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Nucleic acid amplification-based techniques for pathogen detection and identification
Nucleic acid amplification techniques have revolutionised diagnostic and research industries. Current technologies that allow the detection of amplification in real-time are fast becoming industry standards, particularly in a diagnostic context. In this review, we describe and explore the applicatio...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106022/ https://www.ncbi.nlm.nih.gov/pubmed/16169776 http://dx.doi.org/10.1016/j.meegid.2005.08.004 |
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author | Monis, Paul T Giglio, Steven |
author_facet | Monis, Paul T Giglio, Steven |
author_sort | Monis, Paul T |
collection | PubMed |
description | Nucleic acid amplification techniques have revolutionised diagnostic and research industries. Current technologies that allow the detection of amplification in real-time are fast becoming industry standards, particularly in a diagnostic context. In this review, we describe and explore the application of numerous real-time detection chemistries and amplification techniques for pathogen detection and identification, including the polymerase chain reaction, nucleic acid sequence-based amplification, strand displacement amplification and the ligase chain reaction. The emergence of newer technologies, such as lab-on-a-chip devices and photo-cleavable linkers, is also discussed. |
format | Online Article Text |
id | pubmed-7106022 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71060222020-03-31 Nucleic acid amplification-based techniques for pathogen detection and identification Monis, Paul T Giglio, Steven Infect Genet Evol Article Nucleic acid amplification techniques have revolutionised diagnostic and research industries. Current technologies that allow the detection of amplification in real-time are fast becoming industry standards, particularly in a diagnostic context. In this review, we describe and explore the application of numerous real-time detection chemistries and amplification techniques for pathogen detection and identification, including the polymerase chain reaction, nucleic acid sequence-based amplification, strand displacement amplification and the ligase chain reaction. The emergence of newer technologies, such as lab-on-a-chip devices and photo-cleavable linkers, is also discussed. Published by Elsevier B.V. 2006-01 2005-09-19 /pmc/articles/PMC7106022/ /pubmed/16169776 http://dx.doi.org/10.1016/j.meegid.2005.08.004 Text en Crown copyright © 2005 Published by Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Monis, Paul T Giglio, Steven Nucleic acid amplification-based techniques for pathogen detection and identification |
title | Nucleic acid amplification-based techniques for pathogen detection and identification |
title_full | Nucleic acid amplification-based techniques for pathogen detection and identification |
title_fullStr | Nucleic acid amplification-based techniques for pathogen detection and identification |
title_full_unstemmed | Nucleic acid amplification-based techniques for pathogen detection and identification |
title_short | Nucleic acid amplification-based techniques for pathogen detection and identification |
title_sort | nucleic acid amplification-based techniques for pathogen detection and identification |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106022/ https://www.ncbi.nlm.nih.gov/pubmed/16169776 http://dx.doi.org/10.1016/j.meegid.2005.08.004 |
work_keys_str_mv | AT monispault nucleicacidamplificationbasedtechniquesforpathogendetectionandidentification AT gigliosteven nucleicacidamplificationbasedtechniquesforpathogendetectionandidentification |