Cargando…
Seroprevalence of MERS-CoV in healthy adults in western Saudi Arabia, 2011–2016
BACKGROUND: The Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly recognized zoonotic coronavirus. Current evidence confirms the role of dromedaries in primary human infections but does not explain the sporadic community cases. However, asymptomatic or subclinical cases could repres...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier Ltd on behalf of King Saud Bin Abdulaziz University for Health Sciences.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7104088/ https://www.ncbi.nlm.nih.gov/pubmed/32005618 http://dx.doi.org/10.1016/j.jiph.2020.01.001 |
_version_ | 1783512172750438400 |
---|---|
author | Degnah, Afnan A. Al-amri, Sawsan S. Hassan, Ahmed M. Almasoud, Abdulrahman S. Mousa, Manar Almahboub, Sarah A. Alhabbab, Rowa Y. Mirza, Ahmed A. Hindawi, Salwa I. Alharbi, Naif Khalaf Azhar, Esam I. Hashem, Anwar M. |
author_facet | Degnah, Afnan A. Al-amri, Sawsan S. Hassan, Ahmed M. Almasoud, Abdulrahman S. Mousa, Manar Almahboub, Sarah A. Alhabbab, Rowa Y. Mirza, Ahmed A. Hindawi, Salwa I. Alharbi, Naif Khalaf Azhar, Esam I. Hashem, Anwar M. |
author_sort | Degnah, Afnan A. |
collection | PubMed |
description | BACKGROUND: The Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly recognized zoonotic coronavirus. Current evidence confirms the role of dromedaries in primary human infections but does not explain the sporadic community cases. However, asymptomatic or subclinical cases could represent a possible source of infection in the community. METHODS: Archived human sera (7461) collected between 2011 and 2016 from healthy adult blood donors from 50 different nationalities in the western part of Saudi Arabia were obtained for MERS-CoV seroprevalence investigation. Samples were tested for MERS-CoV S1-specific antibodies (Abs) by ELISA and confirmed by testing for neutralizing Abs (nAbs) using both pseudotyped and live virus neutralization assays. RESULTS: Out of 7461 samples, 174 sera from individuals with 18 different nationalities were ELISA positive (2.3%, 95% CI 2.0–2.7). Presence of nAbs was confirmed in 17 samples (0.23%, 95% CI 0.1–0.4) of which one sample exhibited positivity in both neutralization assays. Confirmed seropositivity was identified in young (15–44 years) men and women from Saudi Arabia, Egypt, Yemen, Pakistan, Palestine, Sudan, and India without significant preference. CONCLUSIONS: An increasing trend of MERS-CoV seroprevalence was observed in the general population in western Saudi Arabia, suggesting that asymptomatic or mild infections might exist and act as an unrecognized source of infection. Seropositivity of individuals from different nationalities underscores the potential MERS exportation outside of the Arabian Peninsula. Thus, enhanced and continuous surveillance is highly warranted. |
format | Online Article Text |
id | pubmed-7104088 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Published by Elsevier Ltd on behalf of King Saud Bin Abdulaziz University for Health Sciences. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71040882020-03-31 Seroprevalence of MERS-CoV in healthy adults in western Saudi Arabia, 2011–2016 Degnah, Afnan A. Al-amri, Sawsan S. Hassan, Ahmed M. Almasoud, Abdulrahman S. Mousa, Manar Almahboub, Sarah A. Alhabbab, Rowa Y. Mirza, Ahmed A. Hindawi, Salwa I. Alharbi, Naif Khalaf Azhar, Esam I. Hashem, Anwar M. J Infect Public Health Article BACKGROUND: The Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly recognized zoonotic coronavirus. Current evidence confirms the role of dromedaries in primary human infections but does not explain the sporadic community cases. However, asymptomatic or subclinical cases could represent a