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Good guide, bad guide: spacer sequence-dependent cleavage efficiency of Cas12a

Genome editing has recently made a revolutionary development with the introduction of the CRISPR–Cas technology. The programmable CRISPR-associated Cas9 and Cas12a nucleases generate specific dsDNA breaks in the genome, after which host DNA-repair mechanisms can be manipulated to implement the desir...

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Detalles Bibliográficos
Autores principales:	Creutzburg, Sjoerd C A, Wu, Wen Y, Mohanraju, Prarthana, Swartjes, Thomas, Alkan, Ferhat, Gorodkin, Jan, Staals, Raymond H J, van der Oost, John
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2020
Materias:	RNA and RNA-protein complexes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102956/ https://www.ncbi.nlm.nih.gov/pubmed/31989168 http://dx.doi.org/10.1093/nar/gkz1240

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