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Real-Time Measurement of the Liquid Amount in Cryo-Electron Microscopy Grids Using Laser Diffraction of Regular 2-D Holes of the Grids

Cryo-electron microscopy (cryo-EM) is now the first choice to determine the high-resolution structures of huge protein complexes. Grids with two-dimensional arrays of holes covered with a carbon film are typically used in cryo-EM. Although semi-automatic plungers are available, notable trial-and-err...

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Detalles Bibliográficos
Autores principales: Ahn, Jinsook, Lee, Dukwon, Jo, Inseong, Jeong, Hyeongseop, Hyun, Jae-Kyung, Woo, Jae-Sung, Choi, Sang-Ho, Ha, Nam-Chul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103880/
https://www.ncbi.nlm.nih.gov/pubmed/32150795
http://dx.doi.org/10.14348/molcells.2020.2238