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Epidemiology and microbiological investigations of community-acquired pneumonia in children admitted at the emergency department of a university hospital

BACKGROUND: The management of children with community-acquired pneumonia (CAP) is largely influenced by the development of new molecular diagnostic tests that allow the simultaneous detection of a wide range of pathogens. OBJECTIVES: Evaluation of a diagnostic approach including multiplex PCR assays...

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Autores principales: Cantais, Aymeric, Mory, Olivier, Pillet, Sylvie, Verhoeven, Paul O., Bonneau, Julie, Patural, Hugues, Pozzetto, Bruno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106426/
https://www.ncbi.nlm.nih.gov/pubmed/24915939
http://dx.doi.org/10.1016/j.jcv.2014.05.006
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author Cantais, Aymeric
Mory, Olivier
Pillet, Sylvie
Verhoeven, Paul O.
Bonneau, Julie
Patural, Hugues
Pozzetto, Bruno
author_facet Cantais, Aymeric
Mory, Olivier
Pillet, Sylvie
Verhoeven, Paul O.
Bonneau, Julie
Patural, Hugues
Pozzetto, Bruno
author_sort Cantais, Aymeric
collection PubMed
description BACKGROUND: The management of children with community-acquired pneumonia (CAP) is largely influenced by the development of new molecular diagnostic tests that allow the simultaneous detection of a wide range of pathogens. OBJECTIVES: Evaluation of a diagnostic approach including multiplex PCR assays for revisiting the epidemiology and etiology of CAP in children at hospital. STUDY DESIGN: Children of all ages consulting at the Emergency Department of the University hospital of Saint-Etienne, France, during the 2012–2013 winter period were included. In addition to bacterial cultures, the following pathogens were detected using biplex commercially-available rt-PCR tests: adenovirus, respiratory syncytial virus, human metapneumovirus, bocavirus, rhinovirus/enterovirus, coronavirus, influenza viruses A and B, parainfluenza viruses, Mycoplasma pneumoniae and Chlamydophila pneumonia. RESULTS: From 85 patients with CAP, at least one pathogen was identified in 81 cases (95.3%), including 4 bacterial exclusive infections (4.7%), 53 viral exclusive infections (62.4%) and 24 mixed infections (28.2%). Coinfection by at least two viruses was observed in 37 cases (43.5%). Mean age was higher in the case of documented bacterial infection (P < 0.05). In the subgroup of viral exclusive infection, the mean age of severe cases was 2.0 years vs 3.8 years in mild and moderate cases (P < 0.05). CONCLUSIONS: These findings highlight the huge proportion of CAP of viral origin, the high number of co-infection by multiple viruses and the low number of bacterial CAP, notably in children under 5 years, and address the need to re-evaluate the indications of empiric antimicrobial treatment in this age group.
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spelling pubmed-71064262020-03-31 Epidemiology and microbiological investigations of community-acquired pneumonia in children admitted at the emergency department of a university hospital Cantais, Aymeric Mory, Olivier Pillet, Sylvie Verhoeven, Paul O. Bonneau, Julie Patural, Hugues Pozzetto, Bruno J Clin Virol Article BACKGROUND: The management of children with community-acquired pneumonia (CAP) is largely influenced by the development of new molecular diagnostic tests that allow the simultaneous detection of a wide range of pathogens. OBJECTIVES: Evaluation of a diagnostic approach including multiplex PCR assays for revisiting the epidemiology and etiology of CAP in children at hospital. STUDY DESIGN: Children of all ages consulting at the Emergency Department of the University hospital of Saint-Etienne, France, during the 2012–2013 winter period were included. In addition to bacterial cultures, the following pathogens were detected using biplex commercially-available rt-PCR tests: adenovirus, respiratory syncytial virus, human metapneumovirus, bocavirus, rhinovirus/enterovirus, coronavirus, influenza viruses A and B, parainfluenza viruses, Mycoplasma pneumoniae and Chlamydophila pneumonia. RESULTS: From 85 patients with CAP, at least one pathogen was identified in 81 cases (95.3%), including 4 bacterial exclusive infections (4.7%), 53 viral exclusive infections (62.4%) and 24 mixed infections (28.2%). Coinfection by at least two viruses was observed in 37 cases (43.5%). Mean age was higher in the case of documented bacterial infection (P < 0.05). In the subgroup of viral exclusive infection, the mean age of severe cases was 2.0 years vs 3.8 years in mild and moderate cases (P < 0.05). CONCLUSIONS: These findings highlight the huge proportion of CAP of viral origin, the high number of co-infection by multiple viruses and the low number of bacterial CAP, notably in children under 5 years, and address the need to re-evaluate the indications of empiric antimicrobial treatment in this age group. Elsevier B.V. 2014-08 2014-05-22 /pmc/articles/PMC7106426/ /pubmed/24915939 http://dx.doi.org/10.1016/j.jcv.2014.05.006 Text en Copyright © 2014 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Cantais, Aymeric
Mory, Olivier
Pillet, Sylvie
Verhoeven, Paul O.
Bonneau, Julie
Patural, Hugues
Pozzetto, Bruno
Epidemiology and microbiological investigations of community-acquired pneumonia in children admitted at the emergency department of a university hospital
title Epidemiology and microbiological investigations of community-acquired pneumonia in children admitted at the emergency department of a university hospital
title_full Epidemiology and microbiological investigations of community-acquired pneumonia in children admitted at the emergency department of a university hospital
title_fullStr Epidemiology and microbiological investigations of community-acquired pneumonia in children admitted at the emergency department of a university hospital
title_full_unstemmed Epidemiology and microbiological investigations of community-acquired pneumonia in children admitted at the emergency department of a university hospital
title_short Epidemiology and microbiological investigations of community-acquired pneumonia in children admitted at the emergency department of a university hospital
title_sort epidemiology and microbiological investigations of community-acquired pneumonia in children admitted at the emergency department of a university hospital
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106426/
https://www.ncbi.nlm.nih.gov/pubmed/24915939
http://dx.doi.org/10.1016/j.jcv.2014.05.006
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