Diversity of Bradyrhizobium in Non-Leguminous Sorghum Plants: B. ottawaense Isolates Unique in Genes for N(2)O Reductase and Lack of the Type VI Secretion System

Diverse members of Bradyrhizobium diazoefficiens, B. japonicum, and B. ottawaense were isolated from the roots of field-grown sorghum plants in Fukushima, and classified into “Rhizobia” with nodulated soybeans, “Free-living diazotrophs”, and “Non-diazotrophs” by nitrogen fixation and nodulation assa...

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Autores principales: Wasai-Hara, Sawa, Hara, Shintaro, Morikawa, Takashi, Sugawara, Masayuki, Takami, Hideto, Yoneda, Junich, Tokunaga, Tsuyoshi, Minamisawa, Kiwamu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7104290/
https://www.ncbi.nlm.nih.gov/pubmed/31932539
http://dx.doi.org/10.1264/jsme2.ME19102
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author Wasai-Hara, Sawa
Hara, Shintaro
Morikawa, Takashi
Sugawara, Masayuki
Takami, Hideto
Yoneda, Junich
Tokunaga, Tsuyoshi
Minamisawa, Kiwamu
author_facet Wasai-Hara, Sawa
Hara, Shintaro
Morikawa, Takashi
Sugawara, Masayuki
Takami, Hideto
Yoneda, Junich
Tokunaga, Tsuyoshi
Minamisawa, Kiwamu
author_sort Wasai-Hara, Sawa
collection PubMed
description Diverse members of Bradyrhizobium diazoefficiens, B. japonicum, and B. ottawaense were isolated from the roots of field-grown sorghum plants in Fukushima, and classified into “Rhizobia” with nodulated soybeans, “Free-living diazotrophs”, and “Non-diazotrophs” by nitrogen fixation and nodulation assays. Genome analyses revealed that B. ottawaense members possessed genes for N(2)O reduction, but lacked those for the Type VI secretion system (T6SS). T6SS is a new bacterial weapon against microbial competitors. Since T6SS-possessing B. diazoefficiens and B. japonicum have mainly been isolated from soybean nodules in Japan, T6SS-lacking B. ottawaense members may be a cryptic lineage of soybean bradyrhizobia in Japan.
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spelling pubmed-71042902020-04-06 Diversity of Bradyrhizobium in Non-Leguminous Sorghum Plants: B. ottawaense Isolates Unique in Genes for N(2)O Reductase and Lack of the Type VI Secretion System Wasai-Hara, Sawa Hara, Shintaro Morikawa, Takashi Sugawara, Masayuki Takami, Hideto Yoneda, Junich Tokunaga, Tsuyoshi Minamisawa, Kiwamu Microbes Environ Short Communication Diverse members of Bradyrhizobium diazoefficiens, B. japonicum, and B. ottawaense were isolated from the roots of field-grown sorghum plants in Fukushima, and classified into “Rhizobia” with nodulated soybeans, “Free-living diazotrophs”, and “Non-diazotrophs” by nitrogen fixation and nodulation assays. Genome analyses revealed that B. ottawaense members possessed genes for N(2)O reduction, but lacked those for the Type VI secretion system (T6SS). T6SS is a new bacterial weapon against microbial competitors. Since T6SS-possessing B. diazoefficiens and B. japonicum have mainly been isolated from soybean nodules in Japan, T6SS-lacking B. ottawaense members may be a cryptic lineage of soybean bradyrhizobia in Japan. Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2020 2020-01-11 /pmc/articles/PMC7104290/ /pubmed/31932539 http://dx.doi.org/10.1264/jsme2.ME19102 Text en 2020 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Wasai-Hara, Sawa
Hara, Shintaro
Morikawa, Takashi
Sugawara, Masayuki
Takami, Hideto
Yoneda, Junich
Tokunaga, Tsuyoshi
Minamisawa, Kiwamu
Diversity of Bradyrhizobium in Non-Leguminous Sorghum Plants: B. ottawaense Isolates Unique in Genes for N(2)O Reductase and Lack of the Type VI Secretion System
title Diversity of Bradyrhizobium in Non-Leguminous Sorghum Plants: B. ottawaense Isolates Unique in Genes for N(2)O Reductase and Lack of the Type VI Secretion System
title_full Diversity of Bradyrhizobium in Non-Leguminous Sorghum Plants: B. ottawaense Isolates Unique in Genes for N(2)O Reductase and Lack of the Type VI Secretion System
title_fullStr Diversity of Bradyrhizobium in Non-Leguminous Sorghum Plants: B. ottawaense Isolates Unique in Genes for N(2)O Reductase and Lack of the Type VI Secretion System
title_full_unstemmed Diversity of Bradyrhizobium in Non-Leguminous Sorghum Plants: B. ottawaense Isolates Unique in Genes for N(2)O Reductase and Lack of the Type VI Secretion System
title_short Diversity of Bradyrhizobium in Non-Leguminous Sorghum Plants: B. ottawaense Isolates Unique in Genes for N(2)O Reductase and Lack of the Type VI Secretion System
title_sort diversity of bradyrhizobium in non-leguminous sorghum plants: b. ottawaense isolates unique in genes for n(2)o reductase and lack of the type vi secretion system
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7104290/
https://www.ncbi.nlm.nih.gov/pubmed/31932539
http://dx.doi.org/10.1264/jsme2.ME19102
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