Cargando…
Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children
BACKGROUND: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA. OBJECTIVE: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection. STUDY DESIGN: HBoV1 antigen...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106360/ https://www.ncbi.nlm.nih.gov/pubmed/31521013 http://dx.doi.org/10.1016/j.jcv.2019.09.003 |
_version_ | 1783512595130482688 |
---|---|
author | Kols, Nicola Isabelle Aatola, Heli Peltola, Ville Xu, Man Nora-Krukle, Zaiga Hedman, Klaus Zvirbliene, Aurelija Toivola, Hanna Vuorinen, Tytti Koskinen, Juha M. Bruning, Andrea H.L. Christensen, Andreas Söderlund-Venermo, Maria Koskinen, Janne O. |
author_facet | Kols, Nicola Isabelle Aatola, Heli Peltola, Ville Xu, Man Nora-Krukle, Zaiga Hedman, Klaus Zvirbliene, Aurelija Toivola, Hanna Vuorinen, Tytti Koskinen, Juha M. Bruning, Andrea H.L. Christensen, Andreas Söderlund-Venermo, Maria Koskinen, Janne O. |
author_sort | Kols, Nicola Isabelle |
collection | PubMed |
description | BACKGROUND: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA. OBJECTIVE: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection. STUDY DESIGN: HBoV1 antigen detection has been compared with quantitative HBoV1 DNA PCR and HBoV1 mRNA RT-PCR. RESULTS AND CONCLUSION: We conclude that HBoV1 antigen detection has higher clinical specificity and positive predictive value than HBoV1 DNA qualitative PCRs, yet a lower sensitivity than HBoV1 mRNA detection. Additionally, HBoV1 antigen detection is beneficial in its rapidity and availability as a point-of-care test. |
format | Online Article Text |
id | pubmed-7106360 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71063602020-03-31 Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children Kols, Nicola Isabelle Aatola, Heli Peltola, Ville Xu, Man Nora-Krukle, Zaiga Hedman, Klaus Zvirbliene, Aurelija Toivola, Hanna Vuorinen, Tytti Koskinen, Juha M. Bruning, Andrea H.L. Christensen, Andreas Söderlund-Venermo, Maria Koskinen, Janne O. J Clin Virol Article BACKGROUND: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA. OBJECTIVE: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection. STUDY DESIGN: HBoV1 antigen detection has been compared with quantitative HBoV1 DNA PCR and HBoV1 mRNA RT-PCR. RESULTS AND CONCLUSION: We conclude that HBoV1 antigen detection has higher clinical specificity and positive predictive value than HBoV1 DNA qualitative PCRs, yet a lower sensitivity than HBoV1 mRNA detection. Additionally, HBoV1 antigen detection is beneficial in its rapidity and availability as a point-of-care test. Elsevier B.V. 2019-11 2019-09-04 /pmc/articles/PMC7106360/ /pubmed/31521013 http://dx.doi.org/10.1016/j.jcv.2019.09.003 Text en © 2019 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Kols, Nicola Isabelle Aatola, Heli Peltola, Ville Xu, Man Nora-Krukle, Zaiga Hedman, Klaus Zvirbliene, Aurelija Toivola, Hanna Vuorinen, Tytti Koskinen, Juha M. Bruning, Andrea H.L. Christensen, Andreas Söderlund-Venermo, Maria Koskinen, Janne O. Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children |
title | Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children |
title_full | Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children |
title_fullStr | Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children |
title_full_unstemmed | Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children |
title_short | Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children |
title_sort | comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106360/ https://www.ncbi.nlm.nih.gov/pubmed/31521013 http://dx.doi.org/10.1016/j.jcv.2019.09.003 |
work_keys_str_mv | AT kolsnicolaisabelle comparisonofphenotypicandgenotypicdiagnosisofacutehumanbocavirus1infectioninchildren AT aatolaheli comparisonofphenotypicandgenotypicdiagnosisofacutehumanbocavirus1infectioninchildren AT peltolaville comparisonofphenotypicandgenotypicdiagnosisofacutehumanbocavirus1infectioninchildren AT xuman comparisonofphenotypicandgenotypicdiagnosisofacutehumanbocavirus1infectioninchildren AT norakruklezaiga comparisonofphenotypicandgenotypicdiagnosisofacutehumanbocavirus1infectioninchildren AT hedmanklaus comparisonofphenotypicandgenotypicdiagnosisofacutehumanbocavirus1infectioninchildren AT zvirblieneaurelija comparisonofphenotypicandgenotypicdiagnosisofacutehumanbocavirus1infectioninchildren AT toivolahanna comparisonofphenotypicandgenotypicdiagnosisofacutehumanbocavirus1infectioninchildren AT vuorinentytti comparisonofphenotypicandgenotypicdiagnosisofacutehumanbocavirus1infectioninchildren AT koskinenjuham comparisonofphenotypicandgenotypicdiagnosisofacutehumanbocavirus1infectioninchildren AT bruningandreahl comparisonofphenotypicandgenotypicdiagnosisofacutehumanbocavirus1infectioninchildren AT christensenandreas comparisonofphenotypicandgenotypicdiagnosisofacutehumanbocavirus1infectioninchildren AT soderlundvenermomaria comparisonofphenotypicandgenotypicdiagnosisofacutehumanbocavirus1infectioninchildren AT koskinenjanneo comparisonofphenotypicandgenotypicdiagnosisofacutehumanbocavirus1infectioninchildren |