Isoflurane Postconditioning Upregulates Phosphorylated Connexin 43 in the Middle Cerebral Artery Occlusion Model and Is Probably Associated with the TGF-β1/Smad2/3 Signaling Pathway

AIM: Connexin 43 (Cx43) has been identified to be important for cerebral ischemia/reperfusion (I/R) injury as well as protection from it. This study was aimed at investigating the relationship between phosphorylated Cx43 (p-Cx43), transforming growth factor-β1 (TGF-β1 (TGF- METHODS: The middle cereb...

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Autores principales: Yin, Jiangwen, Liu, Xuejiao, Wang, Ruixue, Ge, Mingyue, Xie, Liping, Zhai, Jingwen, Dai, Zhigang, Li, Yan, Wang, Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103038/
https://www.ncbi.nlm.nih.gov/pubmed/32258113
http://dx.doi.org/10.1155/2020/3451215
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author Yin, Jiangwen
Liu, Xuejiao
Wang, Ruixue
Ge, Mingyue
Xie, Liping
Zhai, Jingwen
Dai, Zhigang
Li, Yan
Wang, Sheng
author_facet Yin, Jiangwen
Liu, Xuejiao
Wang, Ruixue
Ge, Mingyue
Xie, Liping
Zhai, Jingwen
Dai, Zhigang
Li, Yan
Wang, Sheng
author_sort Yin, Jiangwen
collection PubMed
description AIM: Connexin 43 (Cx43) has been identified to be important for cerebral ischemia/reperfusion (I/R) injury as well as protection from it. This study was aimed at investigating the relationship between phosphorylated Cx43 (p-Cx43), transforming growth factor-β1 (TGF-β1 (TGF- METHODS: The middle cerebral artery occlusion (MCAO) model was induced in 96 male Sprague-Dawley rats, weighing 250-300 g. The rats were randomized into 12 groups, namely, sham, middle cerebral artery occlusion (MCAO)/I/R, I/R+1.5% ISPOC, I/R+LY2157299 (blocker of TGF-β1 (TGF-β1 (TGF-β1 (TGF-β1 (TGF- RESULTS: Neurological deficit scores, brain infarct volume, and damaged neurons in the I/R group significantly increased compared to those in the sham group (P < 0.05). However, in the ISPOC group, damage of the brain was significantly ameliorated (P < 0.05). However, in the ISPOC group, damage of the brain was significantly ameliorated (P < 0.05). However, in the ISPOC group, damage of the brain was significantly ameliorated (β1 (TGF-P < 0.05). However, in the ISPOC group, damage of the brain was significantly ameliorated (β1 (TGF-P < 0.05). However, in the ISPOC group, damage of the brain was significantly ameliorated (β1 (TGF-β1 (TGF-P < 0.05). However, in the ISPOC group, damage of the brain was significantly ameliorated ( CONCLUSION: Isoflurane postconditioning (ISPOC) may alleviate cerebral I/R injury through upregulating the expression of p-Cx43, and the TGF-β1/Smad2/3 signaling pathway may be involved in the process.β1 (TGF-
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spelling pubmed-71030382020-04-04 Isoflurane Postconditioning Upregulates Phosphorylated Connexin 43 in the Middle Cerebral Artery Occlusion Model and Is Probably Associated with the TGF-β1/Smad2/3 Signaling Pathway Yin, Jiangwen Liu, Xuejiao Wang, Ruixue Ge, Mingyue Xie, Liping Zhai, Jingwen Dai, Zhigang Li, Yan Wang, Sheng Biomed Res Int Research Article AIM: Connexin 43 (Cx43) has been identified to be important for cerebral ischemia/reperfusion (I/R) injury as well as protection from it. This study was aimed at investigating the relationship between phosphorylated Cx43 (p-Cx43), transforming growth factor-β1 (TGF-β1 (TGF- METHODS: The middle cerebral artery occlusion (MCAO) model was induced in 96 male Sprague-Dawley rats, weighing 250-300 g. The rats were randomized into 12 groups, namely, sham, middle cerebral artery occlusion (MCAO)/I/R, I/R+1.5% ISPOC, I/R+LY2157299 (blocker of TGF-β1 (TGF-β1 (TGF-β1 (TGF-β1 (TGF- RESULTS: Neurological deficit scores, brain infarct volume, and damaged neurons in the I/R group significantly increased compared to those in the sham group (P < 0.05). However, in the ISPOC group, damage of the brain was significantly ameliorated (P < 0.05). However, in the ISPOC group, damage of the brain was significantly ameliorated (P < 0.05). However, in the ISPOC group, damage of the brain was significantly ameliorated (β1 (TGF-P < 0.05). However, in the ISPOC group, damage of the brain was significantly ameliorated (β1 (TGF-P < 0.05). However, in the ISPOC group, damage of the brain was significantly ameliorated (β1 (TGF-β1 (TGF-P < 0.05). However, in the ISPOC group, damage of the brain was significantly ameliorated ( CONCLUSION: Isoflurane postconditioning (ISPOC) may alleviate cerebral I/R injury through upregulating the expression of p-Cx43, and the TGF-β1/Smad2/3 signaling pathway may be involved in the process.β1 (TGF- Hindawi 2020-03-17 /pmc/articles/PMC7103038/ /pubmed/32258113 http://dx.doi.org/10.1155/2020/3451215 Text en Copyright © 2020 Jiangwen Yin et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yin, Jiangwen
Liu, Xuejiao
Wang, Ruixue
Ge, Mingyue
Xie, Liping
Zhai, Jingwen
Dai, Zhigang
Li, Yan
Wang, Sheng
Isoflurane Postconditioning Upregulates Phosphorylated Connexin 43 in the Middle Cerebral Artery Occlusion Model and Is Probably Associated with the TGF-β1/Smad2/3 Signaling Pathway
title Isoflurane Postconditioning Upregulates Phosphorylated Connexin 43 in the Middle Cerebral Artery Occlusion Model and Is Probably Associated with the TGF-β1/Smad2/3 Signaling Pathway
title_full Isoflurane Postconditioning Upregulates Phosphorylated Connexin 43 in the Middle Cerebral Artery Occlusion Model and Is Probably Associated with the TGF-β1/Smad2/3 Signaling Pathway
title_fullStr Isoflurane Postconditioning Upregulates Phosphorylated Connexin 43 in the Middle Cerebral Artery Occlusion Model and Is Probably Associated with the TGF-β1/Smad2/3 Signaling Pathway
title_full_unstemmed Isoflurane Postconditioning Upregulates Phosphorylated Connexin 43 in the Middle Cerebral Artery Occlusion Model and Is Probably Associated with the TGF-β1/Smad2/3 Signaling Pathway
title_short Isoflurane Postconditioning Upregulates Phosphorylated Connexin 43 in the Middle Cerebral Artery Occlusion Model and Is Probably Associated with the TGF-β1/Smad2/3 Signaling Pathway
title_sort isoflurane postconditioning upregulates phosphorylated connexin 43 in the middle cerebral artery occlusion model and is probably associated with the tgf-β1/smad2/3 signaling pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103038/
https://www.ncbi.nlm.nih.gov/pubmed/32258113
http://dx.doi.org/10.1155/2020/3451215
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