubmed-7100146 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-71001462020-03-27 Chondrogenic differentiation and three dimensional chondrogenesis of human adipose-derived stem cells induced by engineered cartilage-derived conditional media Sun, Hengyun Liu, Yu Jiang, Ting Liu, Xia He, Aijuan Li, Jie Zhang, Wenjie Liu, Wei Cao, Yilin Zhou, Guangdong Tissue Eng Regen Med Original Article Due to lack of optimal inductive protocols, how to effectively improve chondrogenesis of adiposederived stem cells (ASCs) is still a great challenge. Our previous studies demonstrated that the culture media derived from chondrocyte-scaffold constructs (conditional media) contained various soluble chondrogenic factors and were effective for directing chondrogenic differentiation of bone marrow stem cells. Nevertheless, it remains unclear whether the conditional media can induce ASCs towards chondrogenic differentiation, especially for three-dimensional (3D) cartilage formation in a preshaped scaffold. In this study, it demonstrated that the conditional media derived from chondrocyte-scaffold constructs could promote ASCs to differentiate into chondrocyte-like cells, with similar expression of type II collagen to those induced by chondrogenic growth factors. Moreover, the expression level of chondrocyte-specific genes, such as SOX9, type II collagen, and COMP, was even higher in conditional medium group (CM) than that in optimized chondrogenic growth factor group (GF), indicating that the conditional media can serve as an effective inducer for chondrogenic differentiation of ASCs. Most importantly, the conditional media could also induce ASC-scaffold constructs to form 3D cartilage-like tissue with typical lacunae structures and positive expression of cartilage specific matrices, even higher contents of GAG and type II collagen were achieved in CM group compared to GF group. The current study establishes a simple, but stable, efficient, and economical method for directing 3D cartilage formation of ASCs, a strategy that may be more closely applicable for repairing cartilage defects. Springer Netherlands 2014-01-25 /pmc/articles/PMC7100146/ http://dx.doi.org/10.1007/s13770-013-1120-y Text en © The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht 2014 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Sun, Hengyun Liu, Yu Jiang, Ting Liu, Xia He, Aijuan Li, Jie Zhang, Wenjie Liu, Wei Cao, Yilin Zhou, Guangdong Chondrogenic differentiation and three dimensional chondrogenesis of human adipose-derived stem cells induced by engineered cartilage-derived conditional media |
title | Chondrogenic differentiation and three dimensional chondrogenesis of human adipose-derived stem cells induced by engineered cartilage-derived conditional media |
title_full | Chondrogenic differentiation and three dimensional chondrogenesis of human adipose-derived stem cells induced by engineered cartilage-derived conditional media |
title_fullStr | Chondrogenic differentiation and three dimensional chondrogenesis of human adipose-derived stem cells induced by engineered cartilage-derived conditional media |
title_full_unstemmed | Chondrogenic differentiation and three dimensional chondrogenesis of human adipose-derived stem cells induced by engineered cartilage-derived conditional media |
title_short | Chondrogenic differentiation and three dimensional chondrogenesis of human adipose-derived stem cells induced by engineered cartilage-derived conditional media |
title_sort | chondrogenic differentiation and three dimensional chondrogenesis of human adipose-derived stem cells induced by engineered cartilage-derived conditional media |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7100146/ http://dx.doi.org/10.1007/s13770-013-1120-y |
work_keys_str_mv | AT sunhengyun chondrogenicdifferentiationandthreedimensionalchondrogenesisofhumanadiposederivedstemcellsinducedbyengineeredcartilagederivedconditionalmedia AT liuyu chondrogenicdifferentiationandthreedimensionalchondrogenesisofhumanadiposederivedstemcellsinducedbyengineeredcartilagederivedconditionalmedia AT jiangting chondrogenicdifferentiationandthreedimensionalchondrogenesisofhumanadiposederivedstemcellsinducedbyengineeredcartilagederivedconditionalmedia AT liuxia chondrogenicdifferentiationandthreedimensionalchondrogenesisofhumanadiposederivedstemcellsinducedbyengineeredcartilagederivedconditionalmedia AT heaijuan chondrogenicdifferentiationandthreedimensionalchondrogenesisofhumanadiposederivedstemcellsinducedbyengineeredcartilagederivedconditionalmedia AT lijie chondrogenicdifferentiationandthreedimensionalchondrogenesisofhumanadiposederivedstemcellsinducedbyengineeredcartilagederivedconditionalmedia AT zhangwenjie chondrogenicdifferentiationandthreedimensionalchondrogenesisofhumanadiposederivedstemcellsinducedbyengineeredcartilagederivedconditionalmedia AT liuwei chondrogenicdifferentiationandthreedimensionalchondrogenesisofhumanadiposederivedstemcellsinducedbyengineeredcartilagederivedconditionalmedia AT caoyilin chondrogenicdifferentiationandthreedimensionalchondrogenesisofhumanadiposederivedstemcellsinducedbyengineeredcartilagederivedconditionalmedia AT zhouguangdong chondrogenicdifferentiationandthreedimensionalchondrogenesisofhumanadiposederivedstemcellsinducedbyengineeredcartilagederivedconditionalmedia |