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Protection from infection with influenza A H7N9 virus in a mouse model by equine neutralizing F(ab′)(2)

Influenza A H7N9 virus has demonstrated considerable pandemic potential in China ever since early spring 2013. Until now, there have been no specific medicines to treat influenza A H7N9 virus infected patients. Development of a safe and effective H7N9 therapeutic preparation is urgently needed. To t...

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Autores principales: Zhao, Zhongpeng, Fan, Chuanbo, Duan, Yueqiang, Zhang, Liangyan, Li, Min, Yang, Xiaolan, Li, Ruisheng, Yang, Penghui, Wang, Xiliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106124/
https://www.ncbi.nlm.nih.gov/pubmed/25192652
http://dx.doi.org/10.1016/j.intimp.2014.08.019
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author Zhao, Zhongpeng
Fan, Chuanbo
Duan, Yueqiang
Zhang, Liangyan
Li, Min
Yang, Xiaolan
Li, Ruisheng
Yang, Penghui
Wang, Xiliang
author_facet Zhao, Zhongpeng
Fan, Chuanbo
Duan, Yueqiang
Zhang, Liangyan
Li, Min
Yang, Xiaolan
Li, Ruisheng
Yang, Penghui
Wang, Xiliang
author_sort Zhao, Zhongpeng
collection PubMed
description Influenza A H7N9 virus has demonstrated considerable pandemic potential in China ever since early spring 2013. Until now, there have been no specific medicines to treat influenza A H7N9 virus infected patients. Development of a safe and effective H7N9 therapeutic preparation is urgently needed. To this end, we prepared and evaluated the pepsin-digested F(ab′)(2) fragments of serum IgGs from the horses inoculated with a inactivated influenza A H7N9 whole virus antigens. The protective effects of the F(ab′)(2) fragments against H7N9 virus infection were determined in cultured MDCK cells by cytopathic effect (CPE) and evaluated in a BALB/c mouse model by observing death, weight loss and viral load. The in vitro results showed that the F(ab′)(2) fragments had an HI titer of 1:2048 and a neutralization titer of 1: 31,623. The in vivo assays suggested that 600U of the preparations could efficiently protect BALB/c mice from a lethal dose of A/Anhui/01/2013 (H7N9) infection even when administered two days post infection. Thus, this highly purified preparation should be a potential candidate for treating severe patients suffering from influenza A H7N9.
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spelling pubmed-71061242020-03-31 Protection from infection with influenza A H7N9 virus in a mouse model by equine neutralizing F(ab′)(2) Zhao, Zhongpeng Fan, Chuanbo Duan, Yueqiang Zhang, Liangyan Li, Min Yang, Xiaolan Li, Ruisheng Yang, Penghui Wang, Xiliang Int Immunopharmacol Article Influenza A H7N9 virus has demonstrated considerable pandemic potential in China ever since early spring 2013. Until now, there have been no specific medicines to treat influenza A H7N9 virus infected patients. Development of a safe and effective H7N9 therapeutic preparation is urgently needed. To this end, we prepared and evaluated the pepsin-digested F(ab′)(2) fragments of serum IgGs from the horses inoculated with a inactivated influenza A H7N9 whole virus antigens. The protective effects of the F(ab′)(2) fragments against H7N9 virus infection were determined in cultured MDCK cells by cytopathic effect (CPE) and evaluated in a BALB/c mouse model by observing death, weight loss and viral load. The in vitro results showed that the F(ab′)(2) fragments had an HI titer of 1:2048 and a neutralization titer of 1: 31,623. The in vivo assays suggested that 600U of the preparations could efficiently protect BALB/c mice from a lethal dose of A/Anhui/01/2013 (H7N9) infection even when administered two days post infection. Thus, this highly purified preparation should be a potential candidate for treating severe patients suffering from influenza A H7N9. Elsevier B.V. 2014-11 2014-09-02 /pmc/articles/PMC7106124/ /pubmed/25192652 http://dx.doi.org/10.1016/j.intimp.2014.08.019 Text en Copyright © 2014 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Zhao, Zhongpeng
Fan, Chuanbo
Duan, Yueqiang
Zhang, Liangyan
Li, Min
Yang, Xiaolan
Li, Ruisheng
Yang, Penghui
Wang, Xiliang
Protection from infection with influenza A H7N9 virus in a mouse model by equine neutralizing F(ab′)(2)
title Protection from infection with influenza A H7N9 virus in a mouse model by equine neutralizing F(ab′)(2)
title_full Protection from infection with influenza A H7N9 virus in a mouse model by equine neutralizing F(ab′)(2)
title_fullStr Protection from infection with influenza A H7N9 virus in a mouse model by equine neutralizing F(ab′)(2)
title_full_unstemmed Protection from infection with influenza A H7N9 virus in a mouse model by equine neutralizing F(ab′)(2)
title_short Protection from infection with influenza A H7N9 virus in a mouse model by equine neutralizing F(ab′)(2)
title_sort protection from infection with influenza a h7n9 virus in a mouse model by equine neutralizing f(ab′)(2)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106124/
https://www.ncbi.nlm.nih.gov/pubmed/25192652
http://dx.doi.org/10.1016/j.intimp.2014.08.019
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