Measuring mRNA translation in neuronal processes and somata by tRNA-FRET

In neurons, the specific spatial and temporal localization of protein synthesis is of great importance for function and survival. Here, we visualized tRNA and protein synthesis events in fixed and live mouse primary cortical culture using fluorescently-labeled tRNAs. We were able to characterize the...

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Detalles Bibliográficos
Autores principales: Koltun, Bella, Ironi, Sivan, Gershoni-Emek, Noga, Barrera, Iliana, Hleihil, Mohammad, Nanguneri, Siddharth, Sasmal, Ranjan, Agasti, Sarit S, Nair, Deepak, Rosenblum, Kobi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102941/
https://www.ncbi.nlm.nih.gov/pubmed/31974573
http://dx.doi.org/10.1093/nar/gkaa042

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102941/
https://www.ncbi.nlm.nih.gov/pubmed/31974573
http://dx.doi.org/10.1093/nar/gkaa042

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