Cargando…

Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human

Middle East Respiratory Syndrome Corona Virus (MERSCoV) have been reported in Arabian peninsula and sporadic cases in Europe and Asia. This study was conducted to evaluate the genetic analysis of this virus in human and camel at the first time in Iraq. Two hundred samples were collected from camels...

Descripción completa

Detalles Bibliográficos
Autores principales: Alsalihi, Saba F, Abdelkadhim Jawad, Alaa, Al-Rodhan, Mohsen A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOP Publishing 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105153/
https://www.ncbi.nlm.nih.gov/pubmed/32288777
http://dx.doi.org/10.1088/1742-6596/1294/6/062097
_version_ 1783512353322565632
author Alsalihi, Saba F
Abdelkadhim Jawad, Alaa
Al-Rodhan, Mohsen A
author_facet Alsalihi, Saba F
Abdelkadhim Jawad, Alaa
Al-Rodhan, Mohsen A
author_sort Alsalihi, Saba F
collection PubMed
description Middle East Respiratory Syndrome Corona Virus (MERSCoV) have been reported in Arabian peninsula and sporadic cases in Europe and Asia. This study was conducted to evaluate the genetic analysis of this virus in human and camel at the first time in Iraq. Two hundred samples were collected from camels and human who suffering from respiratory symptoms, these samples treated with RNA extraction kit then amplification the genetic material by PCR which give 5% positive results. The amplicon then sequenced, registration in gene bank of NCBI for getting accession numbers. The local strains give close relationship with neighbor countries as Saudi Arabia and Jordan strains when using MEGA analysis software.
format Online
Article
Text
id pubmed-7105153
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher IOP Publishing
record_format MEDLINE/PubMed
spelling pubmed-71051532020-04-03 Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human Alsalihi, Saba F Abdelkadhim Jawad, Alaa Al-Rodhan, Mohsen A J Phys Conf Ser Paper Middle East Respiratory Syndrome Corona Virus (MERSCoV) have been reported in Arabian peninsula and sporadic cases in Europe and Asia. This study was conducted to evaluate the genetic analysis of this virus in human and camel at the first time in Iraq. Two hundred samples were collected from camels and human who suffering from respiratory symptoms, these samples treated with RNA extraction kit then amplification the genetic material by PCR which give 5% positive results. The amplicon then sequenced, registration in gene bank of NCBI for getting accession numbers. The local strains give close relationship with neighbor countries as Saudi Arabia and Jordan strains when using MEGA analysis software. IOP Publishing 2019-09 /pmc/articles/PMC7105153/ /pubmed/32288777 http://dx.doi.org/10.1088/1742-6596/1294/6/062097 Text en Published under licence by IOP Publishing Ltd This article is made available via the PMC Open Access Subset for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. http://creativecommons.org/licenses/by/3.0/ Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence (http://creativecommons.org/licenses/by/3.0) . Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
spellingShingle Paper
Alsalihi, Saba F
Abdelkadhim Jawad, Alaa
Al-Rodhan, Mohsen A
Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human
title Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human
title_full Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human
title_fullStr Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human
title_full_unstemmed Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human
title_short Molecular Study and Phylogenetic Analysis of Middle East Respiratory Syndrome Corona Virus (MERSCoV) in Camel and Human
title_sort molecular study and phylogenetic analysis of middle east respiratory syndrome corona virus (merscov) in camel and human
topic Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105153/
https://www.ncbi.nlm.nih.gov/pubmed/32288777
http://dx.doi.org/10.1088/1742-6596/1294/6/062097
work_keys_str_mv AT alsalihisabaf molecularstudyandphylogeneticanalysisofmiddleeastrespiratorysyndromecoronavirusmerscovincamelandhuman
AT abdelkadhimjawadalaa molecularstudyandphylogeneticanalysisofmiddleeastrespiratorysyndromecoronavirusmerscovincamelandhuman
AT alrodhanmohsena molecularstudyandphylogeneticanalysisofmiddleeastrespiratorysyndromecoronavirusmerscovincamelandhuman