Novel Rapid Test for Detecting Carbapenemase

We developed a carbapenemase test based on the ability of imipenem to inhibit noncarbapenemase β-lactamases. The test uses bacterial isolates with a fluorescent β-lactamase substrate, producing objective results with 100% sensitivity and specificity in 10 minutes. The assay is inexpensive and consis...

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Detalles Bibliográficos
Autores principales: Feng, Yanfang, Palanisami, Akilan, Kuriakose, Jerrin, Pigula, Michael, Ashraf, Shoaib, Hasan, Tayyaba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2020
Materias:
Research Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101118/
https://www.ncbi.nlm.nih.gov/pubmed/32186503
http://dx.doi.org/10.3201/eid2604.181655
Descripción
Sumario:We developed a carbapenemase test based on the ability of imipenem to inhibit noncarbapenemase β-lactamases. The test uses bacterial isolates with a fluorescent β-lactamase substrate, producing objective results with 100% sensitivity and specificity in 10 minutes. The assay is inexpensive and consists of only 1 mixing step.

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