Studies on histoplasmosis IV. Elution characteristics of the Histo-inhibitory factor (HIF)

The histo-inhibitory factor (HIF) derived from homogenates of liver or kidney from hamsters infected withHistoplasma capsulatum has been fractionated by column chromatography. It shows maximum absorption at 280 mµ, has a molecular weight of 142,000 and can be eluted from DEAE-cellulose or DEAE-Sepha...

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Detalles Bibliográficos
Autores principales: O'Hern, E. M., Fishbein, L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kluwer Academic Publishers 1968
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101975/
https://www.ncbi.nlm.nih.gov/pubmed/5748624
http://dx.doi.org/10.1007/BF02057164
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author O'Hern, E. M.
Fishbein, L.
author_facet O'Hern, E. M.
Fishbein, L.
author_sort O'Hern, E. M.
collection PubMed
description The histo-inhibitory factor (HIF) derived from homogenates of liver or kidney from hamsters infected withHistoplasma capsulatum has been fractionated by column chromatography. It shows maximum absorption at 280 mµ, has a molecular weight of 142,000 and can be eluted from DEAE-cellulose or DEAE-Sephadex A—50 with 0.02 M phosphate — 4 M sodium chloride (1 : 1) HIF can be eluted fromHistoplasma yeast cells at pH 10.0, thus a greater number of positive cultures from chronic histoplasmosis could be expected to result from pretreatment of clinical specimens in glycine buffer pH 10.0 prior to culture.
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spelling pubmed-71019752020-03-31 Studies on histoplasmosis IV. Elution characteristics of the Histo-inhibitory factor (HIF) O'Hern, E. M. Fishbein, L. Mycopathol Mycol Appl Article The histo-inhibitory factor (HIF) derived from homogenates of liver or kidney from hamsters infected withHistoplasma capsulatum has been fractionated by column chromatography. It shows maximum absorption at 280 mµ, has a molecular weight of 142,000 and can be eluted from DEAE-cellulose or DEAE-Sephadex A—50 with 0.02 M phosphate — 4 M sodium chloride (1 : 1) HIF can be eluted fromHistoplasma yeast cells at pH 10.0, thus a greater number of positive cultures from chronic histoplasmosis could be expected to result from pretreatment of clinical specimens in glycine buffer pH 10.0 prior to culture. Kluwer Academic Publishers 1968 /pmc/articles/PMC7101975/ /pubmed/5748624 http://dx.doi.org/10.1007/BF02057164 Text en © Kluwer Academic Publishers 1968 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
O'Hern, E. M.
Fishbein, L.
Studies on histoplasmosis IV. Elution characteristics of the Histo-inhibitory factor (HIF)
title Studies on histoplasmosis IV. Elution characteristics of the Histo-inhibitory factor (HIF)
title_full Studies on histoplasmosis IV. Elution characteristics of the Histo-inhibitory factor (HIF)
title_fullStr Studies on histoplasmosis IV. Elution characteristics of the Histo-inhibitory factor (HIF)
title_full_unstemmed Studies on histoplasmosis IV. Elution characteristics of the Histo-inhibitory factor (HIF)
title_short Studies on histoplasmosis IV. Elution characteristics of the Histo-inhibitory factor (HIF)
title_sort studies on histoplasmosis iv. elution characteristics of the histo-inhibitory factor (hif)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101975/
https://www.ncbi.nlm.nih.gov/pubmed/5748624
http://dx.doi.org/10.1007/BF02057164
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