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Role of respiratory viruses in pulmonary exacerbations in children with cystic fibrosis

BACKGROUND: The role of respiratory viruses in cystic fibrosis (CF) exacerbations is incompletely understood. METHODS: Cross-sectional study of CF children with a pulmonary exacerbation. Mid-turbinate swabs were tested by a direct immunofluorescent antibody assay and a multiplex PCR panel (ResPlex I...

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Autores principales: Asner, Sandra, Waters, Valerie, Solomon, Melinda, Yau, Yvonne, Richardson, Susan E., Grasemann, Hartmut, Gharabaghi, Farhad, Tran, Dat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Cystic Fibrosis Society. Published by Elsevier B.V. 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105203/
https://www.ncbi.nlm.nih.gov/pubmed/22579414
http://dx.doi.org/10.1016/j.jcf.2012.04.006
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author Asner, Sandra
Waters, Valerie
Solomon, Melinda
Yau, Yvonne
Richardson, Susan E.
Grasemann, Hartmut
Gharabaghi, Farhad
Tran, Dat
author_facet Asner, Sandra
Waters, Valerie
Solomon, Melinda
Yau, Yvonne
Richardson, Susan E.
Grasemann, Hartmut
Gharabaghi, Farhad
Tran, Dat
author_sort Asner, Sandra
collection PubMed
description BACKGROUND: The role of respiratory viruses in cystic fibrosis (CF) exacerbations is incompletely understood. METHODS: Cross-sectional study of CF children with a pulmonary exacerbation. Mid-turbinate swabs were tested by a direct immunofluorescent antibody assay and a multiplex PCR panel (ResPlex II v2.0, Qiagen). Resplex II was also applied to sputum or throat swab samples. Pulmonary function tests and quality of life and severity scores were recorded. Sputum cell counts, bacterial density and cytokines were measured. RESULTS: 26/43 (60.5%) subjects tested positive for at least one respiratory virus by any diagnostic method applied to any sample type. Virus-positive patients were younger (p = 0.047), more likely to be male (p = 0.029), and had higher CF clinical severity (p = 0.041) and lower quality of life (physical) scores (p = 0.023) but similar IL-8, neutrophil percentage and elastase levels. CONCLUSIONS: Compared to non-viral exacerbations, viral-related exacerbations were associated with worse severity and quality of life scores but similar pulmonary inflammation.
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spelling pubmed-71052032020-03-31 Role of respiratory viruses in pulmonary exacerbations in children with cystic fibrosis Asner, Sandra Waters, Valerie Solomon, Melinda Yau, Yvonne Richardson, Susan E. Grasemann, Hartmut Gharabaghi, Farhad Tran, Dat J Cyst Fibros Article BACKGROUND: The role of respiratory viruses in cystic fibrosis (CF) exacerbations is incompletely understood. METHODS: Cross-sectional study of CF children with a pulmonary exacerbation. Mid-turbinate swabs were tested by a direct immunofluorescent antibody assay and a multiplex PCR panel (ResPlex II v2.0, Qiagen). Resplex II was also applied to sputum or throat swab samples. Pulmonary function tests and quality of life and severity scores were recorded. Sputum cell counts, bacterial density and cytokines were measured. RESULTS: 26/43 (60.5%) subjects tested positive for at least one respiratory virus by any diagnostic method applied to any sample type. Virus-positive patients were younger (p = 0.047), more likely to be male (p = 0.029), and had higher CF clinical severity (p = 0.041) and lower quality of life (physical) scores (p = 0.023) but similar IL-8, neutrophil percentage and elastase levels. CONCLUSIONS: Compared to non-viral exacerbations, viral-related exacerbations were associated with worse severity and quality of life scores but similar pulmonary inflammation. European Cystic Fibrosis Society. Published by Elsevier B.V. 2012-09 2012-05-10 /pmc/articles/PMC7105203/ /pubmed/22579414 http://dx.doi.org/10.1016/j.jcf.2012.04.006 Text en Copyright © 2012 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Asner, Sandra
Waters, Valerie
Solomon, Melinda
Yau, Yvonne
Richardson, Susan E.
Grasemann, Hartmut
Gharabaghi, Farhad
Tran, Dat
Role of respiratory viruses in pulmonary exacerbations in children with cystic fibrosis
title Role of respiratory viruses in pulmonary exacerbations in children with cystic fibrosis
title_full Role of respiratory viruses in pulmonary exacerbations in children with cystic fibrosis
title_fullStr Role of respiratory viruses in pulmonary exacerbations in children with cystic fibrosis
title_full_unstemmed Role of respiratory viruses in pulmonary exacerbations in children with cystic fibrosis
title_short Role of respiratory viruses in pulmonary exacerbations in children with cystic fibrosis
title_sort role of respiratory viruses in pulmonary exacerbations in children with cystic fibrosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105203/
https://www.ncbi.nlm.nih.gov/pubmed/22579414
http://dx.doi.org/10.1016/j.jcf.2012.04.006
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