Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study

Estimation of the spread and advancement of Plasmodium falciparum artemisinin-resistant parasites can be done by probing polymorphisms in the kelch (Pfk13) domain (a validated molecular marker). This study aimed to provide baseline information for future artemisinin surveillance by analyzing the k13...

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Autores principales: Rana, Ramakanta, Ranjit, Manoranjan, Bal, Madhusmita, Khuntia, Hemant Kumar, Pati, Sanghamitra, Krishna, Sri, Das, Aparup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102476/
https://www.ncbi.nlm.nih.gov/pubmed/32258150
http://dx.doi.org/10.1155/2020/8475246
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author Rana, Ramakanta
Ranjit, Manoranjan
Bal, Madhusmita
Khuntia, Hemant Kumar
Pati, Sanghamitra
Krishna, Sri
Das, Aparup
author_facet Rana, Ramakanta
Ranjit, Manoranjan
Bal, Madhusmita
Khuntia, Hemant Kumar
Pati, Sanghamitra
Krishna, Sri
Das, Aparup
author_sort Rana, Ramakanta
collection PubMed
description Estimation of the spread and advancement of Plasmodium falciparum artemisinin-resistant parasites can be done by probing polymorphisms in the kelch (Pfk13) domain (a validated molecular marker). This study aimed to provide baseline information for future artemisinin surveillance by analyzing the k13-propeller domain in P. falciparum field isolates collected from 24 study areas in 14 malaria hot spots of Odisha (previously Orissa) during July 2018-January 2019. A total of 178 P. falciparum mono infections were assessed. An 849-base pair fragment encoding the Pfk13 propeller was amplified by nested polymerase chain reaction and sequenced in both directions (PCR). After DNA alignment with the 3D7 reference sequence, all samples were found to be wild type. It can be anticipated that malaria public health is not under direct threat in Odisha relating to ART resistance.
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spelling pubmed-71024762020-04-04 Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study Rana, Ramakanta Ranjit, Manoranjan Bal, Madhusmita Khuntia, Hemant Kumar Pati, Sanghamitra Krishna, Sri Das, Aparup Biomed Res Int Research Article Estimation of the spread and advancement of Plasmodium falciparum artemisinin-resistant parasites can be done by probing polymorphisms in the kelch (Pfk13) domain (a validated molecular marker). This study aimed to provide baseline information for future artemisinin surveillance by analyzing the k13-propeller domain in P. falciparum field isolates collected from 24 study areas in 14 malaria hot spots of Odisha (previously Orissa) during July 2018-January 2019. A total of 178 P. falciparum mono infections were assessed. An 849-base pair fragment encoding the Pfk13 propeller was amplified by nested polymerase chain reaction and sequenced in both directions (PCR). After DNA alignment with the 3D7 reference sequence, all samples were found to be wild type. It can be anticipated that malaria public health is not under direct threat in Odisha relating to ART resistance. Hindawi 2020-03-15 /pmc/articles/PMC7102476/ /pubmed/32258150 http://dx.doi.org/10.1155/2020/8475246 Text en Copyright © 2020 Ramakanta Rana et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rana, Ramakanta
Ranjit, Manoranjan
Bal, Madhusmita
Khuntia, Hemant Kumar
Pati, Sanghamitra
Krishna, Sri
Das, Aparup
Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study
title Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study
title_full Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study
title_fullStr Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study
title_full_unstemmed Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study
title_short Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study
title_sort sequence analysis of the k13-propeller gene in artemisinin challenging plasmodium falciparum isolates from malaria endemic areas of odisha, india: a molecular surveillance study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102476/
https://www.ncbi.nlm.nih.gov/pubmed/32258150
http://dx.doi.org/10.1155/2020/8475246
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