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Feasibility of parental collected nasal swabs for virus detection in young children with cystic fibrosis
BACKGROUND: The detrimental role of viruses has been well described in CF, although the pattern of virus infections has not been investigated in a longitudinal study. The primary aim was to determine the feasibility of fortnightly parent collected swabs in young children with CF. METHODS: Children u...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
European Cystic Fibrosis Society. Published by Elsevier B.V.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105194/ https://www.ncbi.nlm.nih.gov/pubmed/24637444 http://dx.doi.org/10.1016/j.jcf.2014.02.009 |
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author | Gangell, C.L. Shackleton, C. Poreddy, S. Kappers, J. Gaydon, J.E. Sloots, T.P. Stick, S.M. Ranganathan, S.C. Sly, P.D. |
author_facet | Gangell, C.L. Shackleton, C. Poreddy, S. Kappers, J. Gaydon, J.E. Sloots, T.P. Stick, S.M. Ranganathan, S.C. Sly, P.D. |
author_sort | Gangell, C.L. |
collection | PubMed |
description | BACKGROUND: The detrimental role of viruses has been well described in CF, although the pattern of virus infections has not been investigated in a longitudinal study. The primary aim was to determine the feasibility of fortnightly parent collected swabs in young children with CF. METHODS: Children under three years with CF were recruited. Nasal swabs were collected by parents every fortnight and during periods of symptoms over 12 months. Nasal swabs were posted and virus detected using real-time PCR. RESULTS: Only 27% of the patients completed the study to 10 months, although 98% of the swabs returned were adequate for analysis. Mould was observed growing on 23% of the returned swabs. There was no evidence to demonstrate relationships with symptoms and viruses, prolonged symptoms, prolonged shedding or patterns of virus infections. CONCLUSIONS: This study highlights the need to further investigate the role of viruses in children with CF using a robust method of frequent collection in children for a longitudinal study, with appropriate storage and shipping techniques to avoid mould growth or other potential contaminants. |
format | Online Article Text |
id | pubmed-7105194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | European Cystic Fibrosis Society. Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71051942020-03-31 Feasibility of parental collected nasal swabs for virus detection in young children with cystic fibrosis Gangell, C.L. Shackleton, C. Poreddy, S. Kappers, J. Gaydon, J.E. Sloots, T.P. Stick, S.M. Ranganathan, S.C. Sly, P.D. J Cyst Fibros Article BACKGROUND: The detrimental role of viruses has been well described in CF, although the pattern of virus infections has not been investigated in a longitudinal study. The primary aim was to determine the feasibility of fortnightly parent collected swabs in young children with CF. METHODS: Children under three years with CF were recruited. Nasal swabs were collected by parents every fortnight and during periods of symptoms over 12 months. Nasal swabs were posted and virus detected using real-time PCR. RESULTS: Only 27% of the patients completed the study to 10 months, although 98% of the swabs returned were adequate for analysis. Mould was observed growing on 23% of the returned swabs. There was no evidence to demonstrate relationships with symptoms and viruses, prolonged symptoms, prolonged shedding or patterns of virus infections. CONCLUSIONS: This study highlights the need to further investigate the role of viruses in children with CF using a robust method of frequent collection in children for a longitudinal study, with appropriate storage and shipping techniques to avoid mould growth or other potential contaminants. European Cystic Fibrosis Society. Published by Elsevier B.V. 2014-12 2014-03-15 /pmc/articles/PMC7105194/ /pubmed/24637444 http://dx.doi.org/10.1016/j.jcf.2014.02.009 Text en Copyright © 2014 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Gangell, C.L. Shackleton, C. Poreddy, S. Kappers, J. Gaydon, J.E. Sloots, T.P. Stick, S.M. Ranganathan, S.C. Sly, P.D. Feasibility of parental collected nasal swabs for virus detection in young children with cystic fibrosis |
title | Feasibility of parental collected nasal swabs for virus detection in young children with cystic fibrosis |
title_full | Feasibility of parental collected nasal swabs for virus detection in young children with cystic fibrosis |
title_fullStr | Feasibility of parental collected nasal swabs for virus detection in young children with cystic fibrosis |
title_full_unstemmed | Feasibility of parental collected nasal swabs for virus detection in young children with cystic fibrosis |
title_short | Feasibility of parental collected nasal swabs for virus detection in young children with cystic fibrosis |
title_sort | feasibility of parental collected nasal swabs for virus detection in young children with cystic fibrosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105194/ https://www.ncbi.nlm.nih.gov/pubmed/24637444 http://dx.doi.org/10.1016/j.jcf.2014.02.009 |
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