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Avocado-derived polyols for use as novel co-surfactants in low energy self-emulsifying microemulsions
Avocado (Persea americana Mill.; Lauraceae) seed-derived polyhydroxylated fatty alcohols (PFAs) or polyols (i.e., avocadene and avocadyne) are metabolic modulators that selectively induce apoptosis of leukemia stem cells and reverse pathologies associated with diet-induced obesity. Delivery systems...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101315/ https://www.ncbi.nlm.nih.gov/pubmed/32221368 http://dx.doi.org/10.1038/s41598-020-62334-y |
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author | Ahmed, Nawaz Kermanshahi, Behnoush Ghazani, Saeed M. Tait, Katrina Tcheng, Matthew Roma, Alessia Callender, Shannon P. Smith, Richard W. Tam, William Wettig, Shawn D. Rogers, Michael A. Marangoni, Alejandro G. Spagnuolo, Paul A. |
author_facet | Ahmed, Nawaz Kermanshahi, Behnoush Ghazani, Saeed M. Tait, Katrina Tcheng, Matthew Roma, Alessia Callender, Shannon P. Smith, Richard W. Tam, William Wettig, Shawn D. Rogers, Michael A. Marangoni, Alejandro G. Spagnuolo, Paul A. |
author_sort | Ahmed, Nawaz |
collection | PubMed |
description | Avocado (Persea americana Mill.; Lauraceae) seed-derived polyhydroxylated fatty alcohols (PFAs) or polyols (i.e., avocadene and avocadyne) are metabolic modulators that selectively induce apoptosis of leukemia stem cells and reverse pathologies associated with diet-induced obesity. Delivery systems containing avocado polyols have not been described. Herein, natural surface active properties of these polyols are characterized and incorporated into self-emulsifying drug delivery systems (SEDDS) that rely on molecular self-assembly to form fine, transparent, oil-in-water (O/W) microemulsions as small as 20 nanometers in diameter. Mechanistically, a 1:1 molar ratio of avocadene and avocadyne (i.e., avocatin B or AVO was shown to be a eutectic mixture which can be employed as a novel, bioactive, co-surfactant that significantly reduces droplet size of medium-chain triglyceride O/W emulsions stabilized with polysorbate 80. In vitro cytotoxicity of avocado polyol-SEDDS in acute myeloid leukemia cell lines indicated significant increases in potency and bioactivity compared to conventional cell culture delivery systems. A pilot pharmacokinetic evaluation of AVO SEDDS in C57BL/6J mice revealed appreciable accumulation in whole blood and biodistribution in key target tissues. Lastly, incorporation of AVO in SEDDS significantly improved encapsulation of the poorly water-soluble drugs naproxen and curcumin. |
format | Online Article Text |
id | pubmed-7101315 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-71013152020-03-31 Avocado-derived polyols for use as novel co-surfactants in low energy self-emulsifying microemulsions Ahmed, Nawaz Kermanshahi, Behnoush Ghazani, Saeed M. Tait, Katrina Tcheng, Matthew Roma, Alessia Callender, Shannon P. Smith, Richard W. Tam, William Wettig, Shawn D. Rogers, Michael A. Marangoni, Alejandro G. Spagnuolo, Paul A. Sci Rep Article Avocado (Persea americana Mill.; Lauraceae) seed-derived polyhydroxylated fatty alcohols (PFAs) or polyols (i.e., avocadene and avocadyne) are metabolic modulators that selectively induce apoptosis of leukemia stem cells and reverse pathologies associated with diet-induced obesity. Delivery systems containing avocado polyols have not been described. Herein, natural surface active properties of these polyols are characterized and incorporated into self-emulsifying drug delivery systems (SEDDS) that rely on molecular self-assembly to form fine, transparent, oil-in-water (O/W) microemulsions as small as 20 nanometers in diameter. Mechanistically, a 1:1 molar ratio of avocadene and avocadyne (i.e., avocatin B or AVO was shown to be a eutectic mixture which can be employed as a novel, bioactive, co-surfactant that significantly reduces droplet size of medium-chain triglyceride O/W emulsions stabilized with polysorbate 80. In vitro cytotoxicity of avocado polyol-SEDDS in acute myeloid leukemia cell lines indicated significant increases in potency and bioactivity compared to conventional cell culture delivery systems. A pilot pharmacokinetic evaluation of AVO SEDDS in C57BL/6J mice revealed appreciable accumulation in whole blood and biodistribution in key target tissues. Lastly, incorporation of AVO in SEDDS significantly improved encapsulation of the poorly water-soluble drugs naproxen and curcumin. Nature Publishing Group UK 2020-03-27 /pmc/articles/PMC7101315/ /pubmed/32221368 http://dx.doi.org/10.1038/s41598-020-62334-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ahmed, Nawaz Kermanshahi, Behnoush Ghazani, Saeed M. Tait, Katrina Tcheng, Matthew Roma, Alessia Callender, Shannon P. Smith, Richard W. Tam, William Wettig, Shawn D. Rogers, Michael A. Marangoni, Alejandro G. Spagnuolo, Paul A. Avocado-derived polyols for use as novel co-surfactants in low energy self-emulsifying microemulsions |
title | Avocado-derived polyols for use as novel co-surfactants in low energy self-emulsifying microemulsions |
title_full | Avocado-derived polyols for use as novel co-surfactants in low energy self-emulsifying microemulsions |
title_fullStr | Avocado-derived polyols for use as novel co-surfactants in low energy self-emulsifying microemulsions |
title_full_unstemmed | Avocado-derived polyols for use as novel co-surfactants in low energy self-emulsifying microemulsions |
title_short | Avocado-derived polyols for use as novel co-surfactants in low energy self-emulsifying microemulsions |
title_sort | avocado-derived polyols for use as novel co-surfactants in low energy self-emulsifying microemulsions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101315/ https://www.ncbi.nlm.nih.gov/pubmed/32221368 http://dx.doi.org/10.1038/s41598-020-62334-y |
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