cis-Preferential requirement of a − 1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1

The genome of Red clover necrotic mosaic virus (RCNMV) consists of RNA1 and RNA2. RNA1 encodes N-terminally overlapping replication proteins, p27 and p88, which are translated in a cap-independent manner. The 3′ untranslated region of RNA1 contains RNA elements essential for cap-independent translat...

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Autores principales: Okamoto, Kimiyuki, Nagano, Hideaki, Iwakawa, Hirooki, Mizumoto, Hiroyuki, Takeda, Atsushi, Kaido, Masanori, Mise, Kazuyuki, Okuno, Tetsuro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103404/
https://www.ncbi.nlm.nih.gov/pubmed/18308359
http://dx.doi.org/10.1016/j.virol.2008.02.004
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author Okamoto, Kimiyuki
Nagano, Hideaki
Iwakawa, Hirooki
Mizumoto, Hiroyuki
Takeda, Atsushi
Kaido, Masanori
Mise, Kazuyuki
Okuno, Tetsuro
author_facet Okamoto, Kimiyuki
Nagano, Hideaki
Iwakawa, Hirooki
Mizumoto, Hiroyuki
Takeda, Atsushi
Kaido, Masanori
Mise, Kazuyuki
Okuno, Tetsuro
author_sort Okamoto, Kimiyuki
collection PubMed
description The genome of Red clover necrotic mosaic virus (RCNMV) consists of RNA1 and RNA2. RNA1 encodes N-terminally overlapping replication proteins, p27 and p88, which are translated in a cap-independent manner. The 3′ untranslated region of RNA1 contains RNA elements essential for cap-independent translation and negative-strand RNA synthesis. In this study, we investigated how p27 and p88 were engaged in the replication of RCNMV genomic RNAs by using DNA vectors or in vitro transcribed RNAs in protoplasts and in a cell-free extract of evacuolated BY-2 protoplasts. Our results show a cis-preferential requirement of p88, but not of p27, for the replication of RNA1. This mechanism might help to facilitate a switch in the role of RNA1 from mRNA to a replication template.
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spelling pubmed-71034042020-03-31 cis-Preferential requirement of a − 1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1 Okamoto, Kimiyuki Nagano, Hideaki Iwakawa, Hirooki Mizumoto, Hiroyuki Takeda, Atsushi Kaido, Masanori Mise, Kazuyuki Okuno, Tetsuro Virology Article The genome of Red clover necrotic mosaic virus (RCNMV) consists of RNA1 and RNA2. RNA1 encodes N-terminally overlapping replication proteins, p27 and p88, which are translated in a cap-independent manner. The 3′ untranslated region of RNA1 contains RNA elements essential for cap-independent translation and negative-strand RNA synthesis. In this study, we investigated how p27 and p88 were engaged in the replication of RCNMV genomic RNAs by using DNA vectors or in vitro transcribed RNAs in protoplasts and in a cell-free extract of evacuolated BY-2 protoplasts. Our results show a cis-preferential requirement of p88, but not of p27, for the replication of RNA1. This mechanism might help to facilitate a switch in the role of RNA1 from mRNA to a replication template. Elsevier Inc. 2008-05-25 2008-03-04 /pmc/articles/PMC7103404/ /pubmed/18308359 http://dx.doi.org/10.1016/j.virol.2008.02.004 Text en Copyright © 2008 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Okamoto, Kimiyuki
Nagano, Hideaki
Iwakawa, Hirooki
Mizumoto, Hiroyuki
Takeda, Atsushi
Kaido, Masanori
Mise, Kazuyuki
Okuno, Tetsuro
cis-Preferential requirement of a − 1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1
title cis-Preferential requirement of a − 1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1
title_full cis-Preferential requirement of a − 1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1
title_fullStr cis-Preferential requirement of a − 1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1
title_full_unstemmed cis-Preferential requirement of a − 1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1
title_short cis-Preferential requirement of a − 1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1
title_sort cis-preferential requirement of a − 1 frameshift product p88 for the replication of red clover necrotic mosaic virus rna1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103404/
https://www.ncbi.nlm.nih.gov/pubmed/18308359
http://dx.doi.org/10.1016/j.virol.2008.02.004
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