Cargando…

Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma

PURPOSE: Human papillomavirus (HPV) antigens had been found in colorectal cancer (CRC) tissue, but little evidence demonstrates the association of HPV with oncogene mutations in CRC. We aim to elucidate the mutated genes that link HPV infection and CRC carcinogenesis. METHODS: Cancerous and adjacent...

Descripción completa

Detalles Bibliográficos
Autores principales: Qiu, Qiancheng, Li, Yazhen, Fan, Zhiqiang, Yao, Fen, Shen, Wenjun, Sun, Jiayu, Yuan, Yumeng, Chen, Jinghong, Cai, Leshan, Xie, Yanxuan, Liu, Kaixi, Chen, Xiang, Jiao, Xiaoyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103040/
https://www.ncbi.nlm.nih.gov/pubmed/32258125
http://dx.doi.org/10.1155/2020/5201587
_version_ 1783511966038360064
author Qiu, Qiancheng
Li, Yazhen
Fan, Zhiqiang
Yao, Fen
Shen, Wenjun
Sun, Jiayu
Yuan, Yumeng
Chen, Jinghong
Cai, Leshan
Xie, Yanxuan
Liu, Kaixi
Chen, Xiang
Jiao, Xiaoyang
author_facet Qiu, Qiancheng
Li, Yazhen
Fan, Zhiqiang
Yao, Fen
Shen, Wenjun
Sun, Jiayu
Yuan, Yumeng
Chen, Jinghong
Cai, Leshan
Xie, Yanxuan
Liu, Kaixi
Chen, Xiang
Jiao, Xiaoyang
author_sort Qiu, Qiancheng
collection PubMed
description PURPOSE: Human papillomavirus (HPV) antigens had been found in colorectal cancer (CRC) tissue, but little evidence demonstrates the association of HPV with oncogene mutations in CRC. We aim to elucidate the mutated genes that link HPV infection and CRC carcinogenesis. METHODS: Cancerous and adjacent noncancerous tissues were obtained from CRC patients. HPV antigen was measured by using the immunohistochemical (IHC) technique. The differentially expressed genes (DEGs) in HPV-positive and HPV-negative tumor tissues were measured by using TaqMan Array Plates. The target genes were validated with the qPCR method. RESULTS: 15 (31.9%) cases of CRC patients were observed to be HPV positive, in which HPV antigen was expressed in most tumor tissues rather than in adjacent noncancerous tissues. With TaqMan Array Plates analyses, we found that 39 differentially expressed genes (DEGs) were upregulated, while 17 DEGs were downregulated in HPV-positive CRC tissues compared with HPV-negative tissues. Four DEGs (MMP-7, MYC, WNT-5A, and AXIN2) were upregulated in tumor vs. normal tissues, or adenoma vs. normal tissue in TCGA, which was overlapped with our data. In the confirmation test, MMP-7, MYC, WNT-5A, and AXIN2 were upregulated in cancerous tissue compared with adjacent noncancerous tissue. MYC, WNT-5A, and AXIN2 were shown to be upregulated in HPV-positive CRC tissues when compared to HPV-negative tissues. CONCLUSION: HPV-encoding genome may integrate into the tumor genomes that involved in multiple signaling pathways. Further genomic and proteomic investigation is necessary for obtaining a more comprehensive knowledge of signaling pathways associated with the CRC carcinogenesis.
format Online
Article
Text
id pubmed-7103040
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-71030402020-04-04 Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma Qiu, Qiancheng Li, Yazhen Fan, Zhiqiang Yao, Fen Shen, Wenjun Sun, Jiayu Yuan, Yumeng Chen, Jinghong Cai, Leshan Xie, Yanxuan Liu, Kaixi Chen, Xiang Jiao, Xiaoyang Biomed Res Int Research Article PURPOSE: Human papillomavirus (HPV) antigens had been found in colorectal cancer (CRC) tissue, but little evidence demonstrates the association of HPV with oncogene mutations in CRC. We aim to elucidate the mutated genes that link HPV infection and CRC carcinogenesis. METHODS: