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Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children

BACKGROUND: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA. OBJECTIVE: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection. STUDY DESIGN: HBoV1 antigen...

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Autores principales: Kols, Nicola Isabelle, Aatola, Heli, Peltola, Ville, Xu, Man, Nora-Krukle, Zaiga, Hedman, Klaus, Zvirbliene, Aurelija, Toivola, Hanna, Vuorinen, Tytti, Koskinen, Juha M., Bruning, Andrea H.L., Christensen, Andreas, Söderlund-Venermo, Maria, Koskinen, Janne O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106360/
https://www.ncbi.nlm.nih.gov/pubmed/31521013
http://dx.doi.org/10.1016/j.jcv.2019.09.003
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author Kols, Nicola Isabelle
Aatola, Heli
Peltola, Ville
Xu, Man
Nora-Krukle, Zaiga
Hedman, Klaus
Zvirbliene, Aurelija
Toivola, Hanna
Vuorinen, Tytti
Koskinen, Juha M.
Bruning, Andrea H.L.
Christensen, Andreas
Söderlund-Venermo, Maria
Koskinen, Janne O.
author_facet Kols, Nicola Isabelle
Aatola, Heli
Peltola, Ville
Xu, Man
Nora-Krukle, Zaiga
Hedman, Klaus
Zvirbliene, Aurelija
Toivola, Hanna
Vuorinen, Tytti
Koskinen, Juha M.
Bruning, Andrea H.L.
Christensen, Andreas
Söderlund-Venermo, Maria
Koskinen, Janne O.
author_sort Kols, Nicola Isabelle
collection PubMed
description BACKGROUND: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA. OBJECTIVE: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection. STUDY DESIGN: HBoV1 antigen detection has been compared with quantitative HBoV1 DNA PCR and HBoV1 mRNA RT-PCR. RESULTS AND CONCLUSION: We conclude that HBoV1 antigen detection has higher clinical specificity and positive predictive value than HBoV1 DNA qualitative PCRs, yet a lower sensitivity than HBoV1 mRNA detection. Additionally, HBoV1 antigen detection is beneficial in its rapidity and availability as a point-of-care test.
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spelling pubmed-71063602020-03-31 Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children Kols, Nicola Isabelle Aatola, Heli Peltola, Ville Xu, Man Nora-Krukle, Zaiga Hedman, Klaus Zvirbliene, Aurelija Toivola, Hanna Vuorinen, Tytti Koskinen, Juha M. Bruning, Andrea H.L. Christensen, Andreas Söderlund-Venermo, Maria Koskinen, Janne O. J Clin Virol Article BACKGROUND: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA. OBJECTIVE: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection. STUDY DESIGN: HBoV1 antigen detection has been compared with quantitative HBoV1 DNA PCR and HBoV1 mRNA RT-PCR. RESULTS AND CONCLUSION: We conclude that HBoV1 antigen detection has higher clinical specificity and positive predictive value than HBoV1 DNA qualitative PCRs, yet a lower sensitivity than HBoV1 mRNA detection. Additionally, HBoV1 antigen detection is beneficial in its rapidity and availability as a point-of-care test. Elsevier B.V. 2019-11 2019-09-04 /pmc/articles/PMC7106360/ /pubmed/31521013 http://dx.doi.org/10.1016/j.jcv.2019.09.003 Text en © 2019 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Kols, Nicola Isabelle
Aatola, Heli
Peltola, Ville
Xu, Man
Nora-Krukle, Zaiga
Hedman, Klaus
Zvirbliene, Aurelija
Toivola, Hanna
Vuorinen, Tytti
Koskinen, Juha M.
Bruning, Andrea H.L.
Christensen, Andreas
Söderlund-Venermo, Maria
Koskinen, Janne O.
Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children
title Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children
title_full Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children
title_fullStr Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children
title_full_unstemmed Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children
title_short Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children
title_sort comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106360/
https://www.ncbi.nlm.nih.gov/pubmed/31521013
http://dx.doi.org/10.1016/j.jcv.2019.09.003
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