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Optimized Generation of Primary Human Epithelial Cells from Larynx and Hypopharynx: A Site-Specific Epithelial Model for Reflux Research
Laryngopharyngeal reflux (LPR) induces a differential damage effect on several anatomic sites within the larynx and hypopharynx; therefore, an in vitro model is needed for each anatomic site. This study aimed to establish a primary culture method for human laryngeal and hypopharyngeal epithelial cel...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103601/ https://www.ncbi.nlm.nih.gov/pubmed/30917697 http://dx.doi.org/10.1177/0963689719838478 |
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author | Mo, Ting-Ting Tan, Jia-Jie Wang, Mei-Gui Dai, Yuan-Feng Liu, Xiong Li, Xiang-Ping |
author_facet | Mo, Ting-Ting Tan, Jia-Jie Wang, Mei-Gui Dai, Yuan-Feng Liu, Xiong Li, Xiang-Ping |
author_sort | Mo, Ting-Ting |
collection | PubMed |
description | Laryngopharyngeal reflux (LPR) induces a differential damage effect on several anatomic sites within the larynx and hypopharynx; therefore, an in vitro model is needed for each anatomic site. This study aimed to establish a primary culture method for human laryngeal and hypopharyngeal epithelial cells derived from multiple anatomic sites. Surgical mucosa specimens were treated with a two-step enzymatic strategy to establish a primary culture. Of the 46 samples, primary cultivation was achieved successfully with 36 samples, and the positive ratio was 78.3%. In addition, flow cytometry revealed that these primary cells were epithelial cells with a purity of 94.9%. The proliferative ability was confirmed by positive staining for Ki-67. Laryngeal and hypopharyngeal epithelial cells from multiple sites exhibited similar epithelial morphology and positive cytokeratin expression. These cells can be cultured to passage 4. In summary, we successfully established the in vitro epithelial model of larynx and hypopharynx subsites, which may potentially be used as a platform for reflux research, especially for site-specific damage effect. |
format | Online Article Text |
id | pubmed-7103601 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-71036012020-04-03 Optimized Generation of Primary Human Epithelial Cells from Larynx and Hypopharynx: A Site-Specific Epithelial Model for Reflux Research Mo, Ting-Ting Tan, Jia-Jie Wang, Mei-Gui Dai, Yuan-Feng Liu, Xiong Li, Xiang-Ping Cell Transplant Original Articles Laryngopharyngeal reflux (LPR) induces a differential damage effect on several anatomic sites within the larynx and hypopharynx; therefore, an in vitro model is needed for each anatomic site. This study aimed to establish a primary culture method for human laryngeal and hypopharyngeal epithelial cells derived from multiple anatomic sites. Surgical mucosa specimens were treated with a two-step enzymatic strategy to establish a primary culture. Of the 46 samples, primary cultivation was achieved successfully with 36 samples, and the positive ratio was 78.3%. In addition, flow cytometry revealed that these primary cells were epithelial cells with a purity of 94.9%. The proliferative ability was confirmed by positive staining for Ki-67. Laryngeal and hypopharyngeal epithelial cells from multiple sites exhibited similar epithelial morphology and positive cytokeratin expression. These cells can be cultured to passage 4. In summary, we successfully established the in vitro epithelial model of larynx and hypopharynx subsites, which may potentially be used as a platform for reflux research, especially for site-specific damage effect. SAGE Publications 2019-03-27 2019-05 /pmc/articles/PMC7103601/ /pubmed/30917697 http://dx.doi.org/10.1177/0963689719838478 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Articles Mo, Ting-Ting Tan, Jia-Jie Wang, Mei-Gui Dai, Yuan-Feng Liu, Xiong Li, Xiang-Ping Optimized Generation of Primary Human Epithelial Cells from Larynx and Hypopharynx: A Site-Specific Epithelial Model for Reflux Research |
title | Optimized Generation of Primary Human Epithelial Cells from Larynx and
Hypopharynx: A Site-Specific Epithelial Model for Reflux Research |
title_full | Optimized Generation of Primary Human Epithelial Cells from Larynx and
Hypopharynx: A Site-Specific Epithelial Model for Reflux Research |
title_fullStr | Optimized Generation of Primary Human Epithelial Cells from Larynx and
Hypopharynx: A Site-Specific Epithelial Model for Reflux Research |
title_full_unstemmed | Optimized Generation of Primary Human Epithelial Cells from Larynx and
Hypopharynx: A Site-Specific Epithelial Model for Reflux Research |
title_short | Optimized Generation of Primary Human Epithelial Cells from Larynx and
Hypopharynx: A Site-Specific Epithelial Model for Reflux Research |
title_sort | optimized generation of primary human epithelial cells from larynx and
hypopharynx: a site-specific epithelial model for reflux research |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103601/ https://www.ncbi.nlm.nih.gov/pubmed/30917697 http://dx.doi.org/10.1177/0963689719838478 |
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