Novel Rapid Test for Detecting Carbapenemase

We developed a carbapenemase test based on the ability of imipenem to inhibit noncarbapenemase β-lactamases. The test uses bacterial isolates with a fluorescent β-lactamase substrate, producing objective results with 100% sensitivity and specificity in 10 minutes. The assay is inexpensive and consis...

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Detalles Bibliográficos
Autores principales: Feng, Yanfang, Palanisami, Akilan, Kuriakose, Jerrin, Pigula, Michael, Ashraf, Shoaib, Hasan, Tayyaba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2020
Materias:
Research Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101118/
https://www.ncbi.nlm.nih.gov/pubmed/32186503
http://dx.doi.org/10.3201/eid2604.181655
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author Feng, Yanfang
Palanisami, Akilan
Kuriakose, Jerrin
Pigula, Michael
Ashraf, Shoaib
Hasan, Tayyaba
author_facet Feng, Yanfang
Palanisami, Akilan
Kuriakose, Jerrin
Pigula, Michael
Ashraf, Shoaib
Hasan, Tayyaba
author_sort Feng, Yanfang
collection PubMed
description We developed a carbapenemase test based on the ability of imipenem to inhibit noncarbapenemase β-lactamases. The test uses bacterial isolates with a fluorescent β-lactamase substrate, producing objective results with 100% sensitivity and specificity in 10 minutes. The assay is inexpensive and consists of only 1 mixing step.
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spelling pubmed-71011182020-04-03 Novel Rapid Test for Detecting Carbapenemase Feng, Yanfang Palanisami, Akilan Kuriakose, Jerrin Pigula, Michael Ashraf, Shoaib Hasan, Tayyaba Emerg Infect Dis Research Letter We developed a carbapenemase test based on the ability of imipenem to inhibit noncarbapenemase β-lactamases. The test uses bacterial isolates with a fluorescent β-lactamase substrate, producing objective results with 100% sensitivity and specificity in 10 minutes. The assay is inexpensive and consists of only 1 mixing step. Centers for Disease Control and Prevention 2020-04 /pmc/articles/PMC7101118/ /pubmed/32186503 http://dx.doi.org/10.3201/eid2604.181655 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research Letter
Feng, Yanfang
Palanisami, Akilan
Kuriakose, Jerrin
Pigula, Michael
Ashraf, Shoaib
Hasan, Tayyaba
Novel Rapid Test for Detecting Carbapenemase
title Novel Rapid Test for Detecting Carbapenemase
title_full Novel Rapid Test for Detecting Carbapenemase
title_fullStr Novel Rapid Test for Detecting Carbapenemase
title_full_unstemmed Novel Rapid Test for Detecting Carbapenemase
title_short Novel Rapid Test for Detecting Carbapenemase
title_sort novel rapid test for detecting carbapenemase
topic Research Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101118/
https://www.ncbi.nlm.nih.gov/pubmed/32186503
http://dx.doi.org/10.3201/eid2604.181655
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AT pigulamichael novelrapidtestfordetectingcarbapenemase
AT ashrafshoaib novelrapidtestfordetectingcarbapenemase
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