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Nucleic acid amplification-based techniques for pathogen detection and identification

Nucleic acid amplification techniques have revolutionised diagnostic and research industries. Current technologies that allow the detection of amplification in real-time are fast becoming industry standards, particularly in a diagnostic context. In this review, we describe and explore the applicatio...

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Detalles Bibliográficos
Autores principales: Monis, Paul T, Giglio, Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106022/
https://www.ncbi.nlm.nih.gov/pubmed/16169776
http://dx.doi.org/10.1016/j.meegid.2005.08.004
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author Monis, Paul T
Giglio, Steven
author_facet Monis, Paul T
Giglio, Steven
author_sort Monis, Paul T
collection PubMed
description Nucleic acid amplification techniques have revolutionised diagnostic and research industries. Current technologies that allow the detection of amplification in real-time are fast becoming industry standards, particularly in a diagnostic context. In this review, we describe and explore the application of numerous real-time detection chemistries and amplification techniques for pathogen detection and identification, including the polymerase chain reaction, nucleic acid sequence-based amplification, strand displacement amplification and the ligase chain reaction. The emergence of newer technologies, such as lab-on-a-chip devices and photo-cleavable linkers, is also discussed.
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spelling pubmed-71060222020-03-31 Nucleic acid amplification-based techniques for pathogen detection and identification Monis, Paul T Giglio, Steven Infect Genet Evol Article Nucleic acid amplification techniques have revolutionised diagnostic and research industries. Current technologies that allow the detection of amplification in real-time are fast becoming industry standards, particularly in a diagnostic context. In this review, we describe and explore the application of numerous real-time detection chemistries and amplification techniques for pathogen detection and identification, including the polymerase chain reaction, nucleic acid sequence-based amplification, strand displacement amplification and the ligase chain reaction. The emergence of newer technologies, such as lab-on-a-chip devices and photo-cleavable linkers, is also discussed. Published by Elsevier B.V. 2006-01 2005-09-19 /pmc/articles/PMC7106022/ /pubmed/16169776 http://dx.doi.org/10.1016/j.meegid.2005.08.004 Text en Crown copyright © 2005 Published by Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Monis, Paul T
Giglio, Steven
Nucleic acid amplification-based techniques for pathogen detection and identification
title Nucleic acid amplification-based techniques for pathogen detection and identification
title_full Nucleic acid amplification-based techniques for pathogen detection and identification
title_fullStr Nucleic acid amplification-based techniques for pathogen detection and identification
title_full_unstemmed Nucleic acid amplification-based techniques for pathogen detection and identification
title_short Nucleic acid amplification-based techniques for pathogen detection and identification
title_sort nucleic acid amplification-based techniques for pathogen detection and identification
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106022/
https://www.ncbi.nlm.nih.gov/pubmed/16169776
http://dx.doi.org/10.1016/j.meegid.2005.08.004
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