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Recent advances and perspectives of nucleic acid detection for coronavirus

The recent pneumonia outbreak caused by a novel coronavirus (SARS-CoV-2) is posing a great threat to global public health. Therefore, rapid and accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments, saving people’s lives and preventing epidemics. It is...

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Detalles Bibliográficos
Autores principales: Shen, Minzhe, Zhou, Ying, Ye, Jiawei, Abdullah AL-maskri, Abdu Ahmed, Kang, Yu, Zeng, Su, Cai, Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Xi'an Jiaotong University 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102540/
https://www.ncbi.nlm.nih.gov/pubmed/32292623
http://dx.doi.org/10.1016/j.jpha.2020.02.010
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author Shen, Minzhe
Zhou, Ying
Ye, Jiawei
Abdullah AL-maskri, Abdu Ahmed
Kang, Yu
Zeng, Su
Cai, Sheng
author_facet Shen, Minzhe
Zhou, Ying
Ye, Jiawei
Abdullah AL-maskri, Abdu Ahmed
Kang, Yu
Zeng, Su
Cai, Sheng
author_sort Shen, Minzhe
collection PubMed
description The recent pneumonia outbreak caused by a novel coronavirus (SARS-CoV-2) is posing a great threat to global public health. Therefore, rapid and accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments, saving people’s lives and preventing epidemics. It is important to establish a quick standard diagnostic test for the detection of the infectious disease (COVID-19) to prevent subsequent secondary spread. Polymerase chain reaction (PCR) is regarded as a gold standard test for the molecular diagnosis of viral and bacterial infections with high sensitivity and specificity. Isothermal nucleic acid amplification is considered to be a highly promising candidate method due to its fundamental advantage in quick procedure time at constant temperature without thermocycler operation. A variety of improved or new approaches also have been developed. This review summarizes the currently available detection methods for coronavirus nucleic acid. It is anticipated that this will assist researchers and clinicians in developing better techniques for timely and effective detection of coronavirus infection.
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spelling pubmed-71025402020-03-31 Recent advances and perspectives of nucleic acid detection for coronavirus Shen, Minzhe Zhou, Ying Ye, Jiawei Abdullah AL-maskri, Abdu Ahmed Kang, Yu Zeng, Su Cai, Sheng J Pharm Anal Review Paper The recent pneumonia outbreak caused by a novel coronavirus (SARS-CoV-2) is posing a great threat to global public health. Therefore, rapid and accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments, saving people’s lives and preventing epidemics. It is important to establish a quick standard diagnostic test for the detection of the infectious disease (COVID-19) to prevent subsequent secondary spread. Polymerase chain reaction (PCR) is regarded as a gold standard test for the molecular diagnosis of viral and bacterial infections with high sensitivity and specificity. Isothermal nucleic acid amplification is considered to be a highly promising candidate method due to its fundamental advantage in quick procedure time at constant temperature without thermocycler operation. A variety of improved or new approaches also have been developed. This review summarizes the currently available detection methods for coronavirus nucleic acid. It is anticipated that this will assist researchers and clinicians in developing better techniques for timely and effective detection of coronavirus infection. Xi'an Jiaotong University 2020-04 2020-03-01 /pmc/articles/PMC7102540/ /pubmed/32292623 http://dx.doi.org/10.1016/j.jpha.2020.02.010 Text en © 2020 Xi'an Jiaotong University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Review Paper
Shen, Minzhe
Zhou, Ying
Ye, Jiawei
Abdullah AL-maskri, Abdu Ahmed
Kang, Yu
Zeng, Su
Cai, Sheng
Recent advances and perspectives of nucleic acid detection for coronavirus
title Recent advances and perspectives of nucleic acid detection for coronavirus
title_full Recent advances and perspectives of nucleic acid detection for coronavirus
title_fullStr Recent advances and perspectives of nucleic acid detection for coronavirus
title_full_unstemmed Recent advances and perspectives of nucleic acid detection for coronavirus
title_short Recent advances and perspectives of nucleic acid detection for coronavirus
title_sort recent advances and perspectives of nucleic acid detection for coronavirus
topic Review Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102540/
https://www.ncbi.nlm.nih.gov/pubmed/32292623
http://dx.doi.org/10.1016/j.jpha.2020.02.010
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