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In vitro screening antiviral activity of Thai medicinal plants against porcine reproductive and respiratory syndrome virus
BACKGROUND: Porcine reproductive and respiratory syndrome (PRRS) caused by PRRS virus (PRRSV) results in economic losses in the swine industry globally. Several studies have investigated the use of plant extracts in the prevention and control of PRRS outbreaks. Thai medicinal plants may be useful fo...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106583/ https://www.ncbi.nlm.nih.gov/pubmed/32228582 http://dx.doi.org/10.1186/s12917-020-02320-8 |
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author | Arjin, Chaiwat Pringproa, Kidsadagon Hongsibsong, Surat Ruksiriwanich, Warintorn Seel-audom, Mintra Mekchay, Supamit Sringarm, Korawan |
author_facet | Arjin, Chaiwat Pringproa, Kidsadagon Hongsibsong, Surat Ruksiriwanich, Warintorn Seel-audom, Mintra Mekchay, Supamit Sringarm, Korawan |
author_sort | Arjin, Chaiwat |
collection | PubMed |
description | BACKGROUND: Porcine reproductive and respiratory syndrome (PRRS) caused by PRRS virus (PRRSV) results in economic losses in the swine industry globally. Several studies have investigated the use of plant extracts in the prevention and control of PRRS outbreaks. Thai medicinal plants may be useful for treating PRRSV infection in pigs. Therefore, we investigated the in vitro anti-PRRSV and antioxidant properties of seven Thai medicinal plants: Caesalpinia sappan Linn., Garcinia mangostana Linn., Houttuynia cordata, Perilla frutescens, Clinacanthus nutans, Phyllanthus emblica, and Tiliacora triandra. RESULTS: Using antiviral screening, we observed that T. triandra extract strongly inhibited PRRSV infectivity in MARC-145 cells [virus titer 3.5 median tissue culture infective dose (TCID(50))/ml (log10)] at 24 h post-infection, whereas C. sappan extract strongly inhibited PRRSV replication [virus titer 2.5 TCID(50)/ml (log10)] at 72 h post-infection. C. sappan extract had the highest total phenolic content [220.52 mM gallic acid equivalent/g] and lowest half-maximal inhibitory concentration [1.17 mg/ml in 2,2-diphenyl-1-picrylhydrazyl and 2.58 mg/ml in 2,2-azino-bis (3-ethylbenzothiazo-line-6-sulfonic acid) diammonium salt]. CONCLUSION: T. triandra extract could inhibit PRRSV infectivity, whereas C. sappan extract was the most effective in inhibiting PRRSV replication in MARC-145 cells. This study elucidates the antiviral activities of Thai medicinal plant extracts in vivo. The results promise that Thai medicinal plant extracts, particularly T. triandra and C. sappan extracts, can be developed into pharmaceutical drugs for the prevention of PRRS in pigs. |
format | Online Article Text |
id | pubmed-7106583 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-71065832020-04-01 In vitro screening antiviral activity of Thai medicinal plants against porcine reproductive and respiratory syndrome virus Arjin, Chaiwat Pringproa, Kidsadagon Hongsibsong, Surat Ruksiriwanich, Warintorn Seel-audom, Mintra Mekchay, Supamit Sringarm, Korawan BMC Vet Res Research Article BACKGROUND: Porcine reproductive and respiratory syndrome (PRRS) caused by PRRS virus (PRRSV) results in economic losses in the swine industry globally. Several studies have investigated the use of plant extracts in the prevention and control of PRRS outbreaks. Thai medicinal plants may be useful for treating PRRSV infection in pigs. Therefore, we investigated the in vitro anti-PRRSV and antioxidant properties of seven Thai medicinal plants: Caesalpinia sappan Linn., Garcinia mangostana Linn., Houttuynia cordata, Perilla frutescens, Clinacanthus nutans, Phyllanthus emblica, and Tiliacora triandra. RESULTS: Using antiviral screening, we observed that T. triandra extract strongly inhibited PRRSV infectivity in MARC-145 cells [virus titer 3.5 median tissue culture infective dose (TCID(50))/ml (log10)] at 24 h post-infection, whereas C. sappan extract strongly inhibited PRRSV replication [virus titer 2.5 TCID(50)/ml (log10)] at 72 h post-infection. C. sappan extract had the highest total phenolic content [220.52 mM gallic acid equivalent/g] and lowest half-maximal inhibitory concentration [1.17 mg/ml in 2,2-diphenyl-1-picrylhydrazyl and 2.58 mg/ml in 2,2-azino-bis (3-ethylbenzothiazo-line-6-sulfonic acid) diammonium salt]. CONCLUSION: T. triandra extract could inhibit PRRSV infectivity, whereas C. sappan extract was the most effective in inhibiting PRRSV replication in MARC-145 cells. This study elucidates the antiviral activities of Thai medicinal plant extracts in vivo. The results promise that Thai medicinal plant extracts, particularly T. triandra and C. sappan extracts, can be developed into pharmaceutical drugs for the prevention of PRRS in pigs. BioMed Central 2020-03-30 /pmc/articles/PMC7106583/ /pubmed/32228582 http://dx.doi.org/10.1186/s12917-020-02320-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Arjin, Chaiwat Pringproa, Kidsadagon Hongsibsong, Surat Ruksiriwanich, Warintorn Seel-audom, Mintra Mekchay, Supamit Sringarm, Korawan In vitro screening antiviral activity of Thai medicinal plants against porcine reproductive and respiratory syndrome virus |
title | In vitro screening antiviral activity of Thai medicinal plants against porcine reproductive and respiratory syndrome virus |
title_full | In vitro screening antiviral activity of Thai medicinal plants against porcine reproductive and respiratory syndrome virus |
title_fullStr | In vitro screening antiviral activity of Thai medicinal plants against porcine reproductive and respiratory syndrome virus |
title_full_unstemmed | In vitro screening antiviral activity of Thai medicinal plants against porcine reproductive and respiratory syndrome virus |
title_short | In vitro screening antiviral activity of Thai medicinal plants against porcine reproductive and respiratory syndrome virus |
title_sort | in vitro screening antiviral activity of thai medicinal plants against porcine reproductive and respiratory syndrome virus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106583/ https://www.ncbi.nlm.nih.gov/pubmed/32228582 http://dx.doi.org/10.1186/s12917-020-02320-8 |
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