Cancerous and adjacent noncancerous tissues were obtained from CRC patients. HPV antigen was measured by using the immunohistochemical (IHC) technique. The differentially expressed genes (DEGs) in HPV-positive and HPV-negative tumor tissues were measured by using TaqMan Array Plates. The target genes were validated with the qPCR method. RESULTS: 15 (31.9%) cases of CRC patients were observed to be HPV positive, in which HPV antigen was expressed in most tumor tissues rather than in adjacent noncancerous tissues. With TaqMan Array Plates analyses, we found that 39 differentially expressed genes (DEGs) were upregulated, while 17 DEGs were downregulated in HPV-positive CRC tissues compared with HPV-negative tissues. Four DEGs (MMP-7, MYC, WNT-5A, and AXIN2) were upregulated in tumor vs. normal tissues, or adenoma vs. normal tissue in TCGA, which was overlapped with our data. In the confirmation test, MMP-7, MYC, WNT-5A, and AXIN2 were upregulated in cancerous tissue compared with adjacent noncancerous tissue. MYC, WNT-5A, and AXIN2 were shown to be upregulated in HPV-positive CRC tissues when compared to HPV-negative tissues. CONCLUSION: HPV-encoding genome may integrate into the tumor genomes that involved in multiple signaling pathways. Further genomic and proteomic investigation is necessary for obtaining a more comprehensive knowledge of signaling pathways associated with the CRC carcinogenesis. Hindawi 2020-03-17 /pmc/articles/PMC7103040/ /pubmed/32258125 http://dx.doi.org/10.1155/2020/5201587 Text en Copyright © 2020 Qiancheng Qiu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Qiu, Qiancheng
Li, Yazhen
Fan, Zhiqiang
Yao, Fen
Shen, Wenjun
Sun, Jiayu
Yuan, Yumeng
Chen, Jinghong
Cai, Leshan
Xie, Yanxuan
Liu, Kaixi
Chen, Xiang
Jiao, Xiaoyang
Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma
title Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma
title_full Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma
title_fullStr Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma
title_full_unstemmed Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma
title_short Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma
title_sort gene expression analysis of human papillomavirus-associated colorectal carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103040/
https://www.ncbi.nlm.nih.gov/pubmed/32258125
http://dx.doi.org/10.1155/2020/5201587
work_keys_str_mv AT qiuqiancheng geneexpressionanalysisofhumanpapillomavirusassociatedcolorectalcarcinoma
AT liyazhen geneexpressionanalysisofhumanpapillomavirusassociatedcolorectalcarcinoma
AT fanzhiqiang geneexpressionanalysisofhumanpapillomavirusassociatedcolorectalcarcinoma
AT yaofen geneexpressionanalysisofhumanpapillomavirusassociatedcolorectalcarcinoma
AT shenwenjun geneexpressionanalysisofhumanpapillomavirusassociatedcolorectalcarcinoma
AT sunjiayu geneexpressionanalysisofhumanpapillomavirusassociatedcolorectalcarcinoma
AT yuanyumeng geneexpressionanalysisofhumanpapillomavirusassociatedcolorectalcarcinoma
AT chenjinghong geneexpressionanalysisofhumanpapillomavirusassociatedcolorectalcarcinoma
AT caileshan geneexpressionanalysisofhumanpapillomavirusassociatedcolorectalcarcinoma
AT xieyanxuan geneexpressionanalysisofhumanpapillomavirusassociatedcolorectalcarcinoma
AT liukaixi geneexpressionanalysisofhumanpapillomavirusassociatedcolorectalcarcinoma
AT chenxiang geneexpressionanalysisofhumanpapillomavirusassociatedcolorectalcarcinoma
AT jiaoxiaoyang geneexpressionanalysisofhumanpapillomavirusassociatedcolorectalcarcinoma