Heparan sulfate is a binding molecule but not a receptor for CEACAM1-independent infection of murine coronavirus

A highly neurovirulent mouse hepatitis virus (MHV) JHMV strain (wt) with receptor (MHVR)-independent infection activity and its low-virulent mutant srr7 without such activity were found to attach to MHVR-negative, non-permissive BHK cells. To identify the molecule that interacts with JHMV, we focuse...

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Detalles Bibliográficos
Autores principales: Watanabe, Rie, Sawicki, Stanley G., Taguchi, Fumihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2007
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103320/
https://www.ncbi.nlm.nih.gov/pubmed/17692355
http://dx.doi.org/10.1016/j.virol.2007.06.034
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author Watanabe, Rie
Sawicki, Stanley G.
Taguchi, Fumihiro
author_facet Watanabe, Rie
Sawicki, Stanley G.
Taguchi, Fumihiro
author_sort Watanabe, Rie
collection PubMed
description A highly neurovirulent mouse hepatitis virus (MHV) JHMV strain (wt) with receptor (MHVR)-independent infection activity and its low-virulent mutant srr7 without such activity were found to attach to MHVR-negative, non-permissive BHK cells. To identify the molecule that interacts with JHMV, we focused on heparan sulfate (HS) since it works as a receptor of a mutant MHV-rec1 that infects in an MHVR-independent fashion. The present study indicates that HS interacts with both wt JHMV and srr7 but it does not function as an entry receptor as it apparently does for MHV-rec1. Furthermore, HS failed to serve as an entry receptor in the MHVR-independent infection of wt JHMV, indicating that HS is not a host factor that wt JHMV utilizes in an MHVR-independent infection.
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spelling pubmed-71033202020-03-31 Heparan sulfate is a binding molecule but not a receptor for CEACAM1-independent infection of murine coronavirus Watanabe, Rie Sawicki, Stanley G. Taguchi, Fumihiro Virology Rapid Communication A highly neurovirulent mouse hepatitis virus (MHV) JHMV strain (wt) with receptor (MHVR)-independent infection activity and its low-virulent mutant srr7 without such activity were found to attach to MHVR-negative, non-permissive BHK cells. To identify the molecule that interacts with JHMV, we focused on heparan sulfate (HS) since it works as a receptor of a mutant MHV-rec1 that infects in an MHVR-independent fashion. The present study indicates that HS interacts with both wt JHMV and srr7 but it does not function as an entry receptor as it apparently does for MHV-rec1. Furthermore, HS failed to serve as an entry receptor in the MHVR-independent infection of wt JHMV, indicating that HS is not a host factor that wt JHMV utilizes in an MHVR-independent infection. Elsevier Inc. 2007-09-15 2007-08-09 /pmc/articles/PMC7103320/ /pubmed/17692355 http://dx.doi.org/10.1016/j.virol.2007.06.034 Text en Copyright © 2007 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Rapid Communication
Watanabe, Rie
Sawicki, Stanley G.
Taguchi, Fumihiro
Heparan sulfate is a binding molecule but not a receptor for CEACAM1-independent infection of murine coronavirus
title Heparan sulfate is a binding molecule but not a receptor for CEACAM1-independent infection of murine coronavirus
title_full Heparan sulfate is a binding molecule but not a receptor for CEACAM1-independent infection of murine coronavirus
title_fullStr Heparan sulfate is a binding molecule but not a receptor for CEACAM1-independent infection of murine coronavirus
title_full_unstemmed Heparan sulfate is a binding molecule but not a receptor for CEACAM1-independent infection of murine coronavirus
title_short Heparan sulfate is a binding molecule but not a receptor for CEACAM1-independent infection of murine coronavirus
title_sort heparan sulfate is a binding molecule but not a receptor for ceacam1-independent infection of murine coronavirus
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103320/
https://www.ncbi.nlm.nih.gov/pubmed/17692355
http://dx.doi.org/10.1016/j.virol.2007.06.034
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