possible source of infection in the community. METHODS: Archived human sera (7461) collected between 2011 and 2016 from healthy adult blood donors from 50 different nationalities in the western part of Saudi Arabia were obtained for MERS-CoV seroprevalence investigation. Samples were tested for MERS-CoV S1-specific antibodies (Abs) by ELISA and confirmed by testing for neutralizing Abs (nAbs) using both pseudotyped and live virus neutralization assays. RESULTS: Out of 7461 samples, 174 sera from individuals with 18 different nationalities were ELISA positive (2.3%, 95% CI 2.0–2.7). Presence of nAbs was confirmed in 17 samples (0.23%, 95% CI 0.1–0.4) of which one sample exhibited positivity in both neutralization assays. Confirmed seropositivity was identified in young (15–44 years) men and women from Saudi Arabia, Egypt, Yemen, Pakistan, Palestine, Sudan, and India without significant preference. CONCLUSIONS: An increasing trend of MERS-CoV seroprevalence was observed in the general population in western Saudi Arabia, suggesting that asymptomatic or mild infections might exist and act as an unrecognized source of infection. Seropositivity of individuals from different nationalities underscores the potential MERS exportation outside of the Arabian Peninsula. Thus, enhanced and continuous surveillance is highly warranted. Published by Elsevier Ltd on behalf of King Saud Bin Abdulaziz University for Health Sciences. 2020-05 2020-01-28 /pmc/articles/PMC7104088/ /pubmed/32005618 http://dx.doi.org/10.1016/j.jiph.2020.01.001 Text en © 2020 Published by Elsevier Ltd on behalf of King Saud Bin Abdulaziz University for Health Sciences. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Degnah, Afnan A. Al-amri, Sawsan S. Hassan, Ahmed M. Almasoud, Abdulrahman S. Mousa, Manar Almahboub, Sarah A. Alhabbab, Rowa Y. Mirza, Ahmed A. Hindawi, Salwa I. Alharbi, Naif Khalaf Azhar, Esam I. Hashem, Anwar M. Seroprevalence of MERS-CoV in healthy adults in western Saudi Arabia, 2011–2016 |
title | Seroprevalence of MERS-CoV in healthy adults in western Saudi Arabia, 2011–2016 |
title_full | Seroprevalence of MERS-CoV in healthy adults in western Saudi Arabia, 2011–2016 |
title_fullStr | Seroprevalence of MERS-CoV in healthy adults in western Saudi Arabia, 2011–2016 |
title_full_unstemmed | Seroprevalence of MERS-CoV in healthy adults in western Saudi Arabia, 2011–2016 |
title_short | Seroprevalence of MERS-CoV in healthy adults in western Saudi Arabia, 2011–2016 |
title_sort | seroprevalence of mers-cov in healthy adults in western saudi arabia, 2011–2016 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7104088/ https://www.ncbi.nlm.nih.gov/pubmed/32005618 http://dx.doi.org/10.1016/j.jiph.2020.01.001 |
work_keys_str_mv | AT degnahafnana seroprevalenceofmerscovinhealthyadultsinwesternsaudiarabia20112016 AT alamrisawsans seroprevalenceofmerscovinhealthyadultsinwesternsaudiarabia20112016 AT hassanahmedm seroprevalenceofmerscovinhealthyadultsinwesternsaudiarabia20112016 AT almasoudabdulrahmans seroprevalenceofmerscovinhealthyadultsinwesternsaudiarabia20112016 AT mousamanar seroprevalenceofmerscovinhealthyadultsinwesternsaudiarabia20112016 AT almahboubsaraha seroprevalenceofmerscovinhealthyadultsinwesternsaudiarabia20112016 AT alhabbabroway seroprevalenceofmerscovinhealthyadultsinwesternsaudiarabia20112016 AT mirzaahmeda seroprevalenceofmerscovinhealthyadultsinwesternsaudiarabia20112016 AT hindawisalwai seroprevalenceofmerscovinhealthyadultsinwesternsaudiarabia20112016 AT alharbinaifkhalaf seroprevalenceofmerscovinhealthyadultsinwesternsaudiarabia20112016 AT azharesami seroprevalenceofmerscovinhealthyadultsinwesternsaudiarabia20112016 AT hashemanwarm seroprevalenceofmerscovinhealthyadultsinwesternsaudiarabia20112016 |