Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children

BACKGROUND: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA. OBJECTIVE: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection. STUDY DESIGN: HBoV1 antigen...

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Detalles Bibliográficos
Autores principales: Kols, Nicola Isabelle, Aatola, Heli, Peltola, Ville, Xu, Man, Nora-Krukle, Zaiga, Hedman, Klaus, Zvirbliene, Aurelija, Toivola, Hanna, Vuorinen, Tytti, Koskinen, Juha M., Bruning, Andrea H.L., Christensen, Andreas, Söderlund-Venermo, Maria, Koskinen, Janne O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106360/
https://www.ncbi.nlm.nih.gov/pubmed/31521013
http://dx.doi.org/10.1016/j.jcv.2019.09.003
Descripción
Sumario:BACKGROUND: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA. OBJECTIVE: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection. STUDY DESIGN: HBoV1 antigen detection has been compared with quantitative HBoV1 DNA PCR and HBoV1 mRNA RT-PCR. RESULTS AND CONCLUSION: We conclude that HBoV1 antigen detection has higher clinical specificity and positive predictive value than HBoV1 DNA qualitative PCRs, yet a lower sensitivity than HBoV1 mRNA detection. Additionally, HBoV1 antigen detection is beneficial in its rapidity and availability as a point-of-care test.

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