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Use of the Human Coronavirus 2012 (MERS) GeneSig kit for MERS-CoV detection
INTRODUCTION: Mortality due to MERS-CoV infection is common especially among immunocompromised patients. The pathogenesis and the transmission mode of this virus are still not well understood. The name of the virus is derived from the area of its appearance and the genomic sequence that was used in...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7104146/ https://www.ncbi.nlm.nih.gov/pubmed/32289095 http://dx.doi.org/10.1016/j.genrep.2016.04.004 |
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author | Hoteit, Rouba Shammaa, Dina Mahfouz, Rami |
author_facet | Hoteit, Rouba Shammaa, Dina Mahfouz, Rami |
author_sort | Hoteit, Rouba |
collection | PubMed |
description | INTRODUCTION: Mortality due to MERS-CoV infection is common especially among immunocompromised patients. The pathogenesis and the transmission mode of this virus are still not well understood. The name of the virus is derived from the area of its appearance and the genomic sequence that was used in the development of qRT-PCR assays for MERS-CoV detection was retrieved from the first detected case isolate. The employed assays target various regions including the area upstream of the envelope gene (upE) that is used for screening and the open reading frames (ORF) 1a and 1b used for confirmation. AIM: This study assesses the use of a MERS-CoV specific assay for screening of respiratory samples in anticipation of the possible spread of the virus in the region. METHODS: 46 respiratory specimens were tested using the qualitative one-step qRT-PCR GeneSig Human Coronavirus 2012 (MERS) kit (PrimerDesign™). RESULTS: Out of the 46 tested samples, 45 were negative for MERS-CoV and one sample was found MERS-CoV positive. CONCLUSION: The GeneSig Human Coronavirus 2012 (MERS) kit is very useful for the screening of suspected respiratory cases in the Middle East area as well as other regions. |
format | Online Article Text |
id | pubmed-7104146 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71041462020-03-31 Use of the Human Coronavirus 2012 (MERS) GeneSig kit for MERS-CoV detection Hoteit, Rouba Shammaa, Dina Mahfouz, Rami Gene Rep Article INTRODUCTION: Mortality due to MERS-CoV infection is common especially among immunocompromised patients. The pathogenesis and the transmission mode of this virus are still not well understood. The name of the virus is derived from the area of its appearance and the genomic sequence that was used in the development of qRT-PCR assays for MERS-CoV detection was retrieved from the first detected case isolate. The employed assays target various regions including the area upstream of the envelope gene (upE) that is used for screening and the open reading frames (ORF) 1a and 1b used for confirmation. AIM: This study assesses the use of a MERS-CoV specific assay for screening of respiratory samples in anticipation of the possible spread of the virus in the region. METHODS: 46 respiratory specimens were tested using the qualitative one-step qRT-PCR GeneSig Human Coronavirus 2012 (MERS) kit (PrimerDesign™). RESULTS: Out of the 46 tested samples, 45 were negative for MERS-CoV and one sample was found MERS-CoV positive. CONCLUSION: The GeneSig Human Coronavirus 2012 (MERS) kit is very useful for the screening of suspected respiratory cases in the Middle East area as well as other regions. Elsevier Inc. 2016-09 2016-04-16 /pmc/articles/PMC7104146/ /pubmed/32289095 http://dx.doi.org/10.1016/j.genrep.2016.04.004 Text en © 2016 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Hoteit, Rouba Shammaa, Dina Mahfouz, Rami Use of the Human Coronavirus 2012 (MERS) GeneSig kit for MERS-CoV detection |
title | Use of the Human Coronavirus 2012 (MERS) GeneSig kit for MERS-CoV detection |
title_full | Use of the Human Coronavirus 2012 (MERS) GeneSig kit for MERS-CoV detection |
title_fullStr | Use of the Human Coronavirus 2012 (MERS) GeneSig kit for MERS-CoV detection |
title_full_unstemmed | Use of the Human Coronavirus 2012 (MERS) GeneSig kit for MERS-CoV detection |
title_short | Use of the Human Coronavirus 2012 (MERS) GeneSig kit for MERS-CoV detection |
title_sort | use of the human coronavirus 2012 (mers) genesig kit for mers-cov detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7104146/ https://www.ncbi.nlm.nih.gov/pubmed/32289095 http://dx.doi.org/10.1016/j.genrep.2016.04.004 |
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