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Purification and kinetic behavior of glucose isomerase from Streptomyces lividans RSU26

Glucose isomerase (GI), an enzyme with deserved high potential in the world market. GI plays a major role in high Fructose Corn Syrup Production (HFCS). HFCS is used as a sweetener in food and pharmaceutical industries. Streptomyces are well-known producers of various industrially valuable enzymes,...

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Autores principales: Rengasamy, Sathya, Subramanian, Mohan Raj, Perumal, Varalakshmi, Ganeshan, Shakambari, Al Khulaifi, Manal M., AL-Shwaiman, Hind A., Elgorban, Abdallah M., Syed, Asad, Thangaprakasam, Ushadevi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105671/
https://www.ncbi.nlm.nih.gov/pubmed/32256173
http://dx.doi.org/10.1016/j.sjbs.2019.12.024
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author Rengasamy, Sathya
Subramanian, Mohan Raj
Perumal, Varalakshmi
Ganeshan, Shakambari
Al Khulaifi, Manal M.
AL-Shwaiman, Hind A.
Elgorban, Abdallah M.
Syed, Asad
Thangaprakasam, Ushadevi
author_facet Rengasamy, Sathya
Subramanian, Mohan Raj
Perumal, Varalakshmi
Ganeshan, Shakambari
Al Khulaifi, Manal M.
AL-Shwaiman, Hind A.
Elgorban, Abdallah M.
Syed, Asad
Thangaprakasam, Ushadevi
author_sort Rengasamy, Sathya
collection PubMed
description Glucose isomerase (GI), an enzyme with deserved high potential in the world market. GI plays a major role in high Fructose Corn Syrup Production (HFCS). HFCS is used as a sweetener in food and pharmaceutical industries. Streptomyces are well-known producers of various industrially valuable enzymes, including Glucose isomerase. Currently, recombinant strains have been available for the production of various enzymes, but it has limitation in the large scale production. Therefore, identifying effective streptomyces strains have emerged. The current study, the novel S. lividans RSU26 was isolated from a marine source and optimized its potential to produce glucose isomerase at different physical and chemical conditions. The optimum pH and temperature for GI and biomass production were 7.5 and 35 °C, respectively at 96 h. Characterization study revealed that the approximate molar mass of GI was 43 kDa for monomeric and 170 kDa for tetrameric forms. Kinetic behavior exhibits Km, and Vmax values for the conversion of fructose to glucose conversion were 48.8 mM and 2.54 U mg(−1) at 50 °C and glucose to fructose were 29.4 mM and 2.38 U mg(−1) at 65 °C protein, respectively. Therefore, the present study suggested that the wild–type S. lividans RSU26 has strong potential to produce glucose isomerase for various industrial applications.
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spelling pubmed-71056712020-03-31 Purification and kinetic behavior of glucose isomerase from Streptomyces lividans RSU26 Rengasamy, Sathya Subramanian, Mohan Raj Perumal, Varalakshmi Ganeshan, Shakambari Al Khulaifi, Manal M. AL-Shwaiman, Hind A. Elgorban, Abdallah M. Syed, Asad Thangaprakasam, Ushadevi Saudi J Biol Sci Article Glucose isomerase (GI), an enzyme with deserved high potential in the world market. GI plays a major role in high Fructose Corn Syrup Production (HFCS). HFCS is used as a sweetener in food and pharmaceutical industries. Streptomyces are well-known producers of various industrially valuable enzymes, including Glucose isomerase. Currently, recombinant strains have been available for the production of various enzymes, but it has limitation in the large scale production. Therefore, identifying effective streptomyces strains have emerged. The current study, the novel S. lividans RSU26 was isolated from a marine source and optimized its potential to produce glucose isomerase at different physical and chemical conditions. The optimum pH and temperature for GI and biomass production were 7.5 and 35 °C, respectively at 96 h. Characterization study revealed that the approximate molar mass of GI was 43 kDa for monomeric and 170 kDa for tetrameric forms. Kinetic behavior exhibits Km, and Vmax values for the conversion of fructose to glucose conversion were 48.8 mM and 2.54 U mg(−1) at 50 °C and glucose to fructose were 29.4 mM and 2.38 U mg(−1) at 65 °C protein, respectively. Therefore, the present study suggested that the wild–type S. lividans RSU26 has strong potential to produce glucose isomerase for various industrial applications. Elsevier 2020-04 2019-12-23 /pmc/articles/PMC7105671/ /pubmed/32256173 http://dx.doi.org/10.1016/j.sjbs.2019.12.024 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Rengasamy, Sathya
Subramanian, Mohan Raj
Perumal, Varalakshmi
Ganeshan, Shakambari
Al Khulaifi, Manal M.
AL-Shwaiman, Hind A.
Elgorban, Abdallah M.
Syed, Asad
Thangaprakasam, Ushadevi
Purification and kinetic behavior of glucose isomerase from Streptomyces lividans RSU26
title Purification and kinetic behavior of glucose isomerase from Streptomyces lividans RSU26
title_full Purification and kinetic behavior of glucose isomerase from Streptomyces lividans RSU26
title_fullStr Purification and kinetic behavior of glucose isomerase from Streptomyces lividans RSU26
title_full_unstemmed Purification and kinetic behavior of glucose isomerase from Streptomyces lividans RSU26
title_short Purification and kinetic behavior of glucose isomerase from Streptomyces lividans RSU26
title_sort purification and kinetic behavior of glucose isomerase from streptomyces lividans rsu26
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105671/
https://www.ncbi.nlm.nih.gov/pubmed/32256173
http://dx.doi.org/10.1016/j.sjbs.2019.12.024
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