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Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium
Gallibacterium anatis is an opportunistic pathogen, previously associated with deaths in poultry, domestic birds, and occasionally humans. We obtained G. anatis isolates from bronchoalveolar lavage samples of 10 calves with bronchopneumonia unresponsive to antimicrobial therapy. Collected isolates w...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Centers for Disease Control and Prevention
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101127/ https://www.ncbi.nlm.nih.gov/pubmed/32186507 http://dx.doi.org/10.3201/eid2604.190962 |
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author | Van Driessche, Laura Vanneste, Kevin Bogaerts, Bert De Keersmaecker, Sigrid C.J. Roosens, Nancy H. Haesebrouck, Freddy De Cremer, Lieze Deprez, Piet Pardon, Bart Boyen, Filip |
author_facet | Van Driessche, Laura Vanneste, Kevin Bogaerts, Bert De Keersmaecker, Sigrid C.J. Roosens, Nancy H. Haesebrouck, Freddy De Cremer, Lieze Deprez, Piet Pardon, Bart Boyen, Filip |
author_sort | Van Driessche, Laura |
collection | PubMed |
description | Gallibacterium anatis is an opportunistic pathogen, previously associated with deaths in poultry, domestic birds, and occasionally humans. We obtained G. anatis isolates from bronchoalveolar lavage samples of 10 calves with bronchopneumonia unresponsive to antimicrobial therapy. Collected isolates were multidrug-resistant to extensively drug-resistant, exhibiting resistance against 5–7 classes of antimicrobial drugs. Whole-genome sequencing revealed 24 different antimicrobial-resistance determinants, including genes not previously described in the Gallibacterium genus or even the Pasteurellaceae family, such as aadA23, bla(CARB-8), tet(Y), and qnrD1. Some resistance genes were closely linked in resistance gene cassettes with either transposases in close proximity or situated on putative mobile elements or predicted plasmids. Single-nucleotide polymorphism genotyping revealed large genetic variation between the G. anatis isolates, including isolates retrieved from the same farm. G. anatis might play a hitherto unrecognized role as a respiratory pathogen and resistance gene reservoir in cattle and has unknown zoonotic potential. |
format | Online Article Text |
id | pubmed-7101127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Centers for Disease Control and Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-71011272020-04-03 Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium Van Driessche, Laura Vanneste, Kevin Bogaerts, Bert De Keersmaecker, Sigrid C.J. Roosens, Nancy H. Haesebrouck, Freddy De Cremer, Lieze Deprez, Piet Pardon, Bart Boyen, Filip Emerg Infect Dis Research Gallibacterium anatis is an opportunistic pathogen, previously associated with deaths in poultry, domestic birds, and occasionally humans. We obtained G. anatis isolates from bronchoalveolar lavage samples of 10 calves with bronchopneumonia unresponsive to antimicrobial therapy. Collected isolates were multidrug-resistant to extensively drug-resistant, exhibiting resistance against 5–7 classes of antimicrobial drugs. Whole-genome sequencing revealed 24 different antimicrobial-resistance determinants, including genes not previously described in the Gallibacterium genus or even the Pasteurellaceae family, such as aadA23, bla(CARB-8), tet(Y), and qnrD1. Some resistance genes were closely linked in resistance gene cassettes with either transposases in close proximity or situated on putative mobile elements or predicted plasmids. Single-nucleotide polymorphism genotyping revealed large genetic variation between the G. anatis isolates, including isolates retrieved from the same farm. G. anatis might play a hitherto unrecognized role as a respiratory pathogen and resistance gene reservoir in cattle and has unknown zoonotic potential. Centers for Disease Control and Prevention 2020-04 /pmc/articles/PMC7101127/ /pubmed/32186507 http://dx.doi.org/10.3201/eid2604.190962 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited. |
spellingShingle | Research Van Driessche, Laura Vanneste, Kevin Bogaerts, Bert De Keersmaecker, Sigrid C.J. Roosens, Nancy H. Haesebrouck, Freddy De Cremer, Lieze Deprez, Piet Pardon, Bart Boyen, Filip Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium |
title | Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium |
title_full | Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium |
title_fullStr | Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium |
title_full_unstemmed | Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium |
title_short | Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium |
title_sort | isolation of drug-resistant gallibacterium anatis from calves with unresponsive bronchopneumonia, belgium |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101127/ https://www.ncbi.nlm.nih.gov/pubmed/32186507 http://dx.doi.org/10.3201/eid2604.190962 |
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