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Environmental stress perception activates structural remodeling of extant Streptococcus mutans biofilms

Transcription regulators from the LexA-like Protein Superfamily control a highly diverse assortment of genetic pathways in response to environmental stress. All characterized members of this family modulate their functionality and stability via a strict coordination with the coprotease function of R...

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Autores principales: Marx, Patrick, Sang, Yu, Qin, Hua, Wang, Qingjing, Guo, Rongkai, Pfeifer, Carmem, Kreth, Jens, Merritt, Justin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101444/
https://www.ncbi.nlm.nih.gov/pubmed/32221309
http://dx.doi.org/10.1038/s41522-020-0128-z
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author Marx, Patrick
Sang, Yu
Qin, Hua
Wang, Qingjing
Guo, Rongkai
Pfeifer, Carmem
Kreth, Jens
Merritt, Justin
author_facet Marx, Patrick
Sang, Yu
Qin, Hua
Wang, Qingjing
Guo, Rongkai
Pfeifer, Carmem
Kreth, Jens
Merritt, Justin
author_sort Marx, Patrick
collection PubMed
description Transcription regulators from the LexA-like Protein Superfamily control a highly diverse assortment of genetic pathways in response to environmental stress. All characterized members of this family modulate their functionality and stability via a strict coordination with the coprotease function of RecA. Using the LexA-like protein IrvR from Streptococcus mutans, we demonstrate an exception to the RecA paradigm and illustrate how this evolutionary innovation has been coopted to diversify the stress responsiveness of S. mutans biofilms. Using a combination of genetics and biophysical measurements, we demonstrate how non-SOS stresses and SOS stresses each trigger separate regulatory mechanisms that stimulate production of a surface lectin responsible for remodeling the viscoelastic properties of extant biofilms during episodes of environmental stress. These studies demonstrate how changes in the external environment or even anti-biofilm therapeutic agents can activate biofilm-specific adaptive mechanisms responsible for bolstering the integrity of established biofilm communities. Such changes in biofilm community structure are likely to play central roles in the notorious recalcitrance of biofilm infections.
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spelling pubmed-71014442020-04-06 Environmental stress perception activates structural remodeling of extant Streptococcus mutans biofilms Marx, Patrick Sang, Yu Qin, Hua Wang, Qingjing Guo, Rongkai Pfeifer, Carmem Kreth, Jens Merritt, Justin NPJ Biofilms Microbiomes Article Transcription regulators from the LexA-like Protein Superfamily control a highly diverse assortment of genetic pathways in response to environmental stress. All characterized members of this family modulate their functionality and stability via a strict coordination with the coprotease function of RecA. Using the LexA-like protein IrvR from Streptococcus mutans, we demonstrate an exception to the RecA paradigm and illustrate how this evolutionary innovation has been coopted to diversify the stress responsiveness of S. mutans biofilms. Using a combination of genetics and biophysical measurements, we demonstrate how non-SOS stresses and SOS stresses each trigger separate regulatory mechanisms that stimulate production of a surface lectin responsible for remodeling the viscoelastic properties of extant biofilms during episodes of environmental stress. These studies demonstrate how changes in the external environment or even anti-biofilm therapeutic agents can activate biofilm-specific adaptive mechanisms responsible for bolstering the integrity of established biofilm communities. Such changes in biofilm community structure are likely to play central roles in the notorious recalcitrance of biofilm infections. Nature Publishing Group UK 2020-03-27 /pmc/articles/PMC7101444/ /pubmed/32221309 http://dx.doi.org/10.1038/s41522-020-0128-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Marx, Patrick
Sang, Yu
Qin, Hua
Wang, Qingjing
Guo, Rongkai
Pfeifer, Carmem
Kreth, Jens
Merritt, Justin
Environmental stress perception activates structural remodeling of extant Streptococcus mutans biofilms
title Environmental stress perception activates structural remodeling of extant Streptococcus mutans biofilms
title_full Environmental stress perception activates structural remodeling of extant Streptococcus mutans biofilms
title_fullStr Environmental stress perception activates structural remodeling of extant Streptococcus mutans biofilms
title_full_unstemmed Environmental stress perception activates structural remodeling of extant Streptococcus mutans biofilms
title_short Environmental stress perception activates structural remodeling of extant Streptococcus mutans biofilms
title_sort environmental stress perception activates structural remodeling of extant streptococcus mutans biofilms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101444/
https://www.ncbi.nlm.nih.gov/pubmed/32221309
http://dx.doi.org/10.1038/s41522-020-0128-z
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