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Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes
Infectious Bronchitis Virus, a member of the Coronaviridae, is a respiratory pathogen in poultry. We found that in vitro stimulation with IBV resulted in ChIFN-γ production in splenocytes of both infected birds and uninfected birds. The non-specific stimulation did not occur when other avian viruses...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103388/ https://www.ncbi.nlm.nih.gov/pubmed/19070879 http://dx.doi.org/10.1016/j.virol.2008.11.021 |
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author | Ariaans, Mark P. van de Haar, Peter M. Hensen, Evert J. Vervelde, Lonneke |
author_facet | Ariaans, Mark P. van de Haar, Peter M. Hensen, Evert J. Vervelde, Lonneke |
author_sort | Ariaans, Mark P. |
collection | PubMed |
description | Infectious Bronchitis Virus, a member of the Coronaviridae, is a respiratory pathogen in poultry. We found that in vitro stimulation with IBV resulted in ChIFN-γ production in splenocytes of both infected birds and uninfected birds. The non-specific stimulation did not occur when other avian viruses or other coronaviruses were used or when mammalian cells were stimulated with IBV. Inactivation of IBV reduced ChIFN-γ production, but ChIFN-γ remained elevated compared to unstimulated cells. An increase in ChIFN-γ mRNA was detected in splenocytes from IBV-infected and uninfected chickens as early as 1 h after stimulation with IBV. These results indicate that IBV acts as a polyclonal stimulus, inducing a rapid production of IFN-γ even without previous exposure to the virus. |
format | Online Article Text |
id | pubmed-7103388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71033882020-03-31 Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes Ariaans, Mark P. van de Haar, Peter M. Hensen, Evert J. Vervelde, Lonneke Virology Article Infectious Bronchitis Virus, a member of the Coronaviridae, is a respiratory pathogen in poultry. We found that in vitro stimulation with IBV resulted in ChIFN-γ production in splenocytes of both infected birds and uninfected birds. The non-specific stimulation did not occur when other avian viruses or other coronaviruses were used or when mammalian cells were stimulated with IBV. Inactivation of IBV reduced ChIFN-γ production, but ChIFN-γ remained elevated compared to unstimulated cells. An increase in ChIFN-γ mRNA was detected in splenocytes from IBV-infected and uninfected chickens as early as 1 h after stimulation with IBV. These results indicate that IBV acts as a polyclonal stimulus, inducing a rapid production of IFN-γ even without previous exposure to the virus. Elsevier Inc. 2009-03-01 2008-12-13 /pmc/articles/PMC7103388/ /pubmed/19070879 http://dx.doi.org/10.1016/j.virol.2008.11.021 Text en Copyright © 2008 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Ariaans, Mark P. van de Haar, Peter M. Hensen, Evert J. Vervelde, Lonneke Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes |
title | Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes |
title_full | Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes |
title_fullStr | Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes |
title_full_unstemmed | Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes |
title_short | Infectious Bronchitis Virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes |
title_sort | infectious bronchitis virus induces acute interferon-gamma production through polyclonal stimulation of chicken leukocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103388/ https://www.ncbi.nlm.nih.gov/pubmed/19070879 http://dx.doi.org/10.1016/j.virol.2008.11.021 |
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