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Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium

Gallibacterium anatis is an opportunistic pathogen, previously associated with deaths in poultry, domestic birds, and occasionally humans. We obtained G. anatis isolates from bronchoalveolar lavage samples of 10 calves with bronchopneumonia unresponsive to antimicrobial therapy. Collected isolates w...

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Autores principales: Van Driessche, Laura, Vanneste, Kevin, Bogaerts, Bert, De Keersmaecker, Sigrid C.J., Roosens, Nancy H., Haesebrouck, Freddy, De Cremer, Lieze, Deprez, Piet, Pardon, Bart, Boyen, Filip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101127/
https://www.ncbi.nlm.nih.gov/pubmed/32186507
http://dx.doi.org/10.3201/eid2604.190962
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author Van Driessche, Laura
Vanneste, Kevin
Bogaerts, Bert
De Keersmaecker, Sigrid C.J.
Roosens, Nancy H.
Haesebrouck, Freddy
De Cremer, Lieze
Deprez, Piet
Pardon, Bart
Boyen, Filip
author_facet Van Driessche, Laura
Vanneste, Kevin
Bogaerts, Bert
De Keersmaecker, Sigrid C.J.
Roosens, Nancy H.
Haesebrouck, Freddy
De Cremer, Lieze
Deprez, Piet
Pardon, Bart
Boyen, Filip
author_sort Van Driessche, Laura
collection PubMed
description Gallibacterium anatis is an opportunistic pathogen, previously associated with deaths in poultry, domestic birds, and occasionally humans. We obtained G. anatis isolates from bronchoalveolar lavage samples of 10 calves with bronchopneumonia unresponsive to antimicrobial therapy. Collected isolates were multidrug-resistant to extensively drug-resistant, exhibiting resistance against 5–7 classes of antimicrobial drugs. Whole-genome sequencing revealed 24 different antimicrobial-resistance determinants, including genes not previously described in the Gallibacterium genus or even the Pasteurellaceae family, such as aadA23, bla(CARB-8), tet(Y), and qnrD1. Some resistance genes were closely linked in resistance gene cassettes with either transposases in close proximity or situated on putative mobile elements or predicted plasmids. Single-nucleotide polymorphism genotyping revealed large genetic variation between the G. anatis isolates, including isolates retrieved from the same farm. G. anatis might play a hitherto unrecognized role as a respiratory pathogen and resistance gene reservoir in cattle and has unknown zoonotic potential.
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spelling pubmed-71011272020-04-03 Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium Van Driessche, Laura Vanneste, Kevin Bogaerts, Bert De Keersmaecker, Sigrid C.J. Roosens, Nancy H. Haesebrouck, Freddy De Cremer, Lieze Deprez, Piet Pardon, Bart Boyen, Filip Emerg Infect Dis Research Gallibacterium anatis is an opportunistic pathogen, previously associated with deaths in poultry, domestic birds, and occasionally humans. We obtained G. anatis isolates from bronchoalveolar lavage samples of 10 calves with bronchopneumonia unresponsive to antimicrobial therapy. Collected isolates were multidrug-resistant to extensively drug-resistant, exhibiting resistance against 5–7 classes of antimicrobial drugs. Whole-genome sequencing revealed 24 different antimicrobial-resistance determinants, including genes not previously described in the Gallibacterium genus or even the Pasteurellaceae family, such as aadA23, bla(CARB-8), tet(Y), and qnrD1. Some resistance genes were closely linked in resistance gene cassettes with either transposases in close proximity or situated on putative mobile elements or predicted plasmids. Single-nucleotide polymorphism genotyping revealed large genetic variation between the G. anatis isolates, including isolates retrieved from the same farm. G. anatis might play a hitherto unrecognized role as a respiratory pathogen and resistance gene reservoir in cattle and has unknown zoonotic potential. Centers for Disease Control and Prevention 2020-04 /pmc/articles/PMC7101127/ /pubmed/32186507 http://dx.doi.org/10.3201/eid2604.190962 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Van Driessche, Laura
Vanneste, Kevin
Bogaerts, Bert
De Keersmaecker, Sigrid C.J.
Roosens, Nancy H.
Haesebrouck, Freddy
De Cremer, Lieze
Deprez, Piet
Pardon, Bart
Boyen, Filip
Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium
title Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium
title_full Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium
title_fullStr Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium
title_full_unstemmed Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium
title_short Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium
title_sort isolation of drug-resistant gallibacterium anatis from calves with unresponsive bronchopneumonia, belgium
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101127/
https://www.ncbi.nlm.nih.gov/pubmed/32186507
http://dx.doi.org/10.3201/eid2604.190962
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