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Identification of human peripheral blood monocyte gene markers for early screening of solid tumors

As cancer mortality is high in most regions of the world, early screening of cancer has become increasingly important. Minimally invasive screening programs that use peripheral blood mononuclear cells (PBMCs) are a new and reliable strategy that can achieve early detection of tumors by identifying m...

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Autores principales: Chen, Siyang, Liu, Menghan, Liang, Bowen, Ge, Shanghua, Peng, Jie, Huang, Haiyue, Xu, Yanmei, Tang, Xiaoli, Deng, Libin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105127/
https://www.ncbi.nlm.nih.gov/pubmed/32226026
http://dx.doi.org/10.1371/journal.pone.0230905
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author Chen, Siyang
Liu, Menghan
Liang, Bowen
Ge, Shanghua
Peng, Jie
Huang, Haiyue
Xu, Yanmei
Tang, Xiaoli
Deng, Libin
author_facet Chen, Siyang
Liu, Menghan
Liang, Bowen
Ge, Shanghua
Peng, Jie
Huang, Haiyue
Xu, Yanmei
Tang, Xiaoli
Deng, Libin
author_sort Chen, Siyang
collection PubMed
description As cancer mortality is high in most regions of the world, early screening of cancer has become increasingly important. Minimally invasive screening programs that use peripheral blood mononuclear cells (PBMCs) are a new and reliable strategy that can achieve early detection of tumors by identifying marker genes. From 797 datasets, four (GSE12771, GSE24536, GSE27562, and GSE42834) including 428 samples, 236 solid tumor cases, and 192 healthy controls were chosen according to the inclusion criteria. A total of 285 genes from among 440 reported genes were selected by meta-analysis. Among them, 4 of the top significantly differentially expressed genes (ANXA1, IFI44, IFI44L, and OAS1) were identified as marker genes of PBMCs. Pathway enrichment analysis identified, two significant pathways, the ‘primary immunodeficiency’ pathway and the ‘cytokine-cytokine receptor interaction’ pathway. Protein- protein interaction (PPI) network analysis revealed the top 27 hubs with a degree centrality greater than 23 to be hub genes. We also identified 3 modules in Molecular Complex Detection (MCODE) analysis: Cluster 1 (related to ANXA1), Cluster 2 (related to IFI44 and IFI44L) and Cluster 3 (related to OAS1). Among the 4 marker genes, IFI44, IFI44L, and OAS1 are potential diagnostic biomarkers, even though their results were not as remarkable as those for ANXA1 in our study. ANXA1 is involved in the immunosuppressive mechanism in tumor-bearing hosts and may be used in a new strategy involving the use of the host's own immunity to achieve tumor suppression.
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spelling pubmed-71051272020-04-03 Identification of human peripheral blood monocyte gene markers for early screening of solid tumors Chen, Siyang Liu, Menghan Liang, Bowen Ge, Shanghua Peng, Jie Huang, Haiyue Xu, Yanmei Tang, Xiaoli Deng, Libin PLoS One Research Article As cancer mortality is high in most regions of the world, early screening of cancer has become increasingly important. Minimally invasive screening programs that use peripheral blood mononuclear cells (PBMCs) are a new and reliable strategy that can achieve early detection of tumors by identifying marker genes. From 797 datasets, four (GSE12771, GSE24536, GSE27562, and GSE42834) including 428 samples, 236 solid tumor cases, and 192 healthy controls were chosen according to the inclusion criteria. A total of 285 genes from among 440 reported genes were selected by meta-analysis. Among them, 4 of the top significantly differentially expressed genes (ANXA1, IFI44, IFI44L, and OAS1) were identified as marker genes of PBMCs. Pathway enrichment analysis identified, two significant pathways, the ‘primary immunodeficiency’ pathway and the ‘cytokine-cytokine receptor interaction’ pathway. Protein- protein interaction (PPI) network analysis revealed the top 27 hubs with a degree centrality greater than 23 to be hub genes. We also identified 3 modules in Molecular Complex Detection (MCODE) analysis: Cluster 1 (related to ANXA1), Cluster 2 (related to IFI44 and IFI44L) and Cluster 3 (related to OAS1). Among the 4 marker genes, IFI44, IFI44L, and OAS1 are potential diagnostic biomarkers, even though their results were not as remarkable as those for ANXA1 in our study. ANXA1 is involved in the immunosuppressive mechanism in tumor-bearing hosts and may be used in a new strategy involving the use of the host's own immunity to achieve tumor suppression. Public Library of Science 2020-03-30 /pmc/articles/PMC7105127/ /pubmed/32226026 http://dx.doi.org/10.1371/journal.pone.0230905 Text en © 2020 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Chen, Siyang
Liu, Menghan
Liang, Bowen
Ge, Shanghua
Peng, Jie
Huang, Haiyue
Xu, Yanmei
Tang, Xiaoli
Deng, Libin
Identification of human peripheral blood monocyte gene markers for early screening of solid tumors
title Identification of human peripheral blood monocyte gene markers for early screening of solid tumors
title_full Identification of human peripheral blood monocyte gene markers for early screening of solid tumors
title_fullStr Identification of human peripheral blood monocyte gene markers for early screening of solid tumors
title_full_unstemmed Identification of human peripheral blood monocyte gene markers for early screening of solid tumors
title_short Identification of human peripheral blood monocyte gene markers for early screening of solid tumors
title_sort identification of human peripheral blood monocyte gene markers for early screening of solid tumors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105127/
https://www.ncbi.nlm.nih.gov/pubmed/32226026
http://dx.doi.org/10.1371/journal.pone.0230905
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