In situ characterization of nanoscale strains in loaded whole joints via synchrotron X-ray tomography

Imaging techniques for quantifying how the hierarchical structure of deforming joints changes are constrained by destructive sample treatments, sample-size restrictions and lengthy scan times. Here, we report the use of fast, low-dose pink-beam synchrotron X-ray tomography combined with mechanical loading at nanometric precision for the in situ imaging, at resolutions lower than 100 nm, of mechanical strain in intact untreated joints under physiologically realistic conditions. We show that, in young, aged, and osteoarthritic mice, hierarchical changes in tissue structure and mechanical behaviour can be simultaneously visualized, and that tissue structure at the cellular level correlates with whole-joint mechanical performance. We also used the tomographic approach to study the co-localization of tissue strains to specific chondrocyte lacunar organizations within intact loaded joints, and for the exploration of the role of calcified-cartilage stiffness on the biomechanics of healthy and pathological joints.