Downregulation of S100 calcium binding protein A12 inhibits the growth of glioma cells

BACKGROUND: S100 calcium binding protein A12 (S100A12) is a member of the S100 protein family and is widely expressed in neutrophil and low expressed in lymphocytes and monocyte. However, the role of S100A12 in glioma has not yet been identified. METHODS: In the present study, we carried out immunoh...

Descripción completa

Detalles Bibliográficos
Autores principales: Lu, Chunhe, Liu, Jia, Yao, Mingze, Li, Lun, Li, Guangyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106817/
https://www.ncbi.nlm.nih.gov/pubmed/32228516
http://dx.doi.org/10.1186/s12885-020-06768-7
_version_ 1783512693016100864
author Lu, Chunhe
Liu, Jia
Yao, Mingze
Li, Lun
Li, Guangyu
author_facet Lu, Chunhe
Liu, Jia
Yao, Mingze
Li, Lun
Li, Guangyu
author_sort Lu, Chunhe
collection PubMed
description BACKGROUND: S100 calcium binding protein A12 (S100A12) is a member of the S100 protein family and is widely expressed in neutrophil and low expressed in lymphocytes and monocyte. However, the role of S100A12 in glioma has not yet been identified. METHODS: In the present study, we carried out immunohistochemical investigation of S100A12 in 81 glioma tissues to determine the expression of S100A12 in glioma cells, and evaluate the clinical significance of S100A12 in glioma patients. Futher we knockdown the S100A12 by shRNA, and evaluated cell proliferation, cell migration and cell apoptosis by MTT, colony formation assay, transwell assay,flow cytometry assa and western blot. RESULTS: We found that S100A12 was upregulated in tissues of glioma patients and the expression was correlated to WHO stage and tumor size. Further, we found that knockdown S100A12 inhibits the proliferation, migration and invasion of glioma cells through regulating cell apoptosis and EMT. CONCLUSION: S100A12 plays a vital role in glioma progression, and may be an important regulatory molecule for biological behaviors of glioma cell lines.
format Online
Article
Text
id pubmed-7106817
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-71068172020-04-01 Downregulation of S100 calcium binding protein A12 inhibits the growth of glioma cells Lu, Chunhe Liu, Jia Yao, Mingze Li, Lun Li, Guangyu BMC Cancer Research Article BACKGROUND: S100 calcium binding protein A12 (S100A12) is a member of the S100 protein family and is widely expressed in neutrophil and low expressed in lymphocytes and monocyte. However, the role of S100A12 in glioma has not yet been identified. METHODS: In the present study, we carried out immunohistochemical investigation of S100A12 in 81 glioma tissues to determine the expression of S100A12 in glioma cells, and evaluate the clinical significance of S100A12 in glioma patients. Futher we knockdown the S100A12 by shRNA, and evaluated cell proliferation, cell migration and cell apoptosis by MTT, colony formation assay, transwell assay,flow cytometry assa and western blot. RESULTS: We found that S100A12 was upregulated in tissues of glioma patients and the expression was correlated to WHO stage and tumor size. Further, we found that knockdown S100A12 inhibits the proliferation, migration and invasion of glioma cells through regulating cell apoptosis and EMT. CONCLUSION: S100A12 plays a vital role in glioma progression, and may be an important regulatory molecule for biological behaviors of glioma cell lines. BioMed Central 2020-03-30 /pmc/articles/PMC7106817/ /pubmed/32228516 http://dx.doi.org/10.1186/s12885-020-06768-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Lu, Chunhe
Liu, Jia
Yao, Mingze
Li, Lun
Li, Guangyu
Downregulation of S100 calcium binding protein A12 inhibits the growth of glioma cells
title Downregulation of S100 calcium binding protein A12 inhibits the growth of glioma cells
title_full Downregulation of S100 calcium binding protein A12 inhibits the growth of glioma cells
title_fullStr Downregulation of S100 calcium binding protein A12 inhibits the growth of glioma cells
title_full_unstemmed Downregulation of S100 calcium binding protein A12 inhibits the growth of glioma cells
title_short Downregulation of S100 calcium binding protein A12 inhibits the growth of glioma cells
title_sort downregulation of s100 calcium binding protein a12 inhibits the growth of glioma cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106817/
https://www.ncbi.nlm.nih.gov/pubmed/32228516
http://dx.doi.org/10.1186/s12885-020-06768-7
work_keys_str_mv AT luchunhe downregulationofs100calciumbindingproteina12inhibitsthegrowthofgliomacells
AT liujia downregulationofs100calciumbindingproteina12inhibitsthegrowthofgliomacells
AT yaomingze downregulationofs100calciumbindingproteina12inhibitsthegrowthofgliomacells
AT lilun downregulationofs100calciumbindingproteina12inhibitsthegrowthofgliomacells
AT liguangyu downregulationofs100calciumbindingproteina12inhibitsthegrowthofgliomacells

Ejemplares similares