Evaluation of Seegene Allplex Respiratory Panel 1 kit for the detection of influenza virus and human respiratory syncytial virus

BACKGROUND: Influenza (FLUV) and human respiratory syncytial (HRSV) viruses are etiological agents of respiratory infections that cause a significant morbidity and mortality worldwide. A rapid and accurate diagnosis of these respiratory viruses is essential for an appropriate patient management. Mol...

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Autores principales: Gimferrer, Laura, Andrés, Cristina, Rando, Ariadna, Piñana, Maria, Codina, Maria Gema, Martin, Maria del Carmen, Fuentes, Francisco, Rubio, Susana, Alcubilla, Pilar, Pumarola, Tomàs, Antón, Andrés
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 2018
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106510/
https://www.ncbi.nlm.nih.gov/pubmed/29883908
http://dx.doi.org/10.1016/j.jcv.2018.05.006
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author Gimferrer, Laura
Andrés, Cristina
Rando, Ariadna
Piñana, Maria
Codina, Maria Gema
Martin, Maria del Carmen
Fuentes, Francisco
Rubio, Susana
Alcubilla, Pilar
Pumarola, Tomàs
Antón, Andrés
author_facet Gimferrer, Laura
Andrés, Cristina
Rando, Ariadna
Piñana, Maria
Codina, Maria Gema
Martin, Maria del Carmen
Fuentes, Francisco
Rubio, Susana
Alcubilla, Pilar
Pumarola, Tomàs
Antón, Andrés
author_sort Gimferrer, Laura
collection PubMed
description BACKGROUND: Influenza (FLUV) and human respiratory syncytial (HRSV) viruses are etiological agents of respiratory infections that cause a significant morbidity and mortality worldwide. A rapid and accurate diagnosis of these respiratory viruses is essential for an appropriate patient management. Molecular tests are the best detection option due to their high sensitivity and specificity. Seegene’s Allplex™ Respiratory Panel 1 (Allplex RP1) is a real-time one-step RT-PCR assay for the simultaneous detection of FLUAV, FLUBV, HRSV-A and HRSV-B. In addition, it allows the determination of FLUAV subtype (H1, H3 and H1pdm09). OBJECTIVES: This study aims to evaluate Allplex RP1 as a rapid molecular test for the detection of FLUAV, FLUBV, HRSV-A and HRSV-B viruses. STUDY DESIGN: The Allplex RP1 assay will be compared with other two commercial molecular assays, Prodesse ProFlu+ and ProFAST+ (Hologic, Madison, WI, USA), and GeneXpert Flu/RSV XC (Cepheid, USA). RESULTS: Allplex RP1, ProFlu+ and GeneXpert tests showed 95%, 91% and 96% of accuracy; and 94%, 88% and 95% of sensitivity, respectively. Moreover, Allplex RP1 showed a FLUAV subtype sensitivity of 91% and 88% for FLUAV-H1pdm09 and FLUAV-H3 respectively, and ProFAST+ assay showed sensitivities of 100% for both targets. The three assays showed a 100% of specificity and PPV, while the NPV were 84%, 73% and 86% for Allplex RP1, Prodesse and GeneXpert, respectively. CONCLUSIONS: In this study, Seegene’s Allplex RP1 assay showed to be highly sensitive, specific, and suitable for detection of FLUV and HRSV, including FLUAV subtyping. In addition, it is also a hands-on-time saving assay due to the automated nucleic acid extraction and PCR setup.
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spelling pubmed-71065102020-03-31 Evaluation of Seegene Allplex Respiratory Panel 1 kit for the detection of influenza virus and human respiratory syncytial virus Gimferrer, Laura Andrés, Cristina Rando, Ariadna Piñana, Maria Codina, Maria Gema Martin, Maria del Carmen Fuentes, Francisco Rubio, Susana Alcubilla, Pilar Pumarola, Tomàs Antón, Andrés J Clin Virol Article BACKGROUND: Influenza (FLUV) and human respiratory syncytial (HRSV) viruses are etiological agents of respiratory infections that cause a significant morbidity and mortality worldwide. A rapid and accurate diagnosis of these respiratory viruses is essential for an appropriate patient management. Molecular tests are the best detection option due to their high sensitivity and specificity. Seegene’s Allplex™ Respiratory Panel 1 (Allplex RP1) is a real-time one-step RT-PCR assay for the simultaneous detection of FLUAV, FLUBV, HRSV-A and HRSV-B. In addition, it allows the determination of FLUAV subtype (H1, H3 and H1pdm09). OBJECTIVES: This study aims to evaluate Allplex RP1 as a rapid molecular test for the detection of FLUAV, FLUBV, HRSV-A and HRSV-B viruses. STUDY DESIGN: The Allplex RP1 assay will be compared with other two commercial molecular assays, Prodesse ProFlu+ and ProFAST+ (Hologic, Madison, WI, USA), and GeneXpert Flu/RSV XC (Cepheid, USA). RESULTS: Allplex RP1, ProFlu+ and GeneXpert tests showed 95%, 91% and 96% of accuracy; and 94%, 88% and 95% of sensitivity, respectively. Moreover, Allplex RP1 showed a FLUAV subtype sensitivity of 91% and 88% for FLUAV-H1pdm09 and FLUAV-H3 respectively, and ProFAST+ assay showed sensitivities of 100% for both targets. The three assays showed a 100% of specificity and PPV, while the NPV were 84%, 73% and 86% for Allplex RP1, Prodesse and GeneXpert, respectively. CONCLUSIONS: In this study, Seegene’s Allplex RP1 assay showed to be highly sensitive, specific, and suitable for detection of FLUV and HRSV, including FLUAV subtyping. In addition, it is also a hands-on-time saving assay due to the automated nucleic acid extraction and PCR setup. Published by Elsevier B.V. 2018-08 2018-05-25 /pmc/articles/PMC7106510/ /pubmed/29883908 http://dx.doi.org/10.1016/j.jcv.2018.05.006 Text en © 2018 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Gimferrer, Laura
Andrés, Cristina
Rando, Ariadna
Piñana, Maria
Codina, Maria Gema
Martin, Maria del Carmen
Fuentes, Francisco
Rubio, Susana
Alcubilla, Pilar
Pumarola, Tomàs
Antón, Andrés
Evaluation of Seegene Allplex Respiratory Panel 1 kit for the detection of influenza virus and human respiratory syncytial virus
title Evaluation of Seegene Allplex Respiratory Panel 1 kit for the detection of influenza virus and human respiratory syncytial virus
title_full Evaluation of Seegene Allplex Respiratory Panel 1 kit for the detection of influenza virus and human respiratory syncytial virus
title_fullStr Evaluation of Seegene Allplex Respiratory Panel 1 kit for the detection of influenza virus and human respiratory syncytial virus
title_full_unstemmed Evaluation of Seegene Allplex Respiratory Panel 1 kit for the detection of influenza virus and human respiratory syncytial virus
title_short Evaluation of Seegene Allplex Respiratory Panel 1 kit for the detection of influenza virus and human respiratory syncytial virus
title_sort evaluation of seegene allplex respiratory panel 1 kit for the detection of influenza virus and human respiratory syncytial virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106510/
https://www.ncbi.nlm.nih.gov/pubmed/29883908
http://dx.doi.org/10.1016/j.jcv.2018.05.006
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