Cargando…

Dynamic structural insights into the molecular mechanism of DNA unwinding by the bacteriophage T7 helicase

The hexametric T7 helicase (gp4) adopts a spiral lock-washer form and encircles a coil-like DNA (tracking) strand with two nucleotides bound to each subunit. However, the chemo-mechanical coupling mechanism in unwinding has yet to be elucidated. Here, we utilized nanotensioner-enhanced Förster reson...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ma, Jian-Bing, Chen, Ze, Xu, Chun-Hua, Huang, Xing-Yuan, Jia, Qi, Zou, Zhen-Yu, Mi, Chen-Yang, Ma, Dong-Fei, Lu, Ying, Zhang, Hui-Dong, Li, Ming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2020
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7102974/ https://www.ncbi.nlm.nih.gov/pubmed/32009150 http://dx.doi.org/10.1093/nar/gkaa057

Ejemplares similares

Unwinding forward and sliding back: an intermittent unwinding mode of the BLM helicase
por: Wang, Shuang, et al.
Publicado: (2015)

Evidence for a functional dimeric form of the PcrA helicase in DNA unwinding
por: Yang, Ye, et al.
Publicado: (2008)

Insights into the mechanism of a G-quadruplex-unwinding DEAH-box helicase
por: Chen, Michael C., et al.
Publicado: (2015)

Visualizing helicases unwinding DNA at the single molecule level
por: Fili, Natali, et al.
Publicado: (2010)

Regulation of Rep helicase unwinding by an auto-inhibitory subdomain
por: Makurath, Monika A, et al.
Publicado: (2019)

Repetitive RNA unwinding by RNA helicase A facilitates RNA annealing
por: Koh, Hye Ran, et al.
Publicado: (2014)

Cooperative translocation enhances the unwinding of duplex DNA by SARS coronavirus helicase nsP13
por: Lee, Na-Ra, et al.
Publicado: (2010)

Phosphate release contributes to the rate-limiting step for unwinding by an RNA helicase
por: Wang, Qixin, et al.
Publicado: (2010)

Identification of RNA helicases with unwinding activity on angiogenin-processed tRNAs
por: Drino, Aleksej, et al.
Publicado: (2023)

A conformational change in the helicase core is necessary but not sufficient for RNA unwinding by the DEAD box helicase YxiN
por: Karow, Anne R., et al.
Publicado: (2009)

Rapid unwinding of triplet repeat hairpins by Srs2 helicase of Saccharomyces cerevisiae
por: Dhar, Alok, et al.
Publicado: (2008)

Ring-shaped replicative helicase encircles double-stranded DNA during unwinding
por: Joo, Sihwa, et al.
Publicado: (2019)

Switching from single-stranded to double-stranded DNA limits the unwinding processivity of ring-shaped T7 DNA helicase
por: Jeong, Yong-Joo, et al.
Publicado: (2013)

The yeast Pif1p DNA helicase preferentially unwinds RNA–DNA substrates
por: Boulé, Jean-Baptiste, et al.
Publicado: (2007)

Human Pif1 helicase unwinds synthetic DNA structures resembling stalled DNA replication forks
por: George, Tresa, et al.
Publicado: (2009)

The helicase XPD unwinds bubble structures and is not stalled by DNA lesions removed by the nucleotide excision repair pathway
por: Rudolf, Jana, et al.
Publicado: (2010)

Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD
por: Chisty, Liisa T., et al.
Publicado: (2013)

Simultaneous binding to the tracking strand, displaced strand and the duplex of a DNA fork enhances unwinding by Dda helicase
por: Aarattuthodiyil, Suja, et al.
Publicado: (2014)

Characterization of an unusual bipolar helicase encoded by bacteriophage T5
por: Wong, Io Nam, et al.
Publicado: (2013)

The AddAB helicase–nuclease catalyses rapid and processive DNA unwinding using a single Superfamily 1A motor domain
por: Yeeles, Joseph T.P., et al.
Publicado: (2011)

The Pif1 signature motif of Pfh1 is necessary for both protein displacement and helicase unwinding activities, but is dispensable for strand-annealing activity
por: Mohammad, Jani B, et al.
Publicado: (2018)

The eukaryotic initiation factor eIF4H facilitates loop-binding, repetitive RNA unwinding by the eIF4A DEAD-box helicase
por: Sun, Yingjie, et al.
Publicado: (2012)

Pif1 is a force-regulated helicase
por: Li, Jing-Hua, et al.
Publicado: (2016)

Mycobacterium smegmatis RqlH defines a novel clade of bacterial RecQ-like DNA helicases with ATP-dependent 3′–5′ translocase and duplex unwinding activities
por: Ordonez, Heather, et al.
Publicado: (2012)

Human DDX21 binds and unwinds RNA guanine quadruplexes
por: McRae, Ewan K.S., et al.
Publicado: (2017)

Dynamics of DNA nicking and unwinding by the RepC–PcrA complex
por: Carrasco, Carolina, et al.
Publicado: (2020)

Werner syndrome protein works as a dimer for unwinding and replication fork regression
por: Shin, Soochul, et al.
Publicado: (2022)

Insights into the product release mechanism of dengue virus NS3 helicase
por: Adler, Natalia S, et al.
Publicado: (2022)

G4 Resolvase 1 tightly binds and unwinds unimolecular G4-DNA
por: Giri, Banabihari, et al.
Publicado: (2011)

The Mtr4 ratchet helix and arch domain both function to promote RNA unwinding
por: Taylor, Lacy L., et al.
Publicado: (2014)

Insights into the activation of the helicase Prp43 by biochemical studies and structural mass spectrometry
por: Christian, Henning, et al.
Publicado: (2014)

Unwinding of primer-templates by archaeal family-B DNA polymerases in response to template-strand uracil
por: Richardson, Tomas T., et al.
Publicado: (2013)

A DNA unwinding equilibrium serves as a checkpoint for CRISPR-Cas12a target discrimination
por: Singh, Jaideep, et al.
Publicado: (2023)

Active and passive mechanisms of helicases
por: Manosas, Maria, et al.
Publicado: (2010)

Structure–function analysis of the RNA polymerase cleft loops elucidates initial transcription, DNA unwinding and RNA displacement
por: Naji, Souad, et al.
Publicado: (2008)

Biochemical characterization of the helicase Sen1 provides new insights into the mechanisms of non-coding transcription termination
por: Han, Zhong, et al.
Publicado: (2017)

Single-stranded regions modulate conformational dynamics and ATPase activity of eIF4A to optimize 5′-UTR unwinding
por: Andreou, Alexandra Zoi, et al.
Publicado: (2019)

The putative RNase P motif in the DEAD box helicase Hera is dispensable for efficient interaction with RNA and helicase activity
por: Linden, Martin H., et al.
Publicado: (2008)

G-quadruplex and G-rich sequence stimulate Pif1p-catalyzed downstream duplex DNA unwinding through reducing waiting time at ss/dsDNA junction
por: Zhang, Bo, et al.
Publicado: (2016)

Structure-function analysis of the RNA helicase maleless
por: Izzo, Annalisa, et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_uu9jbcl94sk8o1k5tmmp11ldu4