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A novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids

Mesoporous aerogel was produced under regular atmospheric conditions using the sol–gel polymerization of tetraethyl orthosilicate with an ionic liquid as both solvent and active agent. This was then used to build a three-dimensional structure to recognize nucleotide acids. Fourier transformation inf...

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Autores principales: Li, Yen Kuang, Yang, Den-Kai, Chen, Yun-Chu, Su, Hung-Ju, Wu, Jui-Chuang, Chen-Yang, Yui Whei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Acta Materialia Inc. Published by Elsevier Ltd. 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105051/
https://www.ncbi.nlm.nih.gov/pubmed/19818421
http://dx.doi.org/10.1016/j.actbio.2009.10.001
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author Li, Yen Kuang
Yang, Den-Kai
Chen, Yun-Chu
Su, Hung-Ju
Wu, Jui-Chuang
Chen-Yang, Yui Whei
author_facet Li, Yen Kuang
Yang, Den-Kai
Chen, Yun-Chu
Su, Hung-Ju
Wu, Jui-Chuang
Chen-Yang, Yui Whei
author_sort Li, Yen Kuang
collection PubMed
description Mesoporous aerogel was produced under regular atmospheric conditions using the sol–gel polymerization of tetraethyl orthosilicate with an ionic liquid as both solvent and active agent. This was then used to build a three-dimensional structure to recognize nucleotide acids. Fourier transformation infrared spectroscopy, scanning electron microscopy, (29)Si solid-state nuclear magnetic resonance, and Brunauer–Emmett–Teller instruments were used to characterize this 3D aerogel, demonstrating that it had high porosity and large internal networking surface area that could capture nucleotide acids. The functionality of molecular recognition on nucleotide acids was demonstrated by immobilizing an oligonucleotide to probe its DNA target and confirming the tagged fluorescent signals by confocal laser scanning microscopy. The results indicated that the as-prepared 3D bioaerogel was capable of providing a very large surface area to capture and recognize human gene ATP5O.
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spelling pubmed-71050512020-03-31 A novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids Li, Yen Kuang Yang, Den-Kai Chen, Yun-Chu Su, Hung-Ju Wu, Jui-Chuang Chen-Yang, Yui Whei Acta Biomater Article Mesoporous aerogel was produced under regular atmospheric conditions using the sol–gel polymerization of tetraethyl orthosilicate with an ionic liquid as both solvent and active agent. This was then used to build a three-dimensional structure to recognize nucleotide acids. Fourier transformation infrared spectroscopy, scanning electron microscopy, (29)Si solid-state nuclear magnetic resonance, and Brunauer–Emmett–Teller instruments were used to characterize this 3D aerogel, demonstrating that it had high porosity and large internal networking surface area that could capture nucleotide acids. The functionality of molecular recognition on nucleotide acids was demonstrated by immobilizing an oligonucleotide to probe its DNA target and confirming the tagged fluorescent signals by confocal laser scanning microscopy. The results indicated that the as-prepared 3D bioaerogel was capable of providing a very large surface area to capture and recognize human gene ATP5O. Acta Materialia Inc. Published by Elsevier Ltd. 2010-04 2009-10-08 /pmc/articles/PMC7105051/ /pubmed/19818421 http://dx.doi.org/10.1016/j.actbio.2009.10.001 Text en Copyright © 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Li, Yen Kuang
Yang, Den-Kai
Chen, Yun-Chu
Su, Hung-Ju
Wu, Jui-Chuang
Chen-Yang, Yui Whei
A novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids
title A novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids
title_full A novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids
title_fullStr A novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids
title_full_unstemmed A novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids
title_short A novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids
title_sort novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105051/
https://www.ncbi.nlm.nih.gov/pubmed/19818421
http://dx.doi.org/10.1016/j.actbio.2009.10.001
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