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Electron Microscopy Analysis of Viral Morphogenesis

This chapter reviews the use of standard transmission electron microscopy (TEM) of plastic-embedded material, as well as protocols for the immunolabeling of cryosections, in the analysis of viral interactions with cells. It focuses particularly on the assembly of two types of enveloped viruses: (1)...

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Detalles Bibliográficos
Autores principales: Pelchen‐Matthews, Annegret, Marsh, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103132/
https://www.ncbi.nlm.nih.gov/pubmed/17327172
http://dx.doi.org/10.1016/S0091-679X(06)79020-3
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author Pelchen‐Matthews, Annegret
Marsh, Mark
author_facet Pelchen‐Matthews, Annegret
Marsh, Mark
author_sort Pelchen‐Matthews, Annegret
collection PubMed
description This chapter reviews the use of standard transmission electron microscopy (TEM) of plastic-embedded material, as well as protocols for the immunolabeling of cryosections, in the analysis of viral interactions with cells. It focuses particularly on the assembly of two types of enveloped viruses: (1) the beta herpesvirus—human cytomegalovirus (HCMV), and (2) the primate lentiviruses—the simian and human immunodeficiency viruses (SIV and HIV). The chapter discusses the ways EM is used to identify morphological features of the various stages in the assembly of virus particles, to distinguish immature and mature particles, or to analyze steps involved in the acquisition of lipid membranes by enveloped viruses. In addition, it demonstrates the way immunolabeling allows the quantification of viral components, even in individual virus particles, and comparisons between particles at different locations in the cell or at different stages in viral assembly. Together with the newly developed methods for electron tomography and correlative immunofluorescence studies and EM, huge potential exists to unravel more details about virus assembly in the near future.
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spelling pubmed-71031322020-03-31 Electron Microscopy Analysis of Viral Morphogenesis Pelchen‐Matthews, Annegret Marsh, Mark Methods Cell Biol Article This chapter reviews the use of standard transmission electron microscopy (TEM) of plastic-embedded material, as well as protocols for the immunolabeling of cryosections, in the analysis of viral interactions with cells. It focuses particularly on the assembly of two types of enveloped viruses: (1) the beta herpesvirus—human cytomegalovirus (HCMV), and (2) the primate lentiviruses—the simian and human immunodeficiency viruses (SIV and HIV). The chapter discusses the ways EM is used to identify morphological features of the various stages in the assembly of virus particles, to distinguish immature and mature particles, or to analyze steps involved in the acquisition of lipid membranes by enveloped viruses. In addition, it demonstrates the way immunolabeling allows the quantification of viral components, even in individual virus particles, and comparisons between particles at different locations in the cell or at different stages in viral assembly. Together with the newly developed methods for electron tomography and correlative immunofluorescence studies and EM, huge potential exists to unravel more details about virus assembly in the near future. Elsevier Inc. 2007 2007-02-25 /pmc/articles/PMC7103132/ /pubmed/17327172 http://dx.doi.org/10.1016/S0091-679X(06)79020-3 Text en Copyright © 2007 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Pelchen‐Matthews, Annegret
Marsh, Mark
Electron Microscopy Analysis of Viral Morphogenesis
title Electron Microscopy Analysis of Viral Morphogenesis
title_full Electron Microscopy Analysis of Viral Morphogenesis
title_fullStr Electron Microscopy Analysis of Viral Morphogenesis
title_full_unstemmed Electron Microscopy Analysis of Viral Morphogenesis
title_short Electron Microscopy Analysis of Viral Morphogenesis
title_sort electron microscopy analysis of viral morphogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103132/
https://www.ncbi.nlm.nih.gov/pubmed/17327172
http://dx.doi.org/10.1016/S0091-679X(06)79020-3
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