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Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human
Middle East Respiratory Syndrome Corona Virus (MERSCoV) have been reported in Arabian peninsula and sporadic cases in Europe and Asia. This study was conducted to evaluate the genetic analysis of this virus in human and camel at the first time in Iraq. Two hundred samples were collected from camels...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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IOP Publishing
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105153/ https://www.ncbi.nlm.nih.gov/pubmed/32288777 http://dx.doi.org/10.1088/1742-6596/1294/6/062097 |
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author | Alsalihi, Saba F Abdelkadhim Jawad, Alaa Al-Rodhan, Mohsen A |
author_facet | Alsalihi, Saba F Abdelkadhim Jawad, Alaa Al-Rodhan, Mohsen A |
author_sort | Alsalihi, Saba F |
collection | PubMed |
description | Middle East Respiratory Syndrome Corona Virus (MERSCoV) have been reported in Arabian peninsula and sporadic cases in Europe and Asia. This study was conducted to evaluate the genetic analysis of this virus in human and camel at the first time in Iraq. Two hundred samples were collected from camels and human who suffering from respiratory symptoms, these samples treated with RNA extraction kit then amplification the genetic material by PCR which give 5% positive results. The amplicon then sequenced, registration in gene bank of NCBI for getting accession numbers. The local strains give close relationship with neighbor countries as Saudi Arabia and Jordan strains when using MEGA analysis software. |
format | Online Article Text |
id | pubmed-7105153 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | IOP Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-71051532020-04-03 Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human Alsalihi, Saba F Abdelkadhim Jawad, Alaa Al-Rodhan, Mohsen A J Phys Conf Ser Paper Middle East Respiratory Syndrome Corona Virus (MERSCoV) have been reported in Arabian peninsula and sporadic cases in Europe and Asia. This study was conducted to evaluate the genetic analysis of this virus in human and camel at the first time in Iraq. Two hundred samples were collected from camels and human who suffering from respiratory symptoms, these samples treated with RNA extraction kit then amplification the genetic material by PCR which give 5% positive results. The amplicon then sequenced, registration in gene bank of NCBI for getting accession numbers. The local strains give close relationship with neighbor countries as Saudi Arabia and Jordan strains when using MEGA analysis software. IOP Publishing 2019-09 /pmc/articles/PMC7105153/ /pubmed/32288777 http://dx.doi.org/10.1088/1742-6596/1294/6/062097 Text en Published under licence by IOP Publishing Ltd This article is made available via the PMC Open Access Subset for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. http://creativecommons.org/licenses/by/3.0/ Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence (http://creativecommons.org/licenses/by/3.0) . Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. |
spellingShingle | Paper Alsalihi, Saba F Abdelkadhim Jawad, Alaa Al-Rodhan, Mohsen A Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human |
title | Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human |
title_full | Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human |
title_fullStr | Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human |
title_full_unstemmed | Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human |
title_short | Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human |
title_sort | molecular study and phylogenetic analysis of middle east respiratory syndrome corona virus (merscov) in camel and human |
topic | Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105153/ https://www.ncbi.nlm.nih.gov/pubmed/32288777 http://dx.doi.org/10.1088/1742-6596/1294/6/062097 |
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