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Selenium-Enriched Yeast Alleviates Oxidative Stress-Induced Intestinal Mucosa Disruption in Weaned Pigs

To explore the effect of selenium-enriched yeast (SeY) on intestinal barrier functions in weaned pigs upon oxidative stress, a 2 × 2 factorial design was utilized and thirty-two pigs were randomly assigned into four groups. Pigs with or without exposure to oxidative stress (diquat challenge) were fe...

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Autores principales: Liu, Lei, Wu, Caimei, Chen, Daiwen, Yu, Bing, Huang, Zhiqing, Luo, Yuheng, Zheng, Ping, Mao, Xiangbing, Yu, Jie, Luo, Junqiu, Yan, Hui, He, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106930/
https://www.ncbi.nlm.nih.gov/pubmed/32256952
http://dx.doi.org/10.1155/2020/5490743
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author Liu, Lei
Wu, Caimei
Chen, Daiwen
Yu, Bing
Huang, Zhiqing
Luo, Yuheng
Zheng, Ping
Mao, Xiangbing
Yu, Jie
Luo, Junqiu
Yan, Hui
He, Jun
author_facet Liu, Lei
Wu, Caimei
Chen, Daiwen
Yu, Bing
Huang, Zhiqing
Luo, Yuheng
Zheng, Ping
Mao, Xiangbing
Yu, Jie
Luo, Junqiu
Yan, Hui
He, Jun
author_sort Liu, Lei
collection PubMed
description To explore the effect of selenium-enriched yeast (SeY) on intestinal barrier functions in weaned pigs upon oxidative stress, a 2 × 2 factorial design was utilized and thirty-two pigs were randomly assigned into four groups. Pigs with or without exposure to oxidative stress (diquat challenge) were fed with a basal diet or a SeY-containing diet. The trial lasted for 21 days, and result showed that SeY supplementation attenuated body-weight reduction and significantly decreased the serum concentrations of diamine oxidase (DAO) and D-lactic acid in pigs upon diquat challenge (P < 0.05). Diquat challenge decreased the villus height and the ratio of villus height to crypt depth (V/C) in the jejunum and ileum (P < 0.05). However, SeY supplementation not only elevated the villus height and the ratio of V/C (P < 0.05) but also improved the distribution and abundance of tight-junction protein ZO-1 in the jejunum epithelium. Interestingly, SeY supplementation acutely decreased the total apoptosis rate of intestinal epithelial cells in pigs upon diquat challenge (P < 0.05). Moreover, SeY elevated the content of antioxidant molecules such as glutathione peroxidase (GSH-Px) and catalase (CAT) but significantly decreased the content of malondialdehyde (MDA) in the intestinal mucosa (P < 0.05). Importantly, SeY elevated the expression levels of critical functional genes such as the nuclear factor erythroid-2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), sodium/glucose cotransporter 1 (SGLT1), and B-cell lymphoma-2 (BCL-2) in the intestinal mucosa upon diquat challenge (P < 0.05). Moreover, the expression of caspase-3 was downregulated by SeY in the duodenum and jejunum mucosa (P < 0.05). These results indicated that SeY attenuated oxidative stress-induced intestinal mucosa disruption, which was associated with elevated mucosal antioxidative capacity and improved intestinal barrier functions.
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spelling pubmed-71069302020-04-03 Selenium-Enriched Yeast Alleviates Oxidative Stress-Induced Intestinal Mucosa Disruption in Weaned Pigs Liu, Lei Wu, Caimei Chen, Daiwen Yu, Bing Huang, Zhiqing Luo, Yuheng Zheng, Ping Mao, Xiangbing Yu, Jie Luo, Junqiu Yan, Hui He, Jun Oxid Med Cell Longev Research Article To explore the effect of selenium-enriched yeast (SeY) on intestinal barrier functions in weaned pigs upon oxidative stress, a 2 × 2 factorial design was utilized and thirty-two pigs were randomly assigned into four groups. Pigs with or without exposure to oxidative stress (diquat challenge) were fed with a basal diet or a SeY-containing diet. The trial lasted for 21 days, and result showed that SeY supplementation attenuated body-weight reduction and significantly decreased the serum concentrations of diamine oxidase (DAO) and D-lactic acid in pigs upon diquat challenge (P < 0.05). Diquat challenge decreased the villus height and the ratio of villus height to crypt depth (V/C) in the jejunum and ileum (P < 0.05). However, SeY supplementation not only elevated the villus height and the ratio of V/C (P < 0.05) but also improved the distribution and abundance of tight-junction protein ZO-1 in the jejunum epithelium. Interestingly, SeY supplementation acutely decreased the total apoptosis rate of intestinal epithelial cells in pigs upon diquat challenge (P < 0.05). Moreover, SeY elevated the content of antioxidant molecules such as glutathione peroxidase (GSH-Px) and catalase (CAT) but significantly decreased the content of malondialdehyde (MDA) in the intestinal mucosa (P < 0.05). Importantly, SeY elevated the expression levels of critical functional genes such as the nuclear factor erythroid-2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), sodium/glucose cotransporter 1 (SGLT1), and B-cell lymphoma-2 (BCL-2) in the intestinal mucosa upon diquat challenge (P < 0.05). Moreover, the expression of caspase-3 was downregulated by SeY in the duodenum and jejunum mucosa (P < 0.05). These results indicated that SeY attenuated oxidative stress-induced intestinal mucosa disruption, which was associated with elevated mucosal antioxidative capacity and improved intestinal barrier functions. Hindawi 2020-03-19 /pmc/articles/PMC7106930/ /pubmed/32256952 http://dx.doi.org/10.1155/2020/5490743 Text en Copyright © 2020 Lei Liu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Lei
Wu, Caimei
Chen, Daiwen
Yu, Bing
Huang, Zhiqing
Luo, Yuheng
Zheng, Ping
Mao, Xiangbing
Yu, Jie
Luo, Junqiu
Yan, Hui
He, Jun
Selenium-Enriched Yeast Alleviates Oxidative Stress-Induced Intestinal Mucosa Disruption in Weaned Pigs
title Selenium-Enriched Yeast Alleviates Oxidative Stress-Induced Intestinal Mucosa Disruption in Weaned Pigs
title_full Selenium-Enriched Yeast Alleviates Oxidative Stress-Induced Intestinal Mucosa Disruption in Weaned Pigs
title_fullStr Selenium-Enriched Yeast Alleviates Oxidative Stress-Induced Intestinal Mucosa Disruption in Weaned Pigs
title_full_unstemmed Selenium-Enriched Yeast Alleviates Oxidative Stress-Induced Intestinal Mucosa Disruption in Weaned Pigs
title_short Selenium-Enriched Yeast Alleviates Oxidative Stress-Induced Intestinal Mucosa Disruption in Weaned Pigs
title_sort selenium-enriched yeast alleviates oxidative stress-induced intestinal mucosa disruption in weaned pigs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106930/
https://www.ncbi.nlm.nih.gov/pubmed/32256952
http://dx.doi.org/10.1155/2020/5490743
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