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Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum

Karrikins are reported to stimulate seed germination, regulate seedling growth, and increase the seedling vigor in abiotic stress conditions in plants. Nevertheless, how karrikins alleviate abiotic stress remains largely elusive. In this study, we found that karrikin (KAR(1)) could significantly all...

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Autores principales: Shah, Faheem Afzal, Wei, Xiao, Wang, Qiaojian, Liu, Wenbo, Wang, Dongdong, Yao, Yuanyuan, Hu, Hao, Chen, Xue, Huang, Shengwei, Hou, Jinyan, Lu, Ruiju, Liu, Chenghong, Ni, Jun, Wu, Lifang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105677/
https://www.ncbi.nlm.nih.gov/pubmed/32265947
http://dx.doi.org/10.3389/fpls.2020.00216
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author Shah, Faheem Afzal
Wei, Xiao
Wang, Qiaojian
Liu, Wenbo
Wang, Dongdong
Yao, Yuanyuan
Hu, Hao
Chen, Xue
Huang, Shengwei
Hou, Jinyan
Lu, Ruiju
Liu, Chenghong
Ni, Jun
Wu, Lifang
author_facet Shah, Faheem Afzal
Wei, Xiao
Wang, Qiaojian
Liu, Wenbo
Wang, Dongdong
Yao, Yuanyuan
Hu, Hao
Chen, Xue
Huang, Shengwei
Hou, Jinyan
Lu, Ruiju
Liu, Chenghong
Ni, Jun
Wu, Lifang
author_sort Shah, Faheem Afzal
collection PubMed
description Karrikins are reported to stimulate seed germination, regulate seedling growth, and increase the seedling vigor in abiotic stress conditions in plants. Nevertheless, how karrikins alleviate abiotic stress remains largely elusive. In this study, we found that karrikin (KAR(1)) could significantly alleviate both drought and salt stress in the important oil plant Sapium sebiferum. KAR(1) supplementation in growth medium at a nanomolar (nM) concentration was enough to recover seed germination under salt and osmotic stress conditions. One nanomolar of KAR(1) improved seedling biomass, increased the taproot length, and increased the number of lateral roots under abiotic stresses, suggesting that KAR(1) is a potent alleviator of abiotic stresses in plants. Under abiotic stresses, KAR(1)-treated seedlings had a higher activity of the key antioxidative enzymes, such as superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase, in comparison with the control, which leads to a lower level of hydrogen peroxide, malondialdehyde, and electrolyte leakage. Moreover, the metabolome analysis showed that KAR(1) treatment significantly increased the level of organic acids and amino acids, which played important roles in redox homeostasis under stresses, suggesting that karrikins might alleviate abiotic stresses via the regulation of redox homeostasis. Under abiotic stresses, applications of karrikins did not increase the endogenous abscisic acid level but altered the expression of several ABA signaling genes, such as SNF1-RELATED PROTEIN KINASE2.3, SNF1-RELATED PROTEIN KINASE2.6, ABI3, and ABI5, suggesting potential interactions between karrikins and ABA signaling in the stress responses. Conclusively, we not only provided the physiological and molecular evidence to clarify the mechanism of karrikins in the regulation of stress adaptation in S. sebiferum but also showed the potential value of karrikins in agricultural practices, which will lay a foundation for further studies about the role of karrikins in abiotic stress alleviation in plants.
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spelling pubmed-71056772020-04-07 Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum Shah, Faheem Afzal Wei, Xiao Wang, Qiaojian Liu, Wenbo Wang, Dongdong Yao, Yuanyuan Hu, Hao Chen, Xue Huang, Shengwei Hou, Jinyan Lu, Ruiju Liu, Chenghong Ni, Jun Wu, Lifang Front Plant Sci Plant Science Karrikins are reported to stimulate seed germination, regulate seedling growth, and increase the seedling vigor in abiotic stress conditions in plants. Nevertheless, how karrikins alleviate abiotic stress remains largely elusive. In this study, we found that karrikin (KAR(1)) could significantly alleviate both drought and salt stress in the important oil plant Sapium sebiferum. KAR(1) supplementation in growth medium at a nanomolar (nM) concentration was enough to recover seed germination under salt and osmotic stress conditions. One nanomolar of KAR(1) improved seedling biomass, increased the taproot length, and increased the number of lateral roots under abiotic stresses, suggesting that KAR(1) is a potent alleviator of abiotic stresses in plants. Under abiotic stresses, KAR(1)-treated seedlings had a higher activity of the key antioxidative enzymes, such as superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase, in comparison with the control, which leads to a lower level of hydrogen peroxide, malondialdehyde, and electrolyte leakage. Moreover, the metabolome analysis showed that KAR(1) treatment significantly increased the level of organic acids and amino acids, which played important roles in redox homeostasis under stresses, suggesting that karrikins might alleviate abiotic stresses via the regulation of redox homeostasis. Under abiotic stresses, applications of karrikins did not increase the endogenous abscisic acid level but altered the expression of several ABA signaling genes, such as SNF1-RELATED PROTEIN KINASE2.3, SNF1-RELATED PROTEIN KINASE2.6, ABI3, and ABI5, suggesting potential interactions between karrikins and ABA signaling in the stress responses. Conclusively, we not only provided the physiological and molecular evidence to clarify the mechanism of karrikins in the regulation of stress adaptation in S. sebiferum but also showed the potential value of karrikins in agricultural practices, which will lay a foundation for further studies about the role of karrikins in abiotic stress alleviation in plants. Frontiers Media S.A. 2020-03-24 /pmc/articles/PMC7105677/ /pubmed/32265947 http://dx.doi.org/10.3389/fpls.2020.00216 Text en Copyright © 2020 Shah, Wei, Wang, Liu, Wang, Yao, Hu, Chen, Huang, Hou, Lu, Liu, Ni and Wu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Shah, Faheem Afzal
Wei, Xiao
Wang, Qiaojian
Liu, Wenbo
Wang, Dongdong
Yao, Yuanyuan
Hu, Hao
Chen, Xue
Huang, Shengwei
Hou, Jinyan
Lu, Ruiju
Liu, Chenghong
Ni, Jun
Wu, Lifang
Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum
title Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum
title_full Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum
title_fullStr Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum
title_full_unstemmed Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum
title_short Karrikin Improves Osmotic and Salt Stress Tolerance via the Regulation of the Redox Homeostasis in the Oil Plant Sapium sebiferum
title_sort karrikin improves osmotic and salt stress tolerance via the regulation of the redox homeostasis in the oil plant sapium sebiferum
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105677/
https://www.ncbi.nlm.nih.gov/pubmed/32265947
http://dx.doi.org/10.3389/fpls.2020.00216
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