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Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2)
PURPOSE: Lianhuaqingwen (LH) as traditional Chinese medicine (TCM) formula has been used to treat influenza and exerted broad-spectrum antiviral effects on a series of influenza viruses and immune regulatory effects Ding et al. (2017). The goal of this study is to demonstrate the antiviral activity...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier Ltd.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102548/ https://www.ncbi.nlm.nih.gov/pubmed/32205232 http://dx.doi.org/10.1016/j.phrs.2020.104761 |
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author | Runfeng, Li Yunlong, Hou Jicheng, Huang Weiqi, Pan Qinhai, Ma Yongxia, Shi Chufang, Li Jin, Zhao Zhenhua, Jia Haiming, Jiang Kui, Zheng Shuxiang, Huang Jun, Dai Xiaobo, Li Xiaotao, Hou Lin, Wang Nanshan, Zhong Zifeng, Yang |
author_facet | Runfeng, Li Yunlong, Hou Jicheng, Huang Weiqi, Pan Qinhai, Ma Yongxia, Shi Chufang, Li Jin, Zhao Zhenhua, Jia Haiming, Jiang Kui, Zheng Shuxiang, Huang Jun, Dai Xiaobo, Li Xiaotao, Hou Lin, Wang Nanshan, Zhong Zifeng, Yang |
author_sort | Runfeng, Li |
collection | PubMed |
description | PURPOSE: Lianhuaqingwen (LH) as traditional Chinese medicine (TCM) formula has been used to treat influenza and exerted broad-spectrum antiviral effects on a series of influenza viruses and immune regulatory effects Ding et al. (2017). The goal of this study is to demonstrate the antiviral activity of LH against the novel SARS-CoV-2 virus and its potential effect in regulating host immune response. METHODS: The antiviral activity of LH against SARS-CoV-2 was assessed in Vero E6 cells using CPE and plaque reduction assay. The effect of LH on virion morphology was visualized under transmission electron microscope. Pro-inflammatory cytokine expression levels upon SARS-CoV-2 infection in Huh-7 cells were measured by real-time quantitative PCR assays. RESULTS: LH significantly inhibited SARS-CoV-2 replication in Vero E6 cells and markedly reduced pro-inflammatory cytokines (TNF-α, IL-6, CCL-2/MCP-1 and CXCL-10/IP-10) production at the mRNA levels. Furthermore, LH treatment resulted in abnormal particle morphology of virion in cells. CONCLUSIONS: LH significantly inhibits the SARS-COV-2 replication, affects virus morphology and exerts anti-inflammatory activity in vitro. These findings indicate that LH protects against the virus attack, making its use a novel strategy for controlling the COVID-19 disease. |
format | Online Article Text |
id | pubmed-7102548 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Published by Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71025482020-03-31 Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2) Runfeng, Li Yunlong, Hou Jicheng, Huang Weiqi, Pan Qinhai, Ma Yongxia, Shi Chufang, Li Jin, Zhao Zhenhua, Jia Haiming, Jiang Kui, Zheng Shuxiang, Huang Jun, Dai Xiaobo, Li Xiaotao, Hou Lin, Wang Nanshan, Zhong Zifeng, Yang Pharmacol Res Article PURPOSE: Lianhuaqingwen (LH) as traditional Chinese medicine (TCM) formula has been used to treat influenza and exerted broad-spectrum antiviral effects on a series of influenza viruses and immune regulatory effects Ding et al. (2017). The goal of this study is to demonstrate the antiviral activity of LH against the novel SARS-CoV-2 virus and its potential effect in regulating host immune response. METHODS: The antiviral activity of LH against SARS-CoV-2 was assessed in Vero E6 cells using CPE and plaque reduction assay. The effect of LH on virion morphology was visualized under transmission electron microscope. Pro-inflammatory cytokine expression levels upon SARS-CoV-2 infection in Huh-7 cells were measured by real-time quantitative PCR assays. RESULTS: LH significantly inhibited SARS-CoV-2 replication in Vero E6 cells and markedly reduced pro-inflammatory cytokines (TNF-α, IL-6, CCL-2/MCP-1 and CXCL-10/IP-10) production at the mRNA levels. Furthermore, LH treatment resulted in abnormal particle morphology of virion in cells. CONCLUSIONS: LH significantly inhibits the SARS-COV-2 replication, affects virus morphology and exerts anti-inflammatory activity in vitro. These findings indicate that LH protects against the virus attack, making its use a novel strategy for controlling the COVID-19 disease. Published by Elsevier Ltd. 2020-06 2020-03-20 /pmc/articles/PMC7102548/ /pubmed/32205232 http://dx.doi.org/10.1016/j.phrs.2020.104761 Text en © 2020 Published by Elsevier Ltd. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Runfeng, Li Yunlong, Hou Jicheng, Huang Weiqi, Pan Qinhai, Ma Yongxia, Shi Chufang, Li Jin, Zhao Zhenhua, Jia Haiming, Jiang Kui, Zheng Shuxiang, Huang Jun, Dai Xiaobo, Li Xiaotao, Hou Lin, Wang Nanshan, Zhong Zifeng, Yang Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2) |
title | Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2) |
title_full | Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2) |
title_fullStr | Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2) |
title_full_unstemmed | Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2) |
title_short | Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2) |
title_sort | lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (sars-cov-2) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102548/ https://www.ncbi.nlm.nih.gov/pubmed/32205232 http://dx.doi.org/10.1016/j.phrs.2020.104761 |
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