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Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis
BACKGROUND: Innate lymphoid cells (ILCs) play an essential role in maintaining homeostasis; however, they can also cause chronic inflammation and autoimmune disease. This study aimed to identify the role of ILCs in the pathogenesis of lupus nephritis (LN). METHODS: The percentage of ILCs within the...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7104540/ https://www.ncbi.nlm.nih.gov/pubmed/32223753 http://dx.doi.org/10.1186/s13075-020-2114-5 |
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author | Ryu, Seungwon Lee, Eun Young Kim, Dong Ki Kim, Yon Su Chung, Doo Hyun Kim, Ji Hyung Lee, Hajeong Kim, Hye Young |
author_facet | Ryu, Seungwon Lee, Eun Young Kim, Dong Ki Kim, Yon Su Chung, Doo Hyun Kim, Ji Hyung Lee, Hajeong Kim, Hye Young |
author_sort | Ryu, Seungwon |
collection | PubMed |
description | BACKGROUND: Innate lymphoid cells (ILCs) play an essential role in maintaining homeostasis; however, they can also cause chronic inflammation and autoimmune disease. This study aimed to identify the role of ILCs in the pathogenesis of lupus nephritis (LN). METHODS: The percentage of ILCs within the peripheral blood mononuclear cell (PBMC) population and urine of patients with LN (n = 16), healthy controls (HC; n = 8), and disease controls (ANCA-associated vasculitis (AAV; n = 6), IgA nephropathy (IgAN; n = 9), and other glomerular diseases (n = 5)) was determined by flow cytometry analysis. In addition, ILCs were sorted and cultured with plasma from LN patients or HC to elucidate whether the reduced population of CD117(+) ILCs observed in LN was due to changes in the ILC progenitor population. RESULTS: The percentage of total ILCs and CD117(+) ILCs in LN was significantly lower than that in HC. The percentage of cytokine-secreting ILCs was also lower in LN; however, when the disease stabilized, cytokine production was restored to levels similar to those in HC. The increase in the number of exhausted ILCs (cells unable to secrete cytokines) correlated positively with disease activity. When CD117(+) ILCs were cultured with LN plasma, the number of CD117(+) ILCs fell, but that of other ILC subsets increased. CONCLUSIONS: The percentage of CD117(+) ILCs and the capacity of ILCs to secrete cytokines fell as LN severity increased, suggesting that an inflammatory environment of LN induces persistent differentiation and exhaustion of ILCs. |
format | Online Article Text |
id | pubmed-7104540 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-71045402020-03-31 Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis Ryu, Seungwon Lee, Eun Young Kim, Dong Ki Kim, Yon Su Chung, Doo Hyun Kim, Ji Hyung Lee, Hajeong Kim, Hye Young Arthritis Res Ther Research Article BACKGROUND: Innate lymphoid cells (ILCs) play an essential role in maintaining homeostasis; however, they can also cause chronic inflammation and autoimmune disease. This study aimed to identify the role of ILCs in the pathogenesis of lupus nephritis (LN). METHODS: The percentage of ILCs within the peripheral blood mononuclear cell (PBMC) population and urine of patients with LN (n = 16), healthy controls (HC; n = 8), and disease controls (ANCA-associated vasculitis (AAV; n = 6), IgA nephropathy (IgAN; n = 9), and other glomerular diseases (n = 5)) was determined by flow cytometry analysis. In addition, ILCs were sorted and cultured with plasma from LN patients or HC to elucidate whether the reduced population of CD117(+) ILCs observed in LN was due to changes in the ILC progenitor population. RESULTS: The percentage of total ILCs and CD117(+) ILCs in LN was significantly lower than that in HC. The percentage of cytokine-secreting ILCs was also lower in LN; however, when the disease stabilized, cytokine production was restored to levels similar to those in HC. The increase in the number of exhausted ILCs (cells unable to secrete cytokines) correlated positively with disease activity. When CD117(+) ILCs were cultured with LN plasma, the number of CD117(+) ILCs fell, but that of other ILC subsets increased. CONCLUSIONS: The percentage of CD117(+) ILCs and the capacity of ILCs to secrete cytokines fell as LN severity increased, suggesting that an inflammatory environment of LN induces persistent differentiation and exhaustion of ILCs. BioMed Central 2020-03-29 2020 /pmc/articles/PMC7104540/ /pubmed/32223753 http://dx.doi.org/10.1186/s13075-020-2114-5 Text en © The Author(s). 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Ryu, Seungwon Lee, Eun Young Kim, Dong Ki Kim, Yon Su Chung, Doo Hyun Kim, Ji Hyung Lee, Hajeong Kim, Hye Young Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis |
title | Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis |
title_full | Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis |
title_fullStr | Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis |
title_full_unstemmed | Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis |
title_short | Reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis |
title_sort | reduction of circulating innate lymphoid cell progenitors results in impaired cytokine production by innate lymphoid cells in patients with lupus nephritis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7104540/ https://www.ncbi.nlm.nih.gov/pubmed/32223753 http://dx.doi.org/10.1186/s13075-020-2114-5 |
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