Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins

We propose the concept of universal fiducials based on a set of pre-made semi-synthetic antibodies (sABs) generated by customized phage display selections against the fusion protein BRIL, an engineered variant of apocytochrome b562a. These sABs can bind to BRIL fused either into the loops or termini...

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Detalles Bibliográficos
Autores principales: Mukherjee, Somnath, Erramilli, Satchal K., Ammirati, Mark, Alvarez, Frances J. D., Fennell, Kimberly F., Purdy, Michael D., Skrobek, Blazej M., Radziwon, Katarzyna, Coukos, John, Kang, Yanyong, Dutka, Przemysław, Gao, Xiang, Qiu, Xiayang, Yeager, Mark, Eric Xu, H., Han, Seungil, Kossiakoff, Anthony A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101349/
https://www.ncbi.nlm.nih.gov/pubmed/32221310
http://dx.doi.org/10.1038/s41467-020-15363-0

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