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Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes

Infectious Bronchitis Virus, a member of the Coronaviridae, is a respiratory pathogen in poultry. We found that in vitro stimulation with IBV resulted in ChIFN-γ production in splenocytes of both infected birds and uninfected birds. The non-specific stimulation did not occur when other avian viruses...

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Autores principales: Ariaans, Mark P., van de Haar, Peter M., Hensen, Evert J., Vervelde, Lonneke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103388/
https://www.ncbi.nlm.nih.gov/pubmed/19070879
http://dx.doi.org/10.1016/j.virol.2008.11.021
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author Ariaans, Mark P.
van de Haar, Peter M.
Hensen, Evert J.
Vervelde, Lonneke
author_facet Ariaans, Mark P.
van de Haar, Peter M.
Hensen, Evert J.
Vervelde, Lonneke
author_sort Ariaans, Mark P.
collection PubMed
description Infectious Bronchitis Virus, a member of the Coronaviridae, is a respiratory pathogen in poultry. We found that in vitro stimulation with IBV resulted in ChIFN-γ production in splenocytes of both infected birds and uninfected birds. The non-specific stimulation did not occur when other avian viruses or other coronaviruses were used or when mammalian cells were stimulated with IBV. Inactivation of IBV reduced ChIFN-γ production, but ChIFN-γ remained elevated compared to unstimulated cells. An increase in ChIFN-γ mRNA was detected in splenocytes from IBV-infected and uninfected chickens as early as 1 h after stimulation with IBV. These results indicate that IBV acts as a polyclonal stimulus, inducing a rapid production of IFN-γ even without previous exposure to the virus.
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spelling pubmed-71033882020-03-31 Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes Ariaans, Mark P. van de Haar, Peter M. Hensen, Evert J. Vervelde, Lonneke Virology Article Infectious Bronchitis Virus, a member of the Coronaviridae, is a respiratory pathogen in poultry. We found that in vitro stimulation with IBV resulted in ChIFN-γ production in splenocytes of both infected birds and uninfected birds. The non-specific stimulation did not occur when other avian viruses or other coronaviruses were used or when mammalian cells were stimulated with IBV. Inactivation of IBV reduced ChIFN-γ production, but ChIFN-γ remained elevated compared to unstimulated cells. An increase in ChIFN-γ mRNA was detected in splenocytes from IBV-infected and uninfected chickens as early as 1 h after stimulation with IBV. These results indicate that IBV acts as a polyclonal stimulus, inducing a rapid production of IFN-γ even without previous exposure to the virus. Elsevier Inc. 2009-03-01 2008-12-13 /pmc/articles/PMC7103388/ /pubmed/19070879 http://dx.doi.org/10.1016/j.virol.2008.11.021 Text en Copyright © 2008 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Ariaans, Mark P.
van de Haar, Peter M.
Hensen, Evert J.
Vervelde, Lonneke
Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes
title Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes
title_full Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes
title_fullStr Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes
title_full_unstemmed Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes
title_short Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes
title_sort infectious bronchitis virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103388/
https://www.ncbi.nlm.nih.gov/pubmed/19070879
http://dx.doi.org/10.1016/j.virol.2008.11.021